Screening and activity identification of an anti-idiotype nanobody for Bt Cry1F toxin from the camelid naive antibody phage display library

Hao, Jia; Wang, Jingxuan; Xu, Chongxin; Gao, Meijing; Chen, Wei; Zhang, Xiao; Hu, Xiaodan; Liu, Yuan; Liu, Xianjin

doi:10.1080/09540105.2019.1691156

Cited by 6 publications

(4 citation statements)

References 30 publications

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“…Ab2 has the ability to simulate the spatial structure and biological activity of antigens. 65 It can specifically bind with idiotypic antibodies. Ab2 can be generally divided into Ab2α, Ab2β, Ab2γ, and Ab2ε.…”

Section: Mimotopesmentioning

confidence: 99%

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Mimotope-Based Immunoassays for the Rapid Analysis of Mycotoxin: A Review

Huang

et al. 2021

J. Agric. Food Chem.

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Mycotoxins are toxic contaminants in foods and feeds that are naturally occurring and largely unavoidable. Determining their contents in these products is essential to protect humans from harm. Immunoassays of mycotoxins have been well-established because they are fast, sensitive, simple, and cost-effective. However, a major limitation of immunoassays is the requirement of toxic mycotoxins as competing antigens, standards, or competing tracers. Mimotopes are peptides or proteins that can specifically bind to antibodies and compete with analytes for binding sites by mimicking antigenic epitopes. They can be employed as substitutes for competing antigens, standards, or competing tracers to avoid use of mycotoxins. This review summarizes the production and functionalization of the two main kinds of mimotopes, mimic peptides and anti-idiotypic antibodies (Ab2), and their applications in rapid analysis of mycotoxins.

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Section: Mimotopesmentioning

confidence: 99%

“…The concept of anti-idiotype antibodies (Ab2) was first proposed by Jerne as part of his “immune network theory” in 1974. Ab2 has the ability to simulate the spatial structure and biological activity of antigens . It can specifically bind with idiotypic antibodies.…”

Section: Preparation and Functionalization Of Mimotopesmentioning

confidence: 99%

Mimotope-Based Immunoassays for the Rapid Analysis of Mycotoxin: A Review

Huang

et al. 2021

J. Agric. Food Chem.

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“…In recent years, non-toxic immunoassays for mycotoxins are gaining more attention. With the β-type anti-idiotype antibodies bearing the special internal image as substitutes of antigen, it makes the immunoassays for mycotoxins safer and cheaper. − Until now, non-toxic immunoassays for some mycotoxins such as aflatoxin, fumonisin, citrinin, and Bacillus thuringiensis toxins have been successively reported. − Considering the deficiency of our previous studies, to further establish non-toxic immunoassays for TeA mycotoxin is meaningful.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Non-Toxic Immunodetection of Alternaria Mycotoxin Tenuazonic Acid Based on Ferritin-Displayed Anti-Idiotypic Nanobody-Nanoluciferase Multimers

Wang

Wan

et al. 2021

J. Agric. Food Chem.

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The non-toxic immunoassay for mycotoxins is being paid more attention due to its advantages of higher safety and cost savings by using anti-idiotype antibodies to substitute toxins. In this study, with tenuazonic acid (TeA), a kind of highly toxic Alternaria mycotoxin as the target, an enhanced non-toxic immunoassay was developed based on the ferritin-displayed anti-idiotypic nanobody-nanoluciferase multimers. First, three specific β-type anti-idiotype nanobodies (AId-Nbs) bearing the internal image of TeA mycotoxin were selected from an immune phage display library. Then, the AId-Nb 2D with the best performance was exploited to generate a nanoluciferase (Nluc)-functionalized fusion monomer, by which a one-step non-toxic immunodetection format for TeA was established and proven to be effective. To further improve the affinity of the monomer, a ferritin display strategy was used to prepare 2D-Nluc fusion multimers. Finally, an enhanced bioluminescent enzyme immunoassay (BLEIA) was established in which the half maximal inhibitory concentration (IC50) for TeA was 6.5 ng/mL with a 10.5-fold improvement of the 2D-based enzyme-linked immunosorbent assay (ELISA). The proposed assay exhibited high selectivities and good recoveries of 80.0–95.2%. The generated AId-Nb and ferritin-displayed AId-Nb-Nluc multimers were successfully extended to the application of TeA in food samples. This study brings a new strategy for production of multivalent AId-Nbs and non-toxic immunoassays for trace toxic contaminants.

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“…Thus, in comparison with immunoassays based on traditional chemically synthesized antigens, novel developed detection methods employing biosynthetic antigens of VHHs display preferable cost advantages and product stability, so they are suitable for commercial production and widespread use. Accordingly, several anti-idiotypic VHHs (AI-VHHs), including those for mycotoxin zearalenone, fumonisin B 1 , aflatoxin B 1 , Ochratoxin A, deoxynivalenol (DON), and other toxins, have been developed as toxin surrogates in immunoassays (Hao et al, 2020;Liu et al, 2018;Qiu et al, 2016;Shu et al, 2019;Wang et al, 2013;Wang et al, 2016;Zhang et al, 2019). However, these determination methods for mycotoxin surveillance only avoid the application one of the chemically synthetized mycotoxin conjugates or mycotoxin detection standard.…”

Section: Introductionmentioning

confidence: 99%

Anti-idiotypic VHH mediated environmentally friendly immunoassay for citrinin without mycotoxin

Xiong

Zhang

et al. 2020

Food and Agricultural Immunology

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Competitive enzyme-linked immunosorbent assay (ELISA) is the most widely used method for rapidly screening of mycotoxins in contaminated food products; however, it involves the use of toxin-protein conjugates as a competitive antigen and toxin standards as calibrators. In this work, a single domain heavy chain antibody (VHH)-based environmentally friendly ELISA (VHH-ELISA) was developed to measure citrinin concentration in Monascusfermented rice. With this novel indirect competitive VHH-ELISA, the use of a synthetic antigen and pure toxins for operation and environmental safety simultaneously avoided. The assay exhibited a limit of detection of 2.25 ng/mL, with a linear range of 5.0 −500.0 ng/mL. VHH-ELISA was then utilized for sample testing, and excellent agreement was observed between VHH-ELISA and high-performance liquid chromatography as a reference. This study provided a novel platform to develop environmentally friendly immunoassays for other toxic small molecules in the application of anti-idiotypic VHHs.

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Screening and activity identification of an anti-idiotype nanobody for Bt Cry1F toxin from the camelid naive antibody phage display library

Cited by 6 publications

References 30 publications

Mimotope-Based Immunoassays for the Rapid Analysis of Mycotoxin: A Review

Mimotope-Based Immunoassays for the Rapid Analysis of Mycotoxin: A Review

Enhanced Non-Toxic Immunodetection of Alternaria Mycotoxin Tenuazonic Acid Based on Ferritin-Displayed Anti-Idiotypic Nanobody-Nanoluciferase Multimers

Anti-idiotypic VHH mediated environmentally friendly immunoassay for citrinin without mycotoxin

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