Screening and Evaluation of Purine-Nucleoside-Degrading Lactic Acid Bacteria Isolated from Winemaking Byproducts In Vitro and Their Uric Acid-Lowering Effects In Vivo

Abstract

In Taiwan, adult hyperuricemia affects as many as 1 in 4 males and 1 in 6 females, who are predominantly young adults aged 19–45. In this study, lactic acid bacteria (LAB) with acid tolerance, bile salt tolerance and high affinity to intestinal cells were extracted from the side products of alcohol fermentation (distillers’ grains). These bacteria were evaluated for their ability to lower uric acid levels. Qualitative identification and quantitative analysis were performed using high-performance liquid chromatography (HPLC) on the purine-degrading enzymes to select purine-decomposing LAB for animal testing. When the final concentration of purine compounds reached 0.1% and 1%, seven strains of LAB showed potential in degrading purine compounds. HPLC was used to analyze their purine-degrading abilities, and the three best performing LAB strains, (107) 8–16, (107) tau 1–3, and (107) 6–10 were screened for further animal testing with Wistar rats. By the third week, the results showed that strain (107) 6–10 could prevent formation and reduce the levels of blood urea nitrogen (BUN) in yeast extract/potassium oxonate-induced hyperuricemia. The multi-strain lactic acid bacteria (MLAB) performed best for uric acid reduction in the serum and down regulated BUN. Yeast extract/potassium oxonate-induced hyperuricemia had no impact on serum creatinine, while LAB did not affect the creatinine concentration. In summary, MLAB not only protects kidney function but is also effective in regulating uric acid concentration in the body. Hence, MLAB can be used as a functional food supplement that prevents or aids the treatment of hyperuricemia in a rodent model.

A randomized pilot trial assessing the reduction of gout episodes in hyperuricemic patients by oral administration of Ligilactobacillus salivarius CECT 30632, a strain with the ability to degrade purines

“…The nucleoside and/or the purine base, once inside the bacterial cell, enter the salvage pathway for nucleotide synthesis or are degraded to purine metabolites ( Kilstrup et al, 2005 ). Nucleoside transport has been proven in many different species of lactic acid bacteria ( Li et al, 2014 ; Hsieh et al, 2021 ; Lee et al, 2022 ), but the incorporation of inosine and guanosine by L. salivarius strains of human origin is described for the first time in this study. Additionally, it shows that whole cells of 5 selected L. salivarius strains also uptake uric acid, although to a lesser extent than nucleosides.…”

Gut Microbiome and Metabolome Variations in Self-Identified Muscle Builders Who Report Using Protein Supplements

“…More changes were seen in the Firmicutes phyla, and many species in this phylum are butyrate-producing [ 37 ]. The Lactobacillales abundance was significantly greater in the PS group, and this family of bacteria functions in purine absorption [ 39 ] and uric acid decomposition [ 40 ].…”

Evaluation of purine-nucleoside degrading ability andin vivouric acid lowering ofStreptococcus thermophilus(ID-PDP3), a novel antiuricemia strain

“…Lactiplantibacillus pentosus P2020 has been reported to reduce renal inflammation by inhibiting inflammation-related signals and to reduce uric acid levels by affecting the expression of proteins involved in uric acid transport [13]. Altogether, these studies demonstrate that probiotics hold promise as an alternative adjuvant treatment for hyperuricemia to improve renal and intestinal function [13,14].…”