Screen-Printed Electrodes: New Tools for Developing Microbial Electrochemistry at Microscale Level

Estevez-Canales, Marta; Berná, Antonio; Borjas, Zulema; Esteve‐Núñez, Abraham

doi:10.3390/en81112366

Cited by 15 publications

(10 citation statements)

References 51 publications

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“…Among electroactive bacteria, G. sulfurreducens has become a model system for the study of all kinds of METs (Yates et al ., ; Yu et al ., ; Dantas et al ., ; Borjas et al ., ; Estevez‐Canales et al ., ,b) in order to achieve a deeper understanding of the biological mechanisms involved in direct extracellular electron transfer (DEET; Busalmen et al ., ; Lovley, ; Esteve‐Núñez et al ., ; Bond et al ., ; Bonanni et al ., ). In long‐term experiments, with no terminal electron acceptor available apart from the electrode, G. sulfurreducens forms a biofilm on the electrode, reaching a thickness of tens of microns (Snider et al ., ; Schrott et al ., ; Stephen et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Silica immobilization of Geobacter sulfurreducens for constructing ready‐to‐use artificial bioelectrodes

Estevez-Canales

Pinto

Coradin

et al. 2017

Microbial Biotechnology

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SummaryMicrobial electrochemical technologies (METs) rely on the control of interactions between microorganisms and electronic devices, enabling to transform chemical energy into electricity. We report a new approach to construct ready‐to‐use artificial bioelectrodes by immobilizing Geobacter sulfurreducens cells in composite materials associating silica gel and carbon felt fibres. Viability test confirmed that the majority of bacteria (ca. 70 ± 5%) survived the encapsulation process in silica and that cell density did not increase in 96 h. The double entrapment within the silica–carbon composite prevented bacterial release from the electrode but allowed a suitable mass transport (ca. 5 min after electron donor pulse), making the electrochemical characterization of the system possible. The artificial bioelectrodes were evaluated in three‐electrode reactors and the maximum current displayed was ca. 220 and 150 μA cm−3 using acetate and lactate as electron donors respectively. Cyclic voltammetry of acetate‐fed bioelectrodes revealed a sigmoidal catalytic oxidation wave, typical of more advanced‐stage biofilms. The presence of G. sulfurreducens within composites was ascertained by SEM analysis, suggesting that only part of the bacterial population was in direct contact with the carbon fibres. Preliminary analyses of the transcriptomic response of immobilized G. sulfurreducens enlightened that encapsulation mainly induces an osmotic stress to the cells. Therefore, ready‐to‐use artificial bioelectrodes represent a versatile time‐ and cost‐saving strategy for microbial electrochemical systems.

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Section: Introductionmentioning

confidence: 99%

Silica immobilization of Geobacter sulfurreducens for constructing ready‐to‐use artificial bioelectrodes

Estevez-Canales

Pinto

Coradin

et al. 2017

Microbial Biotechnology

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“…The developed microbiosensor can be used to measure acetate concentrations either in bulk solutions or inside biofilms in situ . For example, acetate is consumed or produced during anaerobic conversion in wastewater treatment and in anaerobic digestion processes (Estevez-Canales et al, 2015; Henze, 2008). In addition, there is a need to measure acetate concentrations in the polyphosphate-accumulating biofilms that use acetate as the main electron donor in biological phosphorus removal processes (Meyer et al, 2002; Sudiana et al, 1999).…”

Section: Resultsmentioning

confidence: 99%

Microbiosensor for the detection of acetate in electrode-respiring biofilms

Atçı

Babauta

Sultana

et al. 2016

Biosensors and Bioelectronics

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The goal of this work was to develop a microbiosensor to measure acetate concentration profiles inside biofilms in situ. The working principle of the microbiosensor was based on the correlation between the acetate concentration and the current generated during acetate oxidation by Geobacter sulfurreducens. The microbiosensor consisted of a 30-μm carbon microelectrode with an open tip as a working electrode, with G. sulfurreducens biofilm on the tip and a pseudo Ag/AgCl reference electrode, all enclosed in a glass outer case with a 30-μm tip diameter. The microbiosensor showed a linear response in the 0–1.6 mM acetate concentration range with a 79 ± 8 μM limit of detection (S/N=2). We quantified the stirring effect and found it negligible. However, the interfering effect of alternative electron donors (lactate, formate, pyruvate, or hydrogen) was found to be significant. The usefulness of the acetate microbiosensor was demonstrated by measuring acetate concentration depth profiles within a G. sulfurreducens biofilm. The acetate concentration remained at bulk values throughout the biofilm when no current was passed, but it decreased from the bulk values to below the detection limit within 200 μm when current was allowed to pass. The zero acetate concentration at the bottom of the biofilm showed that the biofilm was acetate-limited.

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“…This confusion could occur, for example, since the cyclic voltammetry (CV) of a model electroactive bacterium like Geobacter sulfurreducens ( Fig. 2 ) [9] , presents oxidation and reduction peaks in a potential range very close to those shown occurring with the copper wire CV ( Fig. 3 A).…”

Section: Methods Validationmentioning

confidence: 99%

Simultaneous characterization of porous and non-porous electrodes in microbial electrochemical systems

et al. 2020

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Adequate electrochemical characterization of electrode material/biofilms is crucial for a comprehensive understanding and comparative performance of bioelectrochemical systems (BES). However, their responses are greatly affected by the metabolic activity and growth of these living entities and/or the interference of electrode wiring that can act as an electroactive surface for growth or constitute a source of contamination by corrosion. This restricts the meaningful comparison of the performance of distinct electrode materials in BES. This work describes a methodology for simultaneous electrochemical control and measurement of the microbial response on different electrode materials under the same physicochemical and biological conditions. The method is based on the use of a single channel potentiostat and one counter and reference electrodes to simultaneously polarize several electrode materials in a sole bioelectrochemical cell. Furthermore, various strategies to minimize wiring corrosion are proposed. The proposed methodology, then, will enable a more rigorous characterization of microbial electrochemical responses for comparisons purposes. Experimental Set-up allows to polarize several working electrodes at the same time. Chronoamperometry can be performed simultaneously with a potentiostat. The physicochemical and biological conditions in each working electrode will be exactly the same

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Screen-Printed Electrodes: New Tools for Developing Microbial Electrochemistry at Microscale Level

Cited by 15 publications

References 51 publications

Silica immobilization of Geobacter sulfurreducens for constructing ready‐to‐use artificial bioelectrodes

Silica immobilization of Geobacter sulfurreducens for constructing ready‐to‐use artificial bioelectrodes

Microbiosensor for the detection of acetate in electrode-respiring biofilms

Simultaneous characterization of porous and non-porous electrodes in microbial electrochemical systems

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