Screening and identification of bacteria producing polysaccharide-degrading enzymes from a Malaysian hot spring

Rahman, Zuriah Abdul; Noor, Zainon Mohd; Aziz, Aziyah Abd; Ismail, Mohamad Izwan; Azmi, Nurul Shafika; Hamzani, Nor Farah S.; Abdullah, Mohd Faiz Foong

doi:10.1109/chuser.2012.6504420

Cited by 1 publication

(1 citation statement)

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“…26 The polysaccharide itself may play a role in the identification and recognition of bacteria. 27 After the bacteria die, the LPS is completely released, triggering the immune response and inflammatory performance of the infected person, which is also one of the toxicities of the bacteria and the harm to the host. 28 Besides, CPS could prevent phagocytosis.…”

Section: Discussionmentioning

confidence: 99%

Human neutrophil peptide 1 promotes immune sterilization in vivo by reducing the virulence of multidrug‐resistant Klebsiella pneumoniae and increasing the ability of macrophages

Wang¹,

Chen

Yan

et al. 2021

Biotech and App Biochem

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By studying the expression in patients and cell modeling in vitro, antimicrobial peptides for Klebsiella were screened. Killing curve and membrane permeability experiments are used to study the antibacterial effect of antimicrobial peptides in vitro. Cytotoxicity-related indicators including lipopolysaccharide (LPS), capsule polysaccharide (CPS), and outer membrane protein expression were measured. Intranasal inoculation of pneumoconiosis was used to construct a mouse infection model, and the survival rate and cytokine expression level were tested. Human neutrophil peptide 1 (HNP-1) showed a significant antibacterial effect, which improved the permeability of the outer membrane of K. pneumoniae. Moreover, HNP-1 decreased LPS, CPS content, and outer membrane proteins. K. pneumoniae infection decreased antimicrobial peptide, oxidative stress, and autophagy-related genes, while HNP-1 increased these genes. After coculture with macrophages, the endocytosis of macrophages is enhanced and the bacterial load is greater in the K. pneumoniae + peptide group. Besides, higher levels of pp38 and pp65 in the K. pneumoniae + peptide group