Screen‐Printed Carbon Electrodes Modified with Cellobiose Dehydrogenase: Amplification Factor for Catechol vs. Reversibility of Ferricyanide

Dock, Eva; Ruzgas, Tautgirdas

doi:10.1002/elan.200390059

Cited by 16 publications

(8 citation statements)

References 22 publications

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“…[5b-e, 6,81] What is possibly unique for CDH in relation to the other enzymes used is that CDH has a much better long-term stability than the others mentioned above and it also seems to be unaffected by the electrochemical recycling process, in which radicals are prone to be formed, possibly reflecting its natural role as radical scavenger as proposed in Scheme 1.…”

Section: Biosensorsmentioning

confidence: 95%

Cellobiose Dehydrogenase: A Versatile Catalyst for Electrochemical Applications

et al. 2010

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Cellobiose dehydrogenase catalyses the oxidation of aldoses--a simple reaction, a boring enzyme? No, neither for the envisaged bioelectrochemical applications nor mechanistically. The catalytic cycle of this flavocytochrome is complex and modulated by its flexible cytochrome domain, which acts as a built-in redox mediator. This intramolecular electron transfer is modulated by the pH, an adaptation to the environmental conditions encountered or created by the enzyme-producing fungi. The cytochrome domain forms the base from which electrons can jump to large terminal electron acceptors, such as redox proteins, and also enables by that path direct electron transfer from the catalytically active flavodehydrogenase domain to electrode surfaces. The application of electrochemical techniques to the elucidation of the molecular and catalytic properties of cellobiose dehydrogenase is discussed and compared to biochemical methods. The results lead to valuable insights into the function of this cellulose-bound enzyme, but also form the basis of exciting applications in biosensors, biofuel cells and bioelectrocatalysis.

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Section: Biosensorsmentioning

confidence: 95%

Cellobiose Dehydrogenase: A Versatile Catalyst for Electrochemical Applications

et al. 2010

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“…Despite the cost of purifi cation, isolation and extraction, enzyme electrodes are still popular in the study of clinically relevant biological processes and the determination of environmental chemical contaminants, pesticides and herbicides (Dock and Ruzgas 2003 ;Albareda-Servent et al 2000 ). Due to their fast response and specifi city towards biorecognition of their substrates, enzyme modifi cation continues to play a role in biosensor design.…”

Section: Introductionmentioning

confidence: 99%

Carbon Nanotubes: Advances, Integration and Applications to Printable Electrode-Based Biosensors

Hung

Kerman

2015

Nanobiosensors and Nanobioanalyses

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The development of novel analytical techniques for biosensor application has been an area of intense research efforts in the past decade and, in a fi eld where the demand for rapid and cost-effective analyses is high, the use of screen-printed electrodes as opposed to conventional macroelectrodes can effectively satisfy these demands. With advancements in nanotechnology, the use of nanomaterials in biosensors has also added another level of customizability and sensitivity. In this chapter, we described the use of carbon nanotubes in printable electrode-based biosensors for label-free detection approaches for the detection of environmental contaminants and biologically relevant substrates. A discussion of past developments and recent advancements will highlight the broad range of experimental designs suitable for biosensing applications. With continual innovation in this fi eld, it is expected that screen-printing technology will come to the fore in biosensing technology and progress successfully to overcome fundamental challenges in the biosensor fi eld.

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“…Electrochemical based pretreatments employ the polarization of the carbon WE in the oxidation range of potentials in different solutions. The use of 0.05 M PBS solutions [38], saturated Na 2 CO 3 solutions [39,40], 0.5 M NaOH solutions [41], 0.5 M H 2 SO 4 solutions (cyclic voltammetry) [40], or 0.1 M H 2 SO 4 (+ 5 μA for 6 min) [42]. The efficiency of these treatments was demonstrated to remove constituents organic from ink [24], to increase the surface roughness [41] or even to increase the number of chemical functionalities [41], resulting in improved kinetics of heterogeneous charge transfer.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Polyphenols in Wine by Voltammetric Techniques with Screen Printed Carbon Electrodes

et al. 2019

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The electrochemical oxidation processes that occur during a voltammetric assay in wine samples lead to the formation of species that obstructs the surface and reduce their active area. This effect is critical for screen printed carbon electrodes (SPCE) and leads to abnormal low values of the total polyphenols content of wines, ca. 72 % lower than those obtained with glassy carbon electrodes. This effect was examined using 10 red and Port wine samples. Mechanical polishing and electrochemical‐based treatments for the removal of this fouling layer were tested. The best results were obtained by electrochemical activation in at a constant potential of 1.2 V during 100 s Na2CO3 saturated solution, and by polishing. The success of some of these treatments brings an added value to SPCE, as it opens the possibility of their reuse in the wine analysis. This outcome is particularly relevant for quality control where a huge number of analysis is performed and the reduction of cost may dictates the choice of the analytical method.

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Screen‐Printed Carbon Electrodes Modified with Cellobiose Dehydrogenase: Amplification Factor for Catechol vs. Reversibility of Ferricyanide

Cited by 16 publications

References 22 publications

Cellobiose Dehydrogenase: A Versatile Catalyst for Electrochemical Applications

Cellobiose Dehydrogenase: A Versatile Catalyst for Electrochemical Applications

Carbon Nanotubes: Advances, Integration and Applications to Printable Electrode-Based Biosensors

Evaluation of Polyphenols in Wine by Voltammetric Techniques with Screen Printed Carbon Electrodes

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