Screening and catalytic activity in organic synthesis of novel fungal and yeast lipases

Cardenas, F.; Álvarez, Emilio; Castro-Alvarez, Maria-Soledad de; Sánchez-Montero, J.M.; Valmaseda, M.; Elson, Stephen W.; Sinisterra, J. V.

doi:10.1016/s1381-1177(00)00244-7

Cited by 91 publications

(71 citation statements)

References 39 publications

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“…After autoclaving the medium (121 ºC, 20 min) the mixture was emulsified by vigorous shaking for 15 min, and after cooling to approximately 60 ºC, the medium was transferred to Petri dishes and rapidly cooled. The agar concentration used for the tributyrine assay is in the range suggested by Lawrence (under 1.5%), 29 assuring adequate protein diffusion into the solid medium.…”

Section: Hydrolytic Assaysmentioning

confidence: 99%

“…23,26,29,32 The initial selection for the detection of a particular activity has a significant weighting in the screening process, since it aims to eliminate the worst candidates and, in general, is carried out through the cultivation of microorganisms in a solid culture medium, in Petri dishes. 44 Triacylglycerides such as triolein or -butyrin are used as standard substrates for the determination of lipase activity.…”

Section: Hydrolytic Screeningmentioning

confidence: 99%

“…4 Tributyrine is the substrate which has been most used for detection of lipolytic activity. 23,29,32,45 On being hydrolyzed, tributyrine generates dibutyrine, monoburytine, glycerol and butyric acid, all of which are soluble in water, facilitating the visualization of the translucent halo. 32 Despite the drawbacks associated with the use of tributyrine (since it is not a specific substrate for lipases as it can also be hydrolyzed by esterases), this is a convenient substrate, since it can be dispersed in water by agitation, without the need to add emulsifiers.…”

Section: Hydrolytic Screeningmentioning

confidence: 99%

“…Thus, synthetic screening is fundamental to evaluate the potential application of mycelium-bound lipases. 23,29 Purified commercial enzymes, although widely available, are not selected specifically for catalytic reactions in non-aqueous environments. Thus, synthetic screening in organic media is an alterative which allows the selection of enzymes with specific characteristics for the reaction of interest.…”

Section: Synthetic Screeningmentioning

confidence: 99%

“…16,28 The access to a wide range of lipase types has made them even more desirable for two additional reasons: (i) from the large number of lipases cited in the literature, only the enzymes of a few species have been shown to be stable and have biosynthetic capacities suitable for routine use in organic reactions; and (ii) many important problems remain unsolved in the application of lipases in certain industrial processes. 29 Considering the need for production processes which are more committed to the ideal of sustainability, this study aims to contribute to the broadening of knowledge regarding the biotechnological potential of fungi from the Amazon region, particularly those isolated from flora, as potential mycelium bound-lipase sources for application in the field of biocatalysis. In this study, lipase-producing Amazonian fungi were tested for both hydrolytic and synthetic activities.…”

Section: Introductionmentioning

confidence: 99%

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Potential application in biocatalysis of mycelium-bound lipases from Amazonian fungi

