Screening and evaluation of phenols and furans degrading fungi for the biological pretreatment of lignocellulosic biomass

A Lignocellulolytic Colletotrichum sp. OH with Broad-Spectrum Tolerance to Lignocellulosic Pretreatment Compounds and Derivatives and the Efficiency to Produce Hydrogen Peroxide and 5-Hydroxymethylfurfural Tolerant Cellulases

“…As only handful of mixed inhibitor tolerant fungi are reported (reviewed in [11,16]), new and novel fungal isolates from diverse ecosystems for improved bioabatement of inhibitors are desirable [25]. Moreover, most studies test low concentrations of the inhibitors or focus only on detoxification or its associated mechanisms [11,16].…”

“…To do this, we tested the concentrated crude extract from the rice husk-grown cultures for the activities of six enzymes, endoglucanase, BGL, endoxylanase, Lac, Lip, and MnP. The enzyme assays consisted of all sixteen inhibitor combinations (Table 1 (25,50, and 75 mM) were used to test the enzyme activities. Thus, for each enzyme, the activities were tested in 16 combinations with three IC values (48 reactions) and relative activities were compared for tolerance (Figure 8A-F).…”

“…Biological detoxification is an economical and ecologically friendly method well integrated with bioprocesses, such as separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and consolidated bioprocessing (CBP) [23,24]. Tolerance and detoxification of harmful pretreatment-derived inhibitors using microorganisms such as bacteria, filamentous fungus, and yeast are well researched and documented [11,16,25]. Among them, filamentous fungi are considered as a robust platform in lignocellulosic bioprocesses because of several adaptations such as high and stable secretion of tolerant enzymes [12], enhanced production of multiple lignocellulolytic enzymes using cheap carbon sources [26,27], and seamless integration into SHF, SSF, and CB processes [28,29].…”

Evaluation of fungal consortium lignozyme for biodelignification of agricultural residues

“…Several pretreatment methods are being used for lignocellulose biomass, including physical, chemical, biological, and physico‐chemical techniques 3 . To prevent the production of fermentation inhibitors such as furfural and phenolic aldehydes, which are produced by other lignocellulosic pretreatments, biologically mediated biomass conversion is more promising 4,5 . Various microorganisms such as bacteria and fungi (soft, brown, and white rot) have the ability to delignify lignocellulosics.…”

“…3 To prevent the production of fermentation inhibitors such as furfural and phenolic aldehydes, which are produced by other lignocellulosic pretreatments, biologically mediated biomass conversion is more promising. 4,5 Various microorganisms such as bacteria and fungi (soft, brown, and white rot) have the ability to delignify lignocellulosics. However, white-rot fungi (WRF) due to their ligninolytic enzyme machinery, have been observed to be the most potent microorganisms to fractionate the lignocellulosic biomass components.…”