Screening and characterization of biosurfactant produced by Pseudoxanthomonas sp. G3 and its applicability for enhanced oil recovery

Abstract

Biosurfactants are one of the microbial bioproducts that are in most demand from microbial-enhanced oil recovery (MEOR). We isolated and screened potential biosurfactant-producing bacteria, followed by biosurfactant production and characterization, and a simulation of the MEOR application to biosurfactants in a sand-packed column. Isolate screening was conducted based on qualitative (hemolytic blood assay and oil-spreading test) and semi-qualitative (emulsification assay and interfacial tension measurement) parameters. Bacterial identification was performed using 16S rRNA phylogenetic analysis. Sequential isolation yielded 32 bacterial isolates, where Pseudomonas sp. G3 was able to produce the most biosurfactant. Pseudomonas sp. G3 had the highest emulsification activity (Ei = 72.90%) in light crude oil and could reduce the interfacial tension between oil and water from 12.6 to 9.7 dyne/cm with an effective critical-micelle concentration of 0.73 g/L. The Fourier transform infrared spectrum revealed that the biosurfactant produced was a glycolipid compound. A stable emulsion of crude extract and biosurfactant formed at pH 2-12, up to 100 °C, and with a NaCl concentration of up to 10% (w/v) in the response-surface method, based on the Box-Behnken design model. The sand-packed column experiment with biosurfactant resulted in 20% additional oil recovery. Therefore, this bacterium and its biosurfactant show potential and the bacterium is suitable for use in MEOR applications.

Biosurfactant production by Trichoderma sp. MK116452 and its possible application in oil recovery

“…There are several reports highlighting the success of ex situ biosurfactant-mediated MEOR at the laboratory scale, which highlights the ease of application and high success rate (Qazi et al 2013;Kanna 2017;Ashish and Debnath (Das) 2018;Santos et al 2018;Ali et al 2019;Astuti et al 2019;Câmara et al 2019;de Araujo et al 2019;Ghazala et al 2019;Maia et al 2019). Biosurfactants can recover around 10-85% of oil from sand pack columns or core floods.…”

“…Biosurfactant-mediated MEOR is based on the production of biosurfactants by specific microorganisms (Luna et al 2016;Kanna 2017;de Araujo et al 2019). Biosurfactants are an attractive option for oil recovery as they have several advantages over chemical surfactants, such as low critical micelle concentration, high biodegradability, greater stability under extreme conditions, and low toxicity (Ashish and Debnath (Das) 2018; Astuti et al 2019;Maia et al 2019). Biosurfactant use in oil recovery reduces the interfacial tension between oil and water and promotes the formation of micelles, which provides a physical mechanism for mobilizing oil with a moving aqueous phase, thereby releasing trapped residual oil from reservoirs (Kanna 2017;Maia et al 2019).…”

Biosurfactant production by Trichoderma sp. MK116452 and its possible application in oil recovery

“…There were numerous articles emphasizing the achievement of ex situ biosurfactant-mediated MEOR at the laboratory scale, which highlights the comfort of application and huge success rate (Qazi et al 2013;Kanna 2017;Ashish and Debnath (Das) 2018;Santos et al 2018;Ali et al 2019;Astuti et al 2019;Câmara et al 2019;de Araujo et al 2019;Ghazala et al 2019;Maia et al 2019).…”

“…Biosurfactant-mediated MEOR is the use of biosurfactant produced by specific microorganisms to enhance oil recovery (Luna et al 2016;Kanna 2017;de Araujo et al 2019). Biosurfactants have several advantages over chemical surfactants such as low critical micelle concentration, high biodegradability, greater stability under extreme conditions, and low toxicity which make them a better choice for oil recovery (Ashish and Debnath (Das) 2018; Astuti et al 2019;Maia et al 2019). In oil recovery, biosurfactant indorse the formation of micelles and lower the interfacial tension between oil and water, which gives a physical mechanism for mobilizing oil with a moving aqueous phase, thereby the trapped residual oil from reservoirs are released (Kanna 2017;Maia et al 2019).…”

Research on the Influence of Different Temperature Conditions on the Microbial Community in Anearobic-Anoxic-Oxic process on the Plateau

“…Pseudoxanthomonas from γ-proteobacteria dominated the Pseudoxanthomonas genus (> 99%) with little change in abundance under four temperature conditions. As reported in many experiments, Pseudoxanthomonas has better degradation ability compared with common non-steroidal anti-inflammatory drugs (NSAIDs), such as Diclofenac (DCF), Ibuprofen (IBU), and Naproxen (NAP) [39], and has higher Biosurfactants production potential [40].…”