Screening and functional prediction of differentially expressed circRNAs in proliferative human aortic smooth muscle cells

Chen, Wei; Lin, Jiajie; Li, Bin; Cao, Shanhu; Li, Huanhuan; Zhao, Jianzhi; K, Liu; Li, Yiming; Yang, Li; Sun, Shao-Guang

doi:10.1111/jcmm.15150

Cited by 18 publications

(12 citation statements)

References 60 publications

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“…However, searching the ideal biomarker for the accurate diagnosis and effective treatment of PCOS has been a formidable challenge because its pathogenesis has not been fully elucidated. Over the past decade, circRNAs were regarded as novel and vital regulators for the expression of genes as their altered expressions had been strongly associated with a variety of diseases 29‐32 . Accumulating evidences suggest that circRNAs could be used as the excellent indicators of diagnosis and prognosis.…”

Section: Discussionmentioning

confidence: 99%

CircPSMC3 alleviates the symptoms of PCOS by sponging miR‐296‐3p and regulating PTEN expression

Ding

et al. 2020

J Cellular Molecular Medi

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Polycystic ovary syndrome (PCOS), the most common female endocrine disease that causes anovulatory infertility, still lacks promising strategy for the accurate diagnosis and effective therapeutics of PCOS attributed to its unclear aetiology. In this study, we determined the abnormal reduction in circPSMC3 expression by comparing the ovarian tissue samples of PCOS patients and normal individuals. The symptom relief caused by up‐regulation of circPSMC3 in PCOS model mice suggested the potential for further study. In vitro functional experiments confirmed that circPSMC3 can inhibit cell proliferation and promote apoptosis by blocking the cell cycle in human‐like granular tumour cell lines. Mechanism study revealed that circPSMC3 may play its role through sponging miR‐296‐3p to regulate PTEN expression. Collectively, we preliminarily characterized the role and possible insights of circPSMC3/miR‐296‐3p/PTEN axis in the proliferation and apoptosis of KGN cells. We hope that this work provides some original and valuable information for the research of circRNAs in PCOS, not only to better understand the pathogenesis but also to help provide new clues for seeking for the future therapeutic target of PCOS.

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Section: Discussionmentioning

confidence: 99%

CircPSMC3 alleviates the symptoms of PCOS by sponging miR‐296‐3p and regulating PTEN expression

Ding

et al. 2020

J Cellular Molecular Medi

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“…Using a unique source of 151 aortic SMCs from multiethnic donors, we identified 1,589 circRNAs of which 173 were differentially expressed between quiescent and proliferative SMCs. Although previous studies have performed differential expression of circRNAs between PDGF-BB treated and control vascular SMCs 11,12,23,24 , our study tested differences in circular RNA expression across SMCs cultured in the presence or absence of fetal bovine serum. While we did not treat our RNA with linear RNA degradation (Rnase R) enzyme like these other studies, we included many more samples (150 donors in two conditions).…”

Section: Discussionmentioning

confidence: 99%

Genetic Regulation of Circular RNA Expression in Human Aortic Smooth Muscle Cells and Vascular Traits

Aherrahrou

Lue

Civelek

2022

Preprint

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Background: Circular RNAs (circRNAs) are a class of non-coding RNAs that have cell-type specific expression and are relevant in cardiovascular disease. Aortic smooth muscle cells (SMCs) play a crucial role in cardiovascular disease by differentiating from a quiescent to proliferative phenotype. The role of circRNAs in SMCs and their relevance to cardiovascular disease is largely unexplored. Results: In this study, we employ a systems genetics approach to identify circRNA transcripts at a genome wide level and their relevance in cardiovascular traits. We quantified circRNA expression across 151 quiescent and proliferative human aortic SMCs from multiethnic donors. We identified 1,589 expressed circRNAs. Between quiescent and proliferative SMCs, we identified 173 circRNAs which were differentially expressed. To characterize the genetic regulation of circRNA expression, we associated the genotypes of 6.3 million single nucleotide polymorphisms (SNPs) with circRNA abundance and found 96 circRNAs which were associated with genetic loci. Three SNPs were associated with circRNA expression in proliferative SMCs but not in quiescent SMCs. We identified 6 SNPs which had distinct association directions with circRNA isoforms from the same gene. Lastly, to identify the relevance of circRNAs in cardiovascular disease, we overlapped genetic loci associated with circRNA expression with vascular disease related GWAS loci. We identified 7 blood pressure, 1 myocardial infarction, and 3 coronary artery disease loci which were associated with a circRNA transcript (circZKSCAN1, circFOXK2, circANKRD36, circLARP4, circCEP85L, circGTF3C2, circPDS5A, circSLC4A7, and chr17:42610108|42659552) but not mRNA transcript. Conclusions: Overall, our results provide mechanistic insight into the regulation of circRNA expression and the genetic basis of cardiovascular disease.

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“…Although circRNAs and lncRNAs differ significantly in shape and size, both are expressed in tissue- and spatiotemporal-specific patterns and regulate their target genes by similar mechanisms [ 33 – 36 ]. Since the ceRNA hypothesis was proposed [ 37 ], both circRNAs and lncRNAs have been reported to function as miRNA sponges that alter the stability or translation of mRNAs [ 21 , 38 ]. Thus, we used microarray and bioinformatics analyses to detect DEcircRNAs and DElncRNAs between the prefrontal cortex tissues of Nrf2 (-/-) and Nrf2 (+/+) mice.…”

Section: Discussionmentioning

confidence: 99%

Functional predication of differentially expressed circRNAs/lncRNAs in the prefrontal cortex of Nrf2-knockout mice

Gao

Zhang

Liu

et al. 2021

Aging

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In the central nervous system, nuclear factor erythroid-2-related factor 2 (Nrf2) protects neurons from oxidant injury, thereby ameliorating neurodegeneration. We explored the key circular RNAs (circRNAs) and long non-coding RNAs (lncRNAs) involved in Nrf2-induced neuroprotection. We used microarrays to examine the circRNAs (DEcircRNAs), lncRNAs (DElncRNAs) and mRNAs (DEmRNAs) differentially expressed between Nrf2 (+/+) and Nrf2 (-/-) mice and identified DEcircRNA/DElncRNA-miRNA-DEmRNA interaction networks. In total, 197 DEcircRNAs, 685 DElncRNAs and 356 DEmRNAs were identified in prefrontal cortical tissues from Nrf2 (-/-) mice. The expression patterns of selected DEcircRNAs (except for mmu_circ_0003404) and DElncRNAs in qRT-PCR analyses were generally consistent with the microarray analysis results. Functional annotation of the DEmRNAs in the DEcircRNA/DElncRNA-miRNA-DEmRNA networks indicated that five non-coding RNAs (mmu_circ_0000233, ENSMUST00000204847, NONMMUT024778, NONMMUT132160 and NONMMUT132168) may contribute to Nrf2 activity, with the help of mmu_circ_0015035 and NONMMUT127961. The results also revealed that four non-coding RNAs (cicRNA.20127, mmu_circ_0012936, ENSMUST00000194077 and NONMMUT109267) may influence glutathione metabolism. Additionally, 44 DEcircRNAs and 7 DElncRNAs were found to possess coding potential. These findings provide clues to the molecular pathways through which Nrf2 protects neurons.

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Screening and functional prediction of differentially expressed circRNAs in proliferative human aortic smooth muscle cells

Cited by 18 publications

References 60 publications

CircPSMC3 alleviates the symptoms of PCOS by sponging miR‐296‐3p and regulating PTEN expression

CircPSMC3 alleviates the symptoms of PCOS by sponging miR‐296‐3p and regulating PTEN expression

Genetic Regulation of Circular RNA Expression in Human Aortic Smooth Muscle Cells and Vascular Traits

Functional predication of differentially expressed circRNAs/lncRNAs in the prefrontal cortex of Nrf2-knockout mice

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