Zanotto¹,

Romano

Duvoisin

et al. 2009

J. Braz. Chem. Soc.

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Neste trabalho foram avaliados 212 fungos isolados de plantas da Região Amazônica quanto ao potencial de aplicação em biocatálise como fornecedores de lipases ligadas ao micélio. Estes isolados foram submetidos a ensaios de atividade hidrolítica e sintética. Quando submetidos ao ensaio com o substrato tributirina, 87% dos isolados apresentaram atividade hidrolítica. Destes, 30% mostraram bom crescimento em meio líquido indutor de lipase, e foram submetidos à avaliação sintética em reações de esterificação e transesterificação em solvente orgânico. Os nove fungos que apresentaram os melhores desempenhos sintéticos foram avaliados quanto a sua enantiosseletividade na reação de resolução do (R,S)-2-octanol. O isolado UEA_115 foi o biocatalisador mais versátil, apresentando bom desempenho nas reações de esterificação (conversão > 90%) e boa capacidade para a resolução do (R,S)-2-octanol (ee s 29%; ee p 99%; c 22%; E > 200). Com este estudo foi possível demonstrar o grande potencial dos fungos amazônicos como fornecedores de lipases para biocatálise.In this study, 212 fungi were isolated from Amazon region plants, aiming to obtain mycelium bound-lipase-producing biocatalysts. These isolates were submitted to hydrolytic and synthetic activity assays. When submitted to the tributyrine substrate test, 87% of the isolates showed hydrolytic activity. Of these, 30% showed good growth in lipase inducing liquid media and were submitted to evaluation of synthetic activity in esterification and transesterification reactions in organic solvents. The nine fungi which had the best synthetic activity were evaluated in the (R,S)-2-octanol resolution reaction, in order to verify the enantioselectivity of mycelium-bound lipases. The isolate UEA_115 was the most versatile biocatalyst, showing good performance in esterification reactions (conversion > 90%) and good ability for the resolution of (R,S)-2-octanol (ee s 29%; ee p 99%; c 22%; E > 200). Thus, this study has demonstrated the great potential of the Amazonian fungi as lipase suppliers for biocatalysts. Keywords: fungi, Amazon region, mycelium-bound lipase, biocatalyst screening, biocatalysis IntroductionIn recent years, global concern regarding the impact of human actions on the environment has been growing. The implementation of the concept of sustainable development in several productive activities has become an urgent issue. Fortunately, biotechnology also offers an increasing potential to meet the global demands for processes and products in greater harmony with the sustainability concept. 1,2 In this regard, one of the most promising tools in biotechnology is the field of applied biocatalysis, also referred to by some authors as biotransformation or enzymatic technology. 2,3 The advantages of using enzymes as biocatalysts in industrial processes have been shown to be ever more evident. Great versatility of catalyzed reactions, mild reaction conditions, and regio, chemo and enantioselective natures are those most frequently mentioned in the literature. 4 Such characteristic...

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Section: Hydrolytic Assaysmentioning

confidence: 99%

Section: Hydrolytic Screeningmentioning

confidence: 99%

Section: Hydrolytic Screeningmentioning

confidence: 99%

Section: Synthetic Screeningmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Potential application in biocatalysis of mycelium-bound lipases from Amazonian fungi

Zanotto¹,

Romano

Duvoisin

et al. 2009

J. Braz. Chem. Soc.

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Preparation and Catalytic Properties of Lipase Immobilized on Epoxy‐functionalized Starch Nanoparticles

Wang

Dong

et al. 2023

ChemistrySelect

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As a natural macromolecular material, starch is an ideal carrier for enzyme immobilization because of its widely available source, easy regeneration and excellent biodegradability. However, the natural starch cannot be directly used for immobilization due to the large particle size and excessive hydrophilicity. In this paper, the epoxy groups were grafted onto esterified starch by bifunctional reagents. The grafting efficiency of epoxy groups was increased by starvation drop addition. The changes of contact angle and particle size are conducive to the adsorption of immobilized enzyme at the water oil interface in the subsequent enzyme catalytic reaction, hence improve of the lipase activity. The maximum amount of lipase mobilized on such modified starch was ∼143.7 mg/g, which was near 40 mg/g higher than that of those from direct crosslinking. The immobilized enzymes showed excellent resistance to organic solvents and good reusability. Immobilization of lipases on epoxy‐functionalized starch nanoparticles can potentially improves the possibility of enzymes industrial application.

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Current Awareness

2002

Yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 14th. Nov. 2001)

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Screening and catalytic activity in organic synthesis of novel fungal and yeast lipases

Cited by 91 publications

References 39 publications

Potential application in biocatalysis of mycelium-bound lipases from Amazonian fungi

Potential application in biocatalysis of mycelium-bound lipases from Amazonian fungi

Preparation and Catalytic Properties of Lipase Immobilized on Epoxy‐functionalized Starch Nanoparticles

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