SH3PXD2A‐AS1/miR‐330‐5p/UBA2 ceRNA network mediates the progression of colorectal cancer through regulating the activity of the Wnt/β‐catenin signaling pathway

Abstract

Long non‐coding RNAs have important roles in the occurrence and progression of various cancers. However, the molecular mechanism of lncRNAs in colorectal cancer (CRC) is not well illustrated. Thus, we used bioinformatics methods to find potential lncRNAs associated with CRC progression, and chose SH3PXD2A‐AS1 as a candidate for further analysis. The roles of SH3PXD2A‐AS1 in CRC cells were determined by CCK‐8, transwell invasion, wound healing and flow cytometry assays. Besides, we established the CRC tumor models in nude mice to study the effect of SH3PXD2A‐AS1 on the tumor growth. Based on the ceRNA hypothesis, we used miRDB and miRTarBase websites to identify the SH3PXD2A‐AS1‐related ceRNA regulatory network, and measured the roles of this network in CRC cells. The results revealed that the expression profiles of SH3PXD2A‐AS1 from GEO and TCGA databases showed an aberrant high level in CRC tissues compared with colorectal normal tissues. SH3PXD2A‐AS1 over‐expression was also found in CRC cells. SH3PXD2A‐AS1 knockdown inhibited the CRC cellular proliferation, invasion and migration but induced apoptosis. Besides, SH3PXD2A‐AS1 knockdown also suppressed the growth of CRC tumors. Furthermore, SH3PXD2A‐AS1 could function as a ceRNA of miR‐330‐5p. Additionally, UBA2 was proved to be a target gene of miR‐330‐5p. Moreover, SH3PXD2A‐AS1 knockdown downregulated UBA2 expression through sponging miR‐330‐5p to inactivate the Wnt/β‐catenin signaling pathway, thereby inhibiting the cell growth and promoting apoptosis. Therefore, the SH3PXD2A‐AS1/miR‐330‐5p/UBA2 network could regulate the progression of CRC through the Wnt/β‐catenin pathway. These findings offer new sights for understanding the pathogenesis of CRC and provide potential biomarkers for CRC treatment.

Development of epithelial-mesenchymal transition-related lncRNA signature for predicting survival and immune microenvironment in pancreatic cancerwithexperiment validation

“…SH3PXD2A-AS1 was also an antisense transcript transcribed from SH3PXD2A and overexpressed in colorectal cancer. SH3PXD2A‐AS1 acted as an oncogene that could promote cellular proliferation, invasion and migration [ 38 ]. We found it was obvious that the high expression of SH3PXD2A-AS1 is related to the poor prognosis in pancreatic cancer.…”

Long noncoding RNA SH3PXD2A-AS1 promotes NSCLC proliferation and accelerates cell cycle progression by interacting with DHX9

“…SH3PXD2A-AS1 is an antisense transcript transcribed from SH3PXD2A and located on chromosome 10 and is 2023 bp in length. SH3PXD2A-AS1 has recently been reported to be upregulated in colorectal cancer tissues and to promote cell proliferation, cell cycle progression, migration and invasion [ 14 , 15 ]. However, its biological role and specific mechanism in NSCLC should be further uncovered.…”

Long non-coding RNA OTUD6B-AS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells via downregulation of microRNA-3171

“…cancer.gov/) analysis in the UALCAN database (ualcan.path. uab.edu/) (19). A Mann-Whitney test was used to determine the statistical significance of the difference in OTUD6B-AS1 expression between normal and tumor samples.…”

“…Human RNA-sequencing data from colorectal cancer projects, which included 482 patients with CRC and 155 normal tissues (project no. TCGA-COAD) were obtained by TCGA ( ) analysis in the UALCAN database ( ) ( 19 ). A Mann-Whitney test was used to determine the statistical significance of the difference in OTUD6B-AS1 expression between normal and tumor samples.…”