Screening and Evaluation of Human Intestinal Lactobacilli for the Development of Novel Gastrointestinal Probiotics

Abstract

The aim of this study was to screen intestinal lactobacilli strains for their advantageous properties to select those that could be used for the development of novel gastrointestinal probiotics. Ninety-three isolates were subjected to screening procedures. Fifty-nine percent of the examined lactobacilli showed the ability to auto-aggregate, 97% tolerated a high concentration of bile (2% w/v), 50% survived for 4 h at pH 3.0, and all strains were unaffected by a high concentration of pancreatin (0.5% w/v). One Lactobacillus buchneri strain was resistant to tetracycline. None of the tested strains caused lysis of human erythrocytes. Six potential probiotic strains were selected for safety evaluation in a mouse model. Five of 6 strains caused no translocation, and were considered safe. In conclusion, several strains belonging to different species and fermentation groups were found that have properties required for a potential probiotic strain. This study was the first phase of a multi-phase study aimed to develop a novel, safe and efficient prophylactic and therapeutic treatment system against gastrointestinal infections using genetically modified probiotic lactobacilli.

Screening and characterization of new potentially probiotic lactobacilli from breast-fed healthy babies in Pakistan

“…There have been a number of reports for isolating the probiotic lactobacilli from human gastrointestinal tract (Dunne et al, 2001;Koll et al, 2010;Morelli, 2000); none is from healthy babies in Pakistan. With different environments, social set up and immunity in Pakistani babies, we aimed to isolate, identify and characterize probiotic lactobacilli.…”

Evaluation of Survival, Free Radical Scavenging and Human Enterocyte Adherence Potential of Lactobacilli with Anti-Obesity and Anti-Inflammatory CLA Isomer-Producing Attributes

“…The results are not unexpected as lactobacilli more often derived from intestinal sources were well adaptable to acid and bile conditions (Kõll et al . ). The suggested reason favored that this strain might have better H+‐ATPase and Bsh activity (Matsumoto et al .…”

Screening and evaluation of Lactobacillus spp. for the development of potential probiotics

“…The Lactobacillus was categorized on the basis of fermentation group and physiological properties of isolates. The fermentation of glucose without gas, growth at 37°C and no growth at 15°C identifies obligately homofermentative lactobacilli (OHOL); growth both at 15 and 37°C without gas production is characteristic of facultatively heterofermentative lactobacilli (FHEL), whereas gas production at 37°C and variable growth at 15°C are characteristic of obligately heterofermentative lactobacilli (OHEL) (Koll et al, 2010).…”

“…The effect of pancreatin on the survival of lactobacilli was examined by inoculating 1% 24 h old culture in MRS broth containing pancreatin (0.5% w/v) followed by incubation at 37°C and 200 rpm for 24-48 h (Koll et al, 2010). Growth was measured by taking OD after a time interval of 24 and 48 h at 620 nm (Systronics, Japan).…”