Screen-printed amperometric microcell for proline iminopeptidase enzyme activity assay

Nagy, Géza; Gyurcsányi, Róbert E.; Cristalli, Alessandra; Neuman, Michael R.; Lindner, Ernő

doi:10.1016/s0956-5663(00)00081-6

Cited by 10 publications

(4 citation statements)

References 29 publications

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“…Conventional ion-selective electrodes were assembled by incorporating a 7-mm polymeric membrane disk inside a Philips body (Moller Glassbläserei, Zürich, Switzerland). Microfabricated planar sensors were constructed as described previously …”

Section: Methodsmentioning

confidence: 99%

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A Chronoamperometric Method To Estimate Changes in the Membrane Composition of Ion-Selective Membranes

et al. 2001

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A new chronoamperometric method is used to estimate changes in the membrane composition of mobile-site, ionophore-based membranes. The characteristic features of the chronoamperometric curves (initial current, slope, break time) of valinomycin-based, potassium-selective membranes loaded with potassium tetrakis(4-chlorophenyl)borate are correlated with the mobile-site and free ionophore concentration in the membrane. limiting cases for strong and negligible ion pair formation are distinguished. Replicate measurements indicate a relative standard deviation in the calculated values less than 10%. The practical applicability of the method was tested with membranes incorporated into conventional ion-selective electrode bodies or cast onto microfabricated planar sensor structures.

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Section: Methodsmentioning

confidence: 99%

“…Screen-printed planar sensors on a ceramic substrate with a 2-mm-diameter silver disk electrode in a 100-μm-deep well were cleaned with acetone and methanol and allowed to dry. Experimental conditions of screen-printing are described elsewhere …”

Section: Methodsmentioning

confidence: 99%

A Chronoamperometric Method To Estimate Changes in the Membrane Composition of Ion-Selective Membranes

et al. 2001

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“…Microfabricated planar voltammetric cells, similar to the one shown in the detector of Fig. 1, have been applied to measure polyamines and to assess putrescine oxidase (PUO) enzyme activity (85)(86)(87). These sensors might be adapted for polyamine detection in ESRD or for monitoring polyamine clearance during dialysis, through measuring blood or dialysate levels.…”

Section: Detection Of Polyaminesmentioning

confidence: 99%

“…H 2 O 2 is oxidized on a platinum-working electrode at 0.6 V vs. an Ag|AgCl reference electrode. Thus depending on the sensing arrangement, the oxidation current of H 2 O 2 is directly proportional to the diamine concentration or the PUO enzyme activity (85,86).…”

Section: Detection Of Polyaminesmentioning

confidence: 99%

Generation, clearance, toxicity, and monitoring possibilities of unaccounted uremic toxins for improved dialysis prescriptions

Atherton

Bissler

Pendley

et al. 2018

American Journal of Physiology-Renal Physiology

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Current dialysis-dosing calculations provide an incomplete assessment of blood purification. They exclude clearances of protein-bound uremic toxins (PB-UTs), such as polyamines, p-cresol sulfate, and indoxyl sulfate, relying solely on the clearance of urea as a surrogate for all molecules accumulating in patients with end-stage renal disease (ESRD). PB-UTs clear differently in dialysis but also during normal renal function. The kidney clears PB toxins via the process of secretion, whereas it clears urea through filtration. Herein, we review the clearance, accumulation, and toxicity of various UTs. We also suggest possible methods for their monitoring toward the ultimate goal of a more comprehensive dialysis prescription. A more inclusive dialysis prescription would retain the kidney-filtration surrogate, urea, and consider at least one PB toxin as a surrogate for UTs cleared through cellular secretion. A more comprehensive assessment of UTs that includes both secretion and filtration is expected to result in a better understanding of ESRD toxicity and consequently, to reduce ESRD mortality.

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Arch

Doyon

Twede

et al. 2009

The Wiley Encyclopedia of Packaging Technology

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Screen-printed amperometric microcell for proline iminopeptidase enzyme activity assay

Cited by 10 publications

References 29 publications

A Chronoamperometric Method To Estimate Changes in the Membrane Composition of Ion-Selective Membranes

A Chronoamperometric Method To Estimate Changes in the Membrane Composition of Ion-Selective Membranes

Generation, clearance, toxicity, and monitoring possibilities of unaccounted uremic toxins for improved dialysis prescriptions

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