Screen-printed biosensors using different alcohol oxidases

Patel, Narayan G.; Meier, Sebastián; Cammann, Karl; Chemnitius, Gabriele-Christine

doi:10.1016/s0925-4005(01)00545-7

Cited by 89 publications

(64 citation statements)

References 33 publications

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“…At biosensors without MWCNT but with poly(neutral red) mediator [49], a similar reduction is seen. However, the lack of stability of alcohol oxidase is well known [51] and both this study and the results in Ref. [49] represent an improvement with respect to previous results regarding stability.…”

Section: Operational Lifetime and Biosensor Selectivitysupporting

confidence: 53%

Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol

Gouveia‐Caridade

Pauliukaitė

Brett

2008

Electrochimica Acta

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Functionalised multi-walled carbon nanotubes (MWCNTs) were cast on glassy carbon (GC) and carbon film electrodes (CFE), and were characterised electrochemically and applied in a glucose-oxidase-based biosensor. MWCNT-modified carbon film electrodes were then used to develop an alcohol oxidase (AlcOx) biosensor, in which AlcOx-BSA was cross-linked with glutaraldehyde and attached by drop-coating. The experimental conditions, applied potential and pH, for ethanol monitoring were optimised, and ethanol was determined amperometrically at −0.3 V vs. SCE at pH 7.5. Electrocatalytic effects of MWCNT were observed with respect to unmodified carbon film electrodes. The sensitivity obtained was 20 times higher at carbon film/MWCNT-based biosensors than without MWCNT.

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Section: Operational Lifetime and Biosensor Selectivitysupporting

confidence: 53%

Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol

Gouveia‐Caridade

Pauliukaitė

Brett

2008

Electrochimica Acta

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“…The H 2 O 2 , produced by enzymatic reactions, was measured at +200 mV vs. Ag/AgCl; this working potential was chosen as a compromise between high biosensor sensitivity and low current signal due to electrochemical interferences. Other biosensors for the detection of ethanol in wine and beer were developed (Boujtita, Hart, & Pittson, 2000;Patel, Meier, Cammann, & Chemnitius, 2001) using platinum or carbon (doped with cobalt phthalocyanine) screen-printed electrodes and alcohol oxidase. In both cases, the H 2 O 2 , produced by the enzymatic reactions, was measured at high applied potentials and for this reason 0308-8146/$ -see front matter Ó 2011 Elsevier Ltd. All rights reserved.…”

Section: Introductionmentioning

confidence: 99%

Real time monitoring of alcoholic fermentation with low-cost amperometric biosensors

et al. 2011

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a b s t r a c tScreen-printed glucose, ethanol and fructose biosensors, coupled with portable instrumentation, and their application to monitor micro-alcoholic fermentations (micro-ALFs) in red wine is described. For the fabrication of glucose and ethanol biosensors, graphite screen-printed sensors modified with Prussian Blue were coupled with oxidase enzymes while for the fructose biosensor, a bare screen-printed sensor was coated with fructose dehydrogenase and phenazine methansulphate was used as electrochemical mediator. The working range, reproducibility of probe fabrication and biosensor stability were all evaluated. After a recovery study, performed analysing fortified must-wine samples, the biosensors were employed to monitor micro-ALFs induced by the inoculation of two different strains of Saccharomyces cerevisiae. During the red micro-ALFs, samples of must-wine were collected and analysed by use of both biosensors and spectrophotometric kits. The data obtained demonstrated that a biosensor-based system could represent a useful tool to assist winemakers during wine production.

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“…Classical methods such as refractometry (Pen'kovskii et al, 2004) or densitometry (Bavcar & Kosmerl, 2003) of the distilled samples, as well as gas chromatography (Schmitt & Aderjan, 2004) which are routinely used in in clinics and industry are time-and labour-consuming procedures. In order to replace these methods, a variety of enzymatic and biosensor approaches were developed based on alcohol-specific enzymes isolated from different microbial species: NAD + -dependent alcohol dehydrogenase (Ivanova et al, 2003;Gautier et al, 1990), quinohemoprotein alcohol dehydrogenase (Niculescu et al, 2002), or alcohol oxidase (Gibson et al, 1992;Gonchar et al, 2001Patel et al, 2001) were proposed and applied for the determination of ethanol in complex samples. Alcohol biosensors based on NAD + -dependent alcohol dehydrogenase (ADH) usually exhibit high selectivity, however, due to the need to add the coenzyme NAD + to the sample, ethanol determination is quite expensive using these types of biosensors.…”

Section: Development Of a Reagentless Bienzyme Amperometric Biosensormentioning

confidence: 99%

Amperometric Biosensors for Lactate, Alcohols, and Glycerol Assays in Clinical Diagnostics

Smutok¹,

Gayda²,

Dmytruk³

et al. 2011

Biosensors - Emerging Materials and Applications

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Screen-printed biosensors using different alcohol oxidases

Cited by 89 publications

References 33 publications

Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol

Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol

Real time monitoring of alcoholic fermentation with low-cost amperometric biosensors

Amperometric Biosensors for Lactate, Alcohols, and Glycerol Assays in Clinical Diagnostics

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