Screen and Study of Tetracycline‐Degrading Bacteria From Activated Sludge and Granular Sludge

Abstract

Wastewater treatment cannot effectively remove tetracycline contained in wastewater, and environmental residues accumulate as a result of this condition. Existing methods use to address the high cost of wastewater treatment and other economic and effective means have been explored. Aerobic granular sludge technology is a newly developed potential biological wastewater treatment method, but the removal of tetracycline through this process is limited. Therefore, the collection of tetracycline‐degrading bacteria as a biological fortifier is vital. In this experiment, 20 and 24 tetracycline‐degrading bacteria are isolated from granular and activated sludge samples, respectively. Highly efficient tetracycline‐degrading strains are selected. The effect of these strains on the degradation rate is investigated under the influence of different factors, namely, time, pH, extra carbon source, inoculum concentration of extra nitrogen source, and substrate concentration. Determination of the nucleotide sequence of the gene encoding for 16S rRNA and physiological and biochemical identification shows that two strains belonged to Achromobacter species.

Microbial Degradation of Tetracycline Antibiotics: Mechanisms and Environmental Implications

“…Achromobacter sp. SA-1-2 isolated from activated sludge showed significantly increased degradation of TC after being cultured at 30 °C, pH 7.0 for 6 days using 3% sodium acetate as an additional carbon source Pseudomonas sp.…”

Isolation, identification, and optimization of conditions for the degradation of four sulfonamide antibiotics and their metabolic pathways in Pseudomonas stutzeri strain DLY-21

“…The degradation of sulfonamide antibiotics by microbes in the environment is a complex and variable process that involves multiple microbial species and degradation mechanisms. There are two main stages of degradation: enzymatic and metabolic pathways [ 14 ]. Enzymatic degradation involves the hydrolysis of the amide bond of sulfonamide antibiotics by specific enzymes, resulting in the production of inactive metabolites.…”

Biological Mitigation of Antibiotic Resistance Gene Dissemination by Antioxidant-Producing Microorganisms in Activated Sludge Systems

“…Municipal wastewater treatment plants (WWTPs) are a major sink of antibiotic residues due to the intersection of multiple antibiotic sources, including hospital, pharmaceutical, and livestock wastewater. − Unfortunately, antibiotics cannot be removed effectively by traditional wastewater treatment methods. Low concentrations of antibiotics pose an ecotoxicological risk to receiving environments. − Moreover, antibiotics at subinhibitory concentrations can induce mutations that confer resistance and HGT of ARGs, both of which drive drug selective pressure and ARG prevalence. − …”