Screening and Evaluation of Yeast Antagonists for Biological Control of<i>Botrytis cinerea</i>on Strawberry Fruits

Chen, Pei Hua; Chen, Rou Yun; Chou, Jui Yu

doi:10.1080/12298093.2018.1454013

Cited by 103 publications

(74 citation statements)

References 52 publications

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“…However, A. subglaciale (AS14) that showed the highest efficacy in reducing B. cinerea colony growth in in vitro assay, performed worse than A. pullulans in vivo. This phenomenon is not uncommon and was also observed in various previous studies (Chen et al 2018 ). Conversely, Di Francesco et al ( 2015 ) showed how A. pullulans strains L1 and L8 were more effective in the control of B. cinerea on apple than in inhibiting fungal conidial germination, displaying a reduction of the pathogen lesion diameter of 88.9% and 94.4% respectively.…”

Section: Discussionsupporting

confidence: 83%

Bioactivity of volatile organic compounds by Aureobasidium species against gray mold of tomato and table grape

Francesco

Zajc

Gunde‐Cimerman

et al. 2020

World J Microbiol Biotechnol

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Aureobasidium strains isolated from diverse unconventional environments belonging to the species A. pullulans, A. melanogenum, and A. subglaciale were evaluated for Volatile Organic Compounds (VOCs) production as a part of their modes of action against Botrytis cinerea of tomato and table grape. By in vitro assay, VOCs generated by the antagonists belonging to the species A. subglaciale showed the highest inhibition percentage of the pathogen mycelial growth (65.4%). In vivo tests were conducted with tomatoes and grapes artificially inoculated with B. cinerea conidial suspension, and exposed to VOCs emitted by the most efficient antagonists of each species (AP1, AM10, AS14) showing that VOCs of AP1 (A. pullulans) reduced the incidence by 67%, partially confirmed by the in vitro results. Conversely, on table grape, VOCs produced by all the strains did not control the fungal incidence but were only reducing the infection severity (< 44.4% by A. pullulans; < 30.5% by A. melanogenum, and A. subglaciale). Solid-phase microextraction (SPME) and subsequent gas chromatography coupled to mass spectrometry identified ethanol, 3-methyl-1-butanol, 2-methyl-1-propanol as the most produced VOCs. However, there were differences in the amounts of produced VOCs as well as in their repertoire. The EC50 values of VOCs for reduction of mycelial growth of B. cinerea uncovered 3-methyl-1-butanol as the most effective compound. The study demonstrated that the production and the efficacy of VOCs by Aureobasidium could be directly related to the specific species and pathosystem and uncovers new possibilities for searching more efficient VOCs producing strains in unconventional habitats other than plants.

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Section: Discussionsupporting

confidence: 83%

Bioactivity of volatile organic compounds by Aureobasidium species against gray mold of tomato and table grape

Francesco

Zajc

Gunde‐Cimerman

et al. 2020

World J Microbiol Biotechnol

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“…Unanimously, studies performed so far recommend VOCs application under air-tight environment, in order to rapidly saturate the atmosphere with the volatile antimicrobials. Particularly effective application of VOCs is reported in the control of storage pathogens on fresh fruit (e.g., citrus, peach, strawberry) but also other commodities such as nuts, grains, and seeds (Table 1; Strobel, 2006;Fialho et al, 2011;Wang et al, 2013;Chen et al, 2018;Gao et al, 2018). Bacteria produce volatile metabolites as part of their normal metabolism ( Figure 1A).…”

Section: Volatile Organic Compounds Of Microbial Originmentioning

confidence: 99%

Scent of a Killer: Microbial Volatilome and Its Role in the Biological Control of Plant Pathogens

2020

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The use of synthetic fungicides represents the most common strategy to control plant pathogens. Excessive and/or long-term distribution of chemicals is responsible for increased levels of environmental pollution, as well as adverse health consequence to humans and animals. These issues are deeply influencing public perception, as reflected by the increasing demand for safer and eco-friendly agricultural commodities and their by-products. A steadily increasing number of research efforts is now devoted to explore the use of safer and innovative approaches to control plant pathogens. The use of microorganisms as biological control agents (BCAs) represents one of the most durable and promising strategies. Among the panoply of microbial mechanisms exerted by BCAs, the production of volatile organic compounds (VOCs) represents an intriguing issue, mostly exploitable in circumstances where a direct contact between the pathogen and its antagonist is not practicable. VOCs are potentially produced by all living microorganisms, and may be active in the biocontrol of phytopathogenic oomycetes, fungi, and bacteria by means of antimicrobial activity and/or other cross-talk interactions. Their biological effects, the reduced residuals in the environment and on agricultural commodities, and the ease of application in different agricultural systems make the use of VOCs a promising and sustainable approach to replace synthetic fungicides in the control of plant pathogens. In this review, we focus on VOCs produced by bacteria and fungi and on their role in the cross-talk existing between the plant pathogens and their host. Biologic systemic effect of the microbial volatile blends on both pathogen and host plant cells is also briefly reviewed.

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“…The mycelium discs of both fungi were placed 6 cm apart on culture medium in opposite sides of the plate. In the control, B. cinerea mycelium was inoculated in both sides of the plates [ 15 ]. After 10 days of incubation in the dark at 22 °C, the radial mycelial growth of B. cinerea towards the endophytic fungus (Ri) and radial mycelial growth of B. cinerea on a control plate (Rc) were measured and the percentage of inhibition was calculated according to the formula (Rc−Ri)/Rc × 100.…”

Section: Methodsmentioning

confidence: 99%

Endophytic Fungi Isolated from Plants Growing in Central Andean Precordillera of Chile with Antifungal Activity against Botrytis cinerea

Vidal¹,

Parada²,

Mendoza³

et al. 2020

JoF

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Botrytis cinerea is an important phytopathogenic fungus affecting the fruit production around the world. This fungus is controlled mainly by using synthetic fungicides, but many resistant isolates have been selected by the indiscriminate use of fungicides. Endophytic fungi or secondary metabolites obtained from them become an alternative method of control for this fungus. The aim of this work was to identify endophytic fungi with antifungal activity against the plant pathogenic fungus B. cinerea isolated from plants from Central Andean Precordillera of Chile. Three endophytic fungi (Ac1, Lc1 and Ec1) with antifungal activity against B. cinerea were isolated from native and endemic plants growing in Central Andean Precordillera of Chile. The isolates Lc1 (isolated from Lithraea caustica) and Ac1 (isolated from Acacia caven) were identified as Alternaria spp. and the isolate Ec1 (isolated from Echinopsis chiloensis) was identified as Aureobasidium spp. The isolated endophytic fungi would inhibit B. cinerea through the secretion of diffusible and volatile compounds affecting the mycelial growth, conidia germination and interestingly, it was also shown that the volatile compounds produced by the three isolated endophytic fungi suppressed the sporulation of B. cinerea.

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Screening and Evaluation of Yeast Antagonists for Biological Control ofBotrytis cinereaon Strawberry Fruits

Cited by 103 publications

References 52 publications

Bioactivity of volatile organic compounds by Aureobasidium species against gray mold of tomato and table grape

Bioactivity of volatile organic compounds by Aureobasidium species against gray mold of tomato and table grape

Scent of a Killer: Microbial Volatilome and Its Role in the Biological Control of Plant Pathogens

Endophytic Fungi Isolated from Plants Growing in Central Andean Precordillera of Chile with Antifungal Activity against Botrytis cinerea

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