<scp>TNF</scp>‐<i>α</i>‐induced <scp>IL</scp>‐6 and <scp>MMP</scp>‐9 expression in immortalized ameloblastoma cell line established by <scp>hTERT</scp>

Ohta, Kouji; Naruse, Takako; Ishida, Yasushi; Shigeishi, Hideo; Nakagawa, Takayuki; Fukui, Akiko; Nishi, Hiromi; Sasaki, Kazuki; Ogawa, Ikuko; Takechi, Masaaki

doi:10.1111/odi.12594

Cited by 25 publications

(22 citation statements)

References 53 publications

(61 reference statements)

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“… 46 , 47 Matrix metalloproteinases (MMPs), also known as matrixins, are calcium-dependent zinc-containing endopeptidases. 48 The MMPs belong to a larger family of proteases known as the metzincin superfamily. 49 Collectively, these enzymes are capable of degrading all kinds of extracellular matrix proteins, but also can process a number of bioactive molecules.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Matrine derivate MASM uncovers a novel function for ribosomal protein S5 in osteoclastogenesis and postmenopausal osteoporosis

et al. 2017

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Postmenopausal osteoporosis (POMP) is a public health problem characterized by decreased bone density and increased fracture risk. Over-activated osteoclastogenesis plays a vital role in POMP. Here we developed a novel bioactive compound MASM (M19) based on sophocarpine. Although it showed no significant effects on osteogenesis and adipogenesis for bone marrow-derived mesenchymal stem cells (BMSCs) in vitro, it could significantly inhibit RANKL/M-CSF induced osteoclastogenesis through suppressing NF-κB, MAPKs and PI3K/Akt pathways in vitro and ameliorate bone loss in ovariectomized mice in vivo. Ribosomal protein s5 (RPS5) has been identified as a target of M19 and regulates PI3K/Akt, NF-κB and MAPKs pathways in osteoclastogenesis. Overexpressions of RPS5 synergistically inhibited osteoclastogenesis with M19 while silencing RPS5 compromised M19 inhibitory effects on osteoclastogenesis in vitro. Among the three pathways, Akt plays a major role in M19 effects. The Akt activator SC79 partially reversed the inhibitory effects on osteoclastogenesis by M19 and RPS5-knocking-down. It indicates that RPS5 serves as a potential candidate target for inhibiting osteoclastogenesis and osteoporosis therapy and M19 is a promising agent for POMP treatment.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Matrine derivate MASM uncovers a novel function for ribosomal protein S5 in osteoclastogenesis and postmenopausal osteoporosis

et al. 2017

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“…MMP series have crucial role in bone destruction, and the difference in expression of these factors could affect the behavior of the tumor, such as invasiveness . Other group reported that TNF‐α induced the expression IL‐6 and MMP‐9 from ameloblastoma cells . However, we have not yet clarified factors that influence the differences in collective cellular invasion in ameloblastoma.…”

Section: Discussionmentioning

confidence: 99%

Fibroblasts promote the collective invasion of ameloblastoma tumor cells in a 3D coculture model

et al. 2017

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Ameloblastoma is a benign tumor of the odontogenic epithelium with several histological subtypes. All subtypes of ameloblastoma contain abundant stroma; the tumor cells invade collectively into the surrounding tissues without losing intratumor cell attachments. However, the molecular mechanisms mediating ameloblastoma invasion remain unclear. Here, we evaluated the functional significance of the interactions between ameloblastoma tumor cells and stromal fibroblasts on collective cellular invasion using a three‐dimensional cultivation method, double‐layered collagen gel hemisphere ( DL ‐ CGH ) culture. The AM ‐1 plexiform and AM ‐3 follicular human ameloblastoma cell lines and HFF ‐2 human fibroblasts were labeled with GFP and DsRed, respectively. Collective cellular invasion of ameloblastoma cells was assessed in the presence or absence of fibroblasts. Notably, without fibroblasts, AM ‐1 cells formed sharp, plexiform‐like invasive processes, whereas AM ‐3 cells formed a series of blunt processes often observed during collective migration. In comparison, under the cocultures with HFF ‐2 fibroblasts, AM ‐3 cells formed tuft‐like invasive processes and collectively invaded into outer layer more than that observed with AM ‐1 cells. Moreover, HFF ‐2 fibroblasts localized to the tips of the invasive tumor processes. These findings suggest that tumor‐associated cells assist tumor cell invasion. Microscopic analysis of sectioned three‐dimensional cultures revealed that AM ‐3/ HFF ‐2 hemispheres were histologically similar to follicular ameloblastoma tumor samples. Therefore, our findings suggest that ameloblastoma subtypes exhibit distinct invasion patterns and that fibroblasts promote collective tumor invasion in follicular ameloblastoma.

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“…In fact, MMPs have been associated with a wide range of inflammatory disorders [18]. MMP upregulation and downregulation in response to inflammatory mediators, such as cytokines and chemokines has been long demonstrated [19,20], but the existence of feedback mechanisms has been highlighted in recent years [9]. Migration of immune cells by processing extracellular matrix; stimulation and cessation of immune-cell recruitment by processing chemokines and other chemotactic molecules; exacerbation and resolution of inflammatory responses by processing complement, cytokines, and other non-matrix bioactive molecules have been reported [9,12,21,22,23,24,25].…”

Section: Introductionmentioning

confidence: 99%

Matrix Metalloproteinases as Regulators of Periodontal Inflammation

Cavalla

Patricia

Sorsa

et al. 2017

IJMS

239

196

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Periodontitis are infectious diseases characterized by immune-mediated destruction of periodontal supporting tissues and tooth loss. Matrix metalloproteinases (MMPs) are key proteases involved in destructive periodontal diseases. The study and interest in MMP has been fuelled by emerging evidence demonstrating the broad spectrum of molecules that can be cleaved by them and the myriad of biological processes that they can potentially regulate. The huge complexity of MMP functions within the ‘protease web’ is crucial for many physiologic and pathologic processes, including immunity, inflammation, bone resorption, and wound healing. Evidence points out that MMPs assemble in activation cascades and besides their classical extracellular matrix substrates, they cleave several signalling molecules—such as cytokines, chemokines, and growth factors, among others—regulating their biological functions and/or bioavailability during periodontal diseases. In this review, we provide an overview of emerging evidence of MMPs as regulators of periodontal inflammation.

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TNF‐α‐induced IL‐6 and MMP‐9 expression in immortalized ameloblastoma cell line established by hTERT

Abstract: We established an immortalized AM cell line by hTERT transfection. TNF-α-mediated regulation of MMP-9 and IL-6 via NF-κB may play an important role in the pathological behaviour of AMs, such as bone resorption.

Cited by 25 publications

References 53 publications

RETRACTED ARTICLE: Matrine derivate MASM uncovers a novel function for ribosomal protein S5 in osteoclastogenesis and postmenopausal osteoporosis

RETRACTED ARTICLE: Matrine derivate MASM uncovers a novel function for ribosomal protein S5 in osteoclastogenesis and postmenopausal osteoporosis

Fibroblasts promote the collective invasion of ameloblastoma tumor cells in a 3D coculture model

Matrix Metalloproteinases as Regulators of Periodontal Inflammation

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