Screening and confirmatory methods for the determination of melamine in cow’s milk and milk-based powdered infant formula: Validation and proficiency-tests of ELISA, HPLC-UV, GC-MS and LC-MS/MS

Lutter, Petra; Savoy-Perroud, Marie-Claude; Campos-Giménez, Esther; Meyer, Liliane; Goldmann, Till; Bertholet, M.-C.; Mottier, Pascal; Desmarchelier, Aurélien; Monard, Florence; Perrin, C.; Robert, Fabien; Delatour, Thierry

doi:10.1016/j.foodcont.2010.11.022

Cited by 113 publications

(50 citation statements)

References 15 publications

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“…In addition to retention time confirmation, the spectrometric profile of MEL recorded between 200 and 400 nm has been used to confirm the identity of a selected peak. However, the detection limit is around the parts per million (ppm) level (Kim et al, 2008;Muniz-Valencia et al, 2008;Chou et al, 2003;Lutter et al, 2011;Venkatasami & Sowa, 2010). Gas chromatography (GC), and HPLC coupled with single stage mass spectrometry (MS) or tandem mass spectrometry (MS/MS) are frequently used for the analysis of MEL and related compounds.…”

Section: Selective Quantitative Methodsmentioning

confidence: 99%

“…The selected ion monitoring mode (SIM) was used for quantification and full scan mode for further identification (Litzau et at, 2008). LC-MS/MS has many applications in MEL analysis in food, feed and urine (Andersen et al, 2008;Somker & Krynitsky, 2008;Cheng et al, 2009a;Ibáñez et al, 2009;Zhang et al, 2010;Lutter et al, 2011) LC is suitable for the separation of polar MEL, and double stage mass (MS/MS) analysis provides high selectivity and sensitivity, with a reported detection limit range from 0.01 to 0.1 µg/g.…”

Section: Selective Quantitative Methodsmentioning

confidence: 99%

“…Retention times are prolonged for polar compounds and sensitivity is enhanced in mass spectrometry. Other methods use reversed-phase C18 or C8 columns (Lin et al, 2008;Venkatasami & Sowa Jr, 2010;Ibánez et al, 2009;Lutter et al, 2011). Recently, UPLC has been introduced in this field.…”

Section: Chromatographic and Mass Analysis (Ionization Fragmentationmentioning

confidence: 99%

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Application of Tandem Mass Spectrometry for Analyzing Melamine

Cheng¹,

Wang²

2012

Tandem Mass Spectrometry - Applications and Principles

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Section: Selective Quantitative Methodsmentioning

confidence: 99%

Section: Selective Quantitative Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Application of Tandem Mass Spectrometry for Analyzing Melamine

Cheng¹,

Wang²

2012

Tandem Mass Spectrometry - Applications and Principles

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“…Though, many detection methods for MEL have been developed. Currently, standard analytical techniques for determination of melamine are either low cost, low sensitivity techniques such as enzyme linked immunosorbent assays (ELISA) 8 and high performance liquid chromatography with spectrophotometric diode array detector (HPLC-UV-DAD), 9 or high sensitivity, high cost as chromatography-tandem mass spectrometry (GC-MS/MS) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) 10,11 hyphenated techniques. Hence, alternative methods include electrospray ionization mass spectrometry (ESI-MS), 12 ion-pair liquid chromatography-tandem mass spectrometry, 13 electromembrane-LPME followed by HPLC, 14 gas chromatography/mass spectrometry, 11 high-field asymmetric ion mobility spectrometry combined with solid-phase J. Braz.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Highly Sensitive Determination of Melamine in Different Food Samples by Corona Discharge Ion Mobility Spectrometry after Dispersive Liquid-Liquid Microextraction

Mirzajani¹,

Tavaf²

2016

Journal of the Brazilian Chemical Society

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In this work, for the first time, a highly sensitive method based on dispersive liquid-liquid microextraction coupled with positive corona discharge ion mobility spectrometry as a fast and inexpensive technique has been evaluated for the direct determination of melamine. The effective parameters influencing the microextraction efficiency, such as pH, the properties of extraction and dispersion solvent were investigated. Under the optimum conditions, analytical parameters such as linearity, precision and limit of detection were evaluated. The calibration graph of melamine determination using the proposed method has two regions of linearity from 0.5 to 70 ng mL -1 and 70 to 1500 ng mL -1 , the limit of detection and limit of quantification are 0.25 and 0.5 ng mL -1 , respectively, and the relative standard deviation was less than 3% for all experiments. The proposed method was successfully applied for the determination of melamine in the milk, dairy products and egg yolk.

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“…Melamine, chemically known as 2,4,6-triamino-1,3,5-triazine, is produced in large amounts (1.2 million tons in 2007) primarily for use in the synthesis of melamine formaldehyde resins for the fabrication of laminates, plastics coatings, commercial filters, glues or adhesives, as well as for dishware and kitchenware [1]. Melamine contamination has been reported in products such as milk, infant formula, frozen yogurt, pet food, biscuits, candy, and coffee drinks.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Molecularly Imprinted Polymer as SPE Sorbent for Melamine Isolation

et al. 2013

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Abstract:In this paper, a separation procedure combining molecularly imprinted-solid phase extraction (MI-SPE) was developed for the isolation of melamine. The molecularly imprinted polymer (MIP) was prepared using precipitation polymerization method where melamine as template, 9-vinylcarbazole as functional monomer, ethtylene glycol dimethacrylate as a cross-linker and benzoyl peroxide as initiator. An off-line MI-SPE method followed by ultra-performance liquid chromatography detection of melamine was established. MIP showed a better affinity toward melamine compared to non imprinted polymer (NIP) with a maximum binding capacity of 53.01 mg/g MIP. Based on the correlation coefficients, the kinetic study indicated that the adsorption of melamine by MIP fit a pseudo-second order model. From isotherm study, adsorption of melamine by MIP increased when the concentration of melamine increased and followed a Freundlich isotherm model, which indicates the sorption can be described by multilayer sorption. The interference study proved that MIP has better binding capacity towards melamine if compared to NIP due to specific sites of melamine occurred in MIP particles.

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Screening and confirmatory methods for the determination of melamine in cow’s milk and milk-based powdered infant formula: Validation and proficiency-tests of ELISA, HPLC-UV, GC-MS and LC-MS/MS

Cited by 113 publications

References 15 publications

Application of Tandem Mass Spectrometry for Analyzing Melamine

Application of Tandem Mass Spectrometry for Analyzing Melamine

Rapid and Highly Sensitive Determination of Melamine in Different Food Samples by Corona Discharge Ion Mobility Spectrometry after Dispersive Liquid-Liquid Microextraction

Preparation and Characterization of Molecularly Imprinted Polymer as SPE Sorbent for Melamine Isolation

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