Screening and confirmation of steroids and nitroimidazoles in urine, blood, and food matrices: Sample preparation methods and liquid chromatography tandem mass spectrometric separations

Tölgyesi, Ádám; Barta, Enikő; Simon, Ágnes; McDonald, Thomas J.; Sharma, Virender K.

doi:10.1016/j.jpba.2017.08.005

Cited by 20 publications

(8 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this way, it could be observed that the optimized method is simpler than other works reported in the literature. For instance, it did not require the use of the SPE techniques, − ,− ,, which are related to time-consuming experiments that demand great amounts of organic solvents for the conditioning and washing steps. In addition, the developed sample extraction protocol did not use any chemical derivatization reaction, which usually leads to more expensive and longer procedures. ,− …”

Section: Resultsmentioning

confidence: 99%

“…Liquid chromatography (LC) coupled with mass spectrometry (MS) is frequently employed due to the vast working concentration ranges and to the wide variety of compounds to be investigated. − Gas chromatography (GC) coupled with MS has also been employed; ,, however, an additional derivatization step for non-volatile compounds is frequently required. Low-resolution mass spectrometry (LRMS) has accounted for most of the works regarding multiresidue methods due to the higher sensitivity and instrumental robustness. − ,− The use of high-resolution mass spectrometry (HRMS), especially the hybrid spectrometers with a combination between LRMS and HRMS, has been increasingly studied, e.g. Q-Orbitrap, a quadrupole-orbitrap hybrid mass spectrometer. ,, …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Quantitative and Confirmatory Method Employing Liquid Chromatography Coupled to Hybrid High-Resolution Mass Spectrometry and QuEChERS for the Determination of Thirty-Seven Growth Promoter Residues in Bovine Urine

Silva

Borgati²,

Rocha³

et al. 2021

J. Agric. Food Chem.

View full text Add to dashboard Cite

In this work, 37 growth promoters were quantitatively determined in bovine urine using a QuEChERS approach with acetonitrile, NaCl, and MgSO4:PSA for sample extraction. The analytes were separated and detected by liquid chromatography coupled to hybrid high-resolution mass spectrometry. The method was validated in accordance with the Decision 657/2002/EC guidelines, in which recoveries fell within the range 84–113%, relative standard varied between 2 and 32%, and detection limit between 0.1 and 2.5 μg L–1. An adequate performance was evidenced during a proficiency test evaluation, and the developed method has been applied to routine analysis of growth promoters in Brazil. A highlight is the easiness of sample extraction combined with a quantitative determination of forbidden drugs using high-resolution mass spectrometry, which enables retrospective analysis in a surveillance perspective.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Quantitative and Confirmatory Method Employing Liquid Chromatography Coupled to Hybrid High-Resolution Mass Spectrometry and QuEChERS for the Determination of Thirty-Seven Growth Promoter Residues in Bovine Urine

Silva

Borgati²,

Rocha³

et al. 2021

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Tube-based liquid-liquid extraction has proven to be largely manual, mainly because of the need for pipetting of low-viscosity high-vapor-pressure solvents. However, supported liquid extraction media of both natural and synthetic origins have been introduced to provide a sorbent for the liquid-liquid extraction process to occur in an SPE-like manner [ 74 - 76 ]. Novel pipetting systems have been developed to support formats typical for liquid handling footprints [ 77 , 78 ].…”

Section: Sample Extractionmentioning

confidence: 99%

Review of the Use of Liquid Chromatography-Tandem Mass Spectrometry in Clinical Laboratories: Part I-Development

Rappold

2022

Ann Lab Med

View full text Add to dashboard Cite

The process of method development for a diagnostic assay based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) involves several disparate technologies and specialties. Additionally, method development details are typically not disclosed in journal publications. Method developers may need to search widely for pertinent information on their assay(s). This review summarizes the current practices and procedures in method development. Additionally, it probes aspects of method development that are generally not discussed, such as how exactly to calibrate an assay or where to place quality controls, using examples from the literature. This review intends to provide a comprehensive resource and induce critical thinking around the experiments for and execution of developing a clinically meaningful LC-MS/MS assay.

show abstract

“…Sample preparation is an essential component of MS analysis of food and biological matrices. To date, there have been many clean-up techniques used in the sample preparation of urine, blood [33,34], and food matrices [35][36][37], including SPE, SPME, liquid-liquid extraction, and QuEChERS methods. As an easy-to-use sample preparation, QuECh-ERS is used to clean up the drugs in the sample in this study.…”

Section: Extraction Condition Optimizationmentioning

confidence: 99%

Rapid screening for 315 drugs in food and biological matrices by ultrahigh‐performance liquid chromatography quadrupole time of flight mass spectrometry and its application to a specific incident

Guo

Yang

Zhang

2022

Separation Science Plus

View full text Add to dashboard Cite

Comprehensive screening of harmful chemical drugs in food is an essential analytical tool in the investigation of food poisoning. Meanwhile, determining harmful chemical drugs in biological matrices is a supplement to support the screening results in food. In this study, we developed a screening method for 315 harmful chemical drugs in food and biological matrices based on ultrahigh-performance liquid chromatography quadrupole time of flight mass spectrometry. The food and gastric juice sample was extracted by 1.0% formic acid acetonitrile and acetonitrile, respectively, and cleaned up by a C18 sorbent. Urine and serum were directly extracted by 1.0 % formic acid acetonitrile. Then the data was acquired and screened for drugs using a self-built library. The recovery, accuracy, and limit of quantitation for methodology were investigated. The method was validated by other three laboratories in different areas in China using different mass spectrometric techniques. For screening, the coefficient of variation of the retention times was below 5%. The recoveries for the drugs in nine matrices were 70-120%.At last, the developed method was applied to a real incident, demonstrating its suitability for the sensitive and fast screening of harmful chemical drugs in food and biological samples.

show abstract

Screening and confirmation of steroids and nitroimidazoles in urine, blood, and food matrices: Sample preparation methods and liquid chromatography tandem mass spectrometric separations

Cited by 20 publications

References 24 publications

A Quantitative and Confirmatory Method Employing Liquid Chromatography Coupled to Hybrid High-Resolution Mass Spectrometry and QuEChERS for the Determination of Thirty-Seven Growth Promoter Residues in Bovine Urine

A Quantitative and Confirmatory Method Employing Liquid Chromatography Coupled to Hybrid High-Resolution Mass Spectrometry and QuEChERS for the Determination of Thirty-Seven Growth Promoter Residues in Bovine Urine

Review of the Use of Liquid Chromatography-Tandem Mass Spectrometry in Clinical Laboratories: Part I-Development

Rapid screening for 315 drugs in food and biological matrices by ultrahigh‐performance liquid chromatography quadrupole time of flight mass spectrometry and its application to a specific incident

Contact Info

Product

Resources

About