<scp>SLUG</scp> is upregulated and induces epithelial mesenchymal transition in canine oral squamous cell carcinoma

Noguchi, Shunsuke; Hirano, Kazuya; Tanimoto, Nanami; Shimada, Terumasa; Akiyoshi, Hideo

doi:10.1111/vco.12755

Cited by 6 publications

(6 citation statements)

References 22 publications

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“…These results suggest that the investigation of the EMT process in canine oral and cutaneous squamous cell carcinomas may allow a more accurate prediction of their biological behavior [ 285 ]. Another more recent study underlined the importance of SLUG in promoting migration and invasion through EMT induction in a canine oral squamous cell carcinoma cell line [ 286 ].…”

Section: An Overview Of Epithelial-to-mesenchymal Transition and Mese...mentioning

confidence: 99%

An Overview of Epithelial-to-Mesenchymal Transition and Mesenchymal-to-Epithelial Transition in Canine Tumors: How Far Have We Come?

Armando

Mazzola

Ferrari

et al. 2022

Veterinary Sciences

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Historically, pre-clinical and clinical studies in human medicine have provided new insights, pushing forward the contemporary knowledge. The new results represented a motivation for investigators in specific fields of veterinary medicine, who addressed the same research topics from different perspectives in studies based on experimental and spontaneous animal disease models. The study of different pheno-genotypic contexts contributes to the confirmation of translational models of pathologic mechanisms. This review provides an overview of EMT and MET processes in both human and canine species. While human medicine rapidly advances, having a large amount of information available, veterinary medicine is not at the same level. This situation should provide motivation for the veterinary medicine research field, to apply the knowledge on humans to research in pets. By merging the knowledge of these two disciplines, better and faster results can be achieved, thus improving human and canine health.

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Section: An Overview Of Epithelial-to-mesenchymal Transition and Mese...mentioning

confidence: 99%

An Overview of Epithelial-to-Mesenchymal Transition and Mesenchymal-to-Epithelial Transition in Canine Tumors: How Far Have We Come?

Armando

Mazzola

Ferrari

et al. 2022

Veterinary Sciences

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“…This pattern is similar to that observed in Gu et al, where IHC indicated a higher SLUG expression in human breast carcinoma than in normal mammary gland tissue. In terms of oral squamous cell carcinoma (SCC), this pattern is slightly different from that in canine oral SCC studied by Noguchi et al, where SLUG expression is mainly observed in the nucleus and partly in the cytoplasm [ 20 ]. However, in human studies of oral SCC, SLUG expression was detected in 71% of tumor cell cytoplasm [ 15 ].…”

Section: Discussionmentioning

confidence: 91%

“…Moreover, high SLUG expression is associated with poor clinical outcomes in human gastric cancer [ 14 ], esophageal squamous cell carcinoma [ 15 , 16 ], colorectal carcinoma [ 17 ], lung adenocarcinoma [ 18 ], and prostate cancer [ 19 ]. To the best of our knowledge, SLUG has been studied only in association with canine oral squamous cell carcinoma [ 20 ] and melanoma in a mouse model [ 21 ]. Furthermore, human breast cancers are correlated with high SLUG expression.…”

Section: Introductionmentioning

confidence: 99%

Upregulation of SLUG expression in canine mammary gland tumors and its prognostic significance

Cheon

Kim

2023

BMC Vet Res

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Background SLUG (also known as snai2), which is a transcription factor in epithelial–mesenchymal transition (EMT), plays an important role in tumorigenesis. Several human studies have revealed that SLUG expression downregulates E-cadherin activity to induce metastasis and invasion of tumor cells, and its association with tumor mechanisms is under constant evaluation. In clinical veterinary medicine, one study revealed upregulated SLUG expression in canine oral squamous cell carcinoma. However, the association between canine mammary gland tumors (MGT), the most common neoplasm in intact female dogs, and SLUG has not been investigated yet. Therefore, this study aimed to evaluate the differences in SLUG expression among canine normal mammary gland tissue and MGTs using immunohistochemistry. In addition, its prognostic significance was evaluated by analyzing the correlation with the Ki-67 proliferation index and various clinicopathological features. Results SLUG expression increased substantially from normal mammary gland tissues to MGTs, especially showing the strongest expression in malignant MGT than in benign MGT. Negative SLUG expression was observed in mostly normal mammary gland tissues, whereas all tissues in malignant MGT showed positive SLUG expression. Furthermore, positive SLUG expression was associated with higher Ki-67 index, larger tumor size (> 3 cm), and metastasis. Kaplan–Meier survival curve analysis revealed that positive SLUG expression was significantly associated with poor overall and disease-free survival. Conclusions These results indicate that SLUG is upregulated in canine MGTs and positive SLUG expression is positively correlated with poor prognosis. Thus, SLUG protein can be a novel biomarker and therapeutic target for canine patients with MGT.

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“…Validation of the mechanisms involved in TSCC metastasis is required to improve the prognosis. This study revealed that miR-203 is downregulated in both TSCC and NTSCC and targets SLUG , which plays an important role in sustaining the mesenchymal phenotype, resulting in the suppression of the migration and invasion of TSCC cells but not those of oSCC cells ( 5 ).…”

Section: Discussionmentioning

confidence: 95%

“…EMT is regulated by mesenchymal transcription factors, such as zinc finger E-Box Binding Homeobox 1, TWIST, SNAIL, and SLUG ( 4 ). In our previous study, SLUG has been suggested to be upregulated in canine oral SCC and is associated with the migration and invasion of TSCC and NTSCC cells ( 5 ).…”

Section: Introductionmentioning

confidence: 99%

microRNA-203 inhibits migration and invasion of canine tonsillar squamous cell carcinoma cells by targeting SLUG

Noguchi

Matsui²

2023

Front. Vet. Sci.

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ObjectiveSquamous cell carcinoma (SCC) occurring in the tonsils (TSCC) has a poorer prognosis than SCC occurring in other regions of the oral cavity (non-tonsillar SCC [NTSCC]) because it easily metastasizes to distant organs. This study aimed to elucidate the molecular mechanisms underlying the migration and invasion of TSCC cells in vitro.Materials and methodsThis study focused on differential microRNA (miRNA) expression using microRNA microarrays and quantitative polymerase chain reaction in canine TSCC and NTSCC tissues and cell lines. A target gene of the miRNA involved in cell migration and invasion was validated by wound healing, transwell, and luciferase assays.ResultsmiR-203 expression was lower in TSCC tissues than in the normal oral mucosa and NTSCC tissues. Transfection of the miR-203 mimic resulted in the downregulation of mesenchymal marker protein expression and attenuation of cell migration and invasion in TSCC cells, but not in NTSCC cells. A dual-luciferase assay revealed that miR-203 directly targeted the mesenchymal transcription factor SLUG. SLUG overexpression enhances the migration of TSCC cells.ConclusionOur study indicates that the miR-203/SLUG axis may be involved in the metastatic mechanisms of TSCC.

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SLUG is upregulated and induces epithelial mesenchymal transition in canine oral squamous cell carcinoma

Cited by 6 publications

References 22 publications

An Overview of Epithelial-to-Mesenchymal Transition and Mesenchymal-to-Epithelial Transition in Canine Tumors: How Far Have We Come?

An Overview of Epithelial-to-Mesenchymal Transition and Mesenchymal-to-Epithelial Transition in Canine Tumors: How Far Have We Come?

Upregulation of SLUG expression in canine mammary gland tumors and its prognostic significance

microRNA-203 inhibits migration and invasion of canine tonsillar squamous cell carcinoma cells by targeting SLUG

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