Screening and characterization of phosphate solubilizing bacteria from isolate of thermophilic bacteria

Yulianti, Evy; Rakhmawati, Anna

doi:10.1063/1.4995207

Cited by 11 publications

(11 citation statements)

References 3 publications

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“…For instance, bioavailability of phosphate in soil is critical for plant growth due to its role in morphological and physiological functions such as energy storage and transfer, cell division, root elongation, and photosynthesis, etc. (Yulianti and Rakhmawati, 2017). However, available forms of phosphate even in fertile soil is generally low at sub-micromolar levels (Arif et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

16S rRNA Gene Amplicon Based Metagenomic Signatures of Rhizobiome Community in Rice Field During Various Growth Stages

et al. 2019

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Rice is a major staple food across the globe. Its growth and productivity is highly dependent on the rhizobiome where crosstalk takes place between plant and the microbial community. Such interactions lead to selective enrichment of plant beneficial microbes which ultimately defines the crop health and productivity. In this study, rhizobiome modulation is documented throughout the development of rice plant. Based on 16S rRNA gene affiliation at genus level, abundance, and diversity of plant growth promoting bacteria increased during the growth stages. The observed α diversity and rhizobiome complexity increased significantly (p < 0.05) during plantation. PCoA indicates that different geographical locations shared similar rhizobiome diversity but exerted differential enrichment (p < 0.001). Diversity of enriched genera represented a sigmoid curve and subsequently declined after harvest. A major proportion of dominant enriched genera (p < 0.05, abundance > 0.1%), based on 16S rRNA gene, were plant growth promoting bacteria that produces siderophore, indole-3-acetic acid, aminocyclopropane-1-carboxylic acid, and antimicrobials. Hydrogenotrophic methanogens dominated throughout cultivation. Type I methanotrophs (n = 12) had higher diversity than type II methanotrophs (n = 6). However, the later had significantly higher abundance (p = 0.003). Strong enrichment pattern was also observed in type I methanotrophs being enriched during water logged stages. Ammonia oxidizing Archaea were several folds more abundant than ammonia oxidizing bacteria. K-strategists Nitrosospira and Nitrospira dominated ammonia and nitrite oxidizing bacteria, respectively. The study clarifies the modulation of rhizobiome according to the rice developmental stages, thereby opening up the possibilities of bio-fertilizer treatment based on each cultivation stages.

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Section: Discussionmentioning

confidence: 99%

16S rRNA Gene Amplicon Based Metagenomic Signatures of Rhizobiome Community in Rice Field During Various Growth Stages

et al. 2019

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“…Pikovskaya's selective medium for screening the phosphate solubilizing bacteria employed in the present study [11,12]. Plates were inoculated with sterile root segments and incubated at 28°C for 7 days.…”

Section: Growing the Bacteria In Selective Mediamentioning

confidence: 99%

Endophytic bacterial metagenomics and phosphate solubilization activities in an endemic legume Humboldtia brunonis Wall.

Shendye¹,

Thamizhseran²

2022

J App Biol biotech

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Phosphate's abiotic stress and bioavailability limit its availability to plants. Plants collaborate with phosphorus solubilizing microorganisms to resolve this issue, resulting in a distinct microbial ecology. Bacterial diversity and other functional attributes of plant-associated microbial ecology in the endemic legume Humboldtia brunonis Wall. have been studied using 16s metagenomic amplicon analysis and culture-dependent selective media-based culture techniques. This study aimed to gain insight into bacterial diversity and isolate phosphate solubilizing bacteria. Bacterial isolates were grown in Pikovskaya's medium and incubated at a required temperature for 48 h before getting characterized by examining colony morphology with physiological and biochemical properties. The pure culture of bacterium is identified and assigned to its taxonomy using 16S rRNA gene sequence analysis as a tool. Brevibacillus brevis is the phosphate solubilizing endophyte associated with this endemic plant. A clear zone encircling the bacterial colony on Pikovskaya's medium indicates that this bacterium can solubilize phosphate. To better apprehend the endophytic root bacterial diversity, Illumina MiSeq metagenomics using the GAIA workflow, discovered forty-three endophytic bacterial species. The use of METAGENassist and KEGG database examined taxonomic and functional attributes of the endophytes. Exploration of biofertilizing, biocontrolling, and phytostimulating abilities is evident by the presence of the bacteria contributing to dinitrogen and nitrogen fixers, carbon fixers, ammonia oxidizers, and nitrite reducers. The figure also emphasizes their energy sources, oxygen requirement, sporulation, and their nature of interaction with the plant. The endophyte community also delivers information on this endemic plant's survival strategy.

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“…One promising solution is to create environmentally friendly bio-fertilizers containing PGPB. Phosphorus is the second most important nutrient, after nitrogen, for plants but is typically insoluble in the soil ( 15 ). For plant roots to take up phosphate, insoluble inorganic phosphate needs to be converted to soluble phosphate ( 15 , 16 ).…”

Section: Introductionmentioning

confidence: 99%

Plant Growth-Promoting Activity of Bacteria Isolated from Asian Rice (Oryza sativa L.) Depends on Rice Genotype

et al. 2022

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Screening and characterization of phosphate solubilizing bacteria from isolate of thermophilic bacteria

Cited by 11 publications

References 3 publications

16S rRNA Gene Amplicon Based Metagenomic Signatures of Rhizobiome Community in Rice Field During Various Growth Stages

16S rRNA Gene Amplicon Based Metagenomic Signatures of Rhizobiome Community in Rice Field During Various Growth Stages

Endophytic bacterial metagenomics and phosphate solubilization activities in an endemic legume Humboldtia brunonis Wall.

Plant Growth-Promoting Activity of Bacteria Isolated from Asian Rice (Oryza sativa L.) Depends on Rice Genotype

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