<scp>SNARE</scp>Components and Mechanisms of Exocytosis in Plants

Blatt, Michael R.; Thiel, Gerhard

doi:10.1002/9781119312994.apr0089

Cited by 4 publications

(5 citation statements)

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“…The EXO70 family of subunits in land plants shows substantial gene expansion, with 23 paralogues in Arabidopsis (Arabidopsis thaliana) that may reflect specialization in the maintenance of different tethering domains (Synek et al, 2006;Cvrčková et al, 2012). SNARE proteins are classified by the Q or R amino acid residue found at the core of the interacting assembly and localize to vesicle (R-SNAREs or vesicle-associated membrane proteins [VAMPs]) and target (Qa-, Qb-, and Qc-SNAREs) membranes (Fasshauer et al, 1998;Blatt and Thiel, 2003;Jahn and Scheller, 2006). SNARE function is conserved within eukaryotes, but these gene families are similarly expanded in land plants consistent with evolutionary specialization (Sanderfoot et al, 2000;Blatt and Thiel, 2003;Pratelli et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…SNARE proteins are classified by the Q or R amino acid residue found at the core of the interacting assembly and localize to vesicle (R-SNAREs or vesicle-associated membrane proteins [VAMPs]) and target (Qa-, Qb-, and Qc-SNAREs) membranes (Fasshauer et al, 1998;Blatt and Thiel, 2003;Jahn and Scheller, 2006). SNARE function is conserved within eukaryotes, but these gene families are similarly expanded in land plants consistent with evolutionary specialization (Sanderfoot et al, 2000;Blatt and Thiel, 2003;Pratelli et al, 2004). The Qa-SNAREs, SYP121 and SYP122, have overlapping functions at the plasma membrane and are associated with accelerated traffic during growth as well as specialized functions in abiotic and biotic stress resistance (Grefen et al, 2015;Karnik et al, 2017;Waghmare et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Synergy among Exocyst and SNARE Interactions Identifies a Functional Hierarchy in Secretion during Vegetative Growth

et al. 2020

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Vesicle exocytosis underpins signaling and development in plants and is vital for cell expansion. Vesicle tethering and fusion are thought to occur sequentially, with tethering mediated by the exocyst and fusion driven by assembly of soluble NSF attachment protein receptor (SNARE) proteins from the vesicle membrane (R-SNAREs or vesicle-associated membrane proteins [VAMPs]) and the target membrane (Q-SNAREs). Interactions between exocyst and SNARE protein complexes are known, but their functional consequences remain largely unexplored. We now identify a hierarchy of interactions leading to secretion in Arabidopsis (Arabidopsis thaliana). Mating-based split-ubiquitin screens and in vivo Förster resonance energy transfer analyses showed that exocyst EXO70 subunits bind preferentially to cognate plasma membrane SNAREs, notably SYP121 and VAMP721. The exo70A1 mutant affected SNARE distribution and suppressed vesicle traffic similarly to the dominant-negative truncated protein SYP121 DC , which blocks secretion at the plasma membrane. These phenotypes are consistent with the epistasis of exo70A1 in the exo70A1 syp121 double mutant, which shows decreased growth similar to exo70A1 single mutants. However, the exo70A1 vamp721 mutant showed a strong, synergy, suppressing growth and cell expansion beyond the phenotypic sum of the two single mutants. These data are best explained by a hierarchy of SNARE recruitment to the exocyst at the plasma membrane, dominated by the R-SNARE and plausibly with the VAMP721 longin domain as a nexus for binding.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synergy among Exocyst and SNARE Interactions Identifies a Functional Hierarchy in Secretion during Vegetative Growth

et al. 2020

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“…Although considered a housekeeping process, vesicle trafficking also plays an important role in regulation of stress response (Levine et al 2001;Mazel et al 2004). In fact, vesicle transport is important for maintaining homeostasis, cell growth and development, and polarity (Blatt and Thiel 2003). The membrane vesicle transport machinery includes phospholipids and integral membrane proteins, such as vesicle-associated membrane proteins (VAMPs), the latter being the major constituent of soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptors (SNARE) complexes.…”

Section: Introductionmentioning

confidence: 99%

Gene encoding vesicle-associated membrane protein-associated protein from Triticum aestivum (TaVAP) confers tolerance to drought stress

Singh

Khurana

et al. 2018

Cell Stress and Chaperones

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Abiotic stresses like drought, salinity, high and low temperature, and submergence are major factors that limit the crop productivity. Hence, identification of genes associated with stress response in crops is a prerequisite for improving their tolerance to adverse environmental conditions. In an earlier study, we had identified a drought-inducible gene, vesicle-associated membrane protein-associated protein (TaVAP), in developing grains of wheat. In this study, we demonstrate that TaVAP is able to complement yeast and Arabidopsis mutants, which are impaired in their respective orthologs, signifying functional conservation. Constitutive expression of TaVAP in Arabidopsis imparted tolerance to water stress conditions without any apparent yield penalty. Enhanced tolerance to water stress was associated with maintenance of higher relative water content, photosynthetic efficiency, and antioxidant activities. Compared to wild type, the TaVAP-overexpressing plants showed enhanced lateral root proliferation that was attributed to higher endogenous levels of IAA. These studies are the first to demonstrate that TaVAP plays a critical role in growth and development in plants, and is a potential candidate for improving the abiotic stress tolerance in crop plants.

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“…Firstly, vesicles must fuse with target cells, and this process is promoted by a protein called soluble N -ethylmaleimide sensitive factor adaptor protein receptors (SNAREs) ( Gao et al, 2017 ). Associated Sec1/Munc18 (SM) proteins in Arabidopsis were found to involve intimal transport between the ER and the Golgi apparatus ( Blatt and Thiel, 2018 ). Vehicle associated membrane proteins 721 and 722 were involved in the transport of substances necessary for plant growth and defence response ( Yi et al, 2013 ; Yun et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Proteomics and Co-expression Network Analysis Reveal the Importance of Hub Proteins and Metabolic Pathways in Nicotine Synthesis and Accumulation in Tobacco (Nicotiana tabacum L.)

Duan

et al. 2022

Front. Plant Sci.

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Nicotine is a unique alkaloid present in tobacco that is widely used in cigarettes and in the agricultural, chemical, and pharmaceutical industries. However, the research on nicotine is mostly limited to its synthesis pathways, and only a few studies have explored the effects of other metabolic pathways on nicotine precursors. Regulating the nicotine content in tobacco can greatly promoting the application of nicotine in other fields. In this study, we performed global data-independent acquisition proteomics analysis of four tobacco varieties. Of the four varieties, one had high nicotine content and three had a low nicotine content. A total of 31,259 distinct peptides and 6,018 proteins across two samples were identified. A total of 45 differentially expressed proteins (DEPs) co-existed in the three comparison groups and were mainly involved in the transport and metallic processes of the substances. Most DEPs were enriched in the biosynthesis of secondary metals, glutathione metabolism, carbon metabolism, and glycolysis/gluconeogenesis. In addition, the weighted gene co-expression network analysis identified an expression module closely related to the nicotine content (Brown, r = 0.74, P = 0.006). Gene Ontology annotation and Kyoto Encyclopaedia of Genes and Genomes enrichment analysis showed that the module proteins were mainly involved in the synthesis and metabolism of nicotine precursors such as arginine, ornithine aspartate, proline, and glutathione. The increased levels of these precursors lead to the synthesis and accumulation of nicotine in plants. More importantly, these proteins regulate nicotine synthesis by affecting the formation of putrescine, which is the core intermediate product in nicotine anabolism. Our results provide a reference for tobacco variety selection with a suitable nicotine content and regulation of the nicotine content. Additionally, the results highlight the importance of other precursor metabolism in nicotine synthesis.

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SNAREComponents and Mechanisms of Exocytosis in Plants

Abstract: The sections in this article areIntroductionIntegration and Regulation of Secretion in PlantsTemporal and Spatial Analysis of Vesicle FusionSNAREGenomicsConclusions and Future DirectionsAcknowledgements

Cited by 4 publications

References 160 publications

Synergy among Exocyst and SNARE Interactions Identifies a Functional Hierarchy in Secretion during Vegetative Growth

Synergy among Exocyst and SNARE Interactions Identifies a Functional Hierarchy in Secretion during Vegetative Growth

Gene encoding vesicle-associated membrane protein-associated protein from Triticum aestivum (TaVAP) confers tolerance to drought stress

Proteomics and Co-expression Network Analysis Reveal the Importance of Hub Proteins and Metabolic Pathways in Nicotine Synthesis and Accumulation in Tobacco (Nicotiana tabacum L.)

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