Screen of Chryseobacterium CHANGBAI-2 strain and Identification of the Antifungal Antibiotic from Its Fermentation Broth

Abstract

Abstract. CHANGBAI-2 actinomycete strain was obtained by separating different kinds of soils from the nature. Moreover, the morphology, cultural characteristics, physiological-biochemical characteristics and 16S rDNA sequences of this strain were studied. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the related species and showed 99% identity of nucleotide sequence of 16S rDNA with Chryseobacterium. The antifungal substance produced by CHANGBAI-2 was purified by means of macro-porous adsorbent resin, and the crystal of the antibiotic with high purity was got. In this paper, the methods of purification by adsorbing of microporous adsorbent resin and detection by high performance liquid chromatography with mass spectrum (HPLC-MS) were established. The study would be laid a good foundation for its physical-chemical properties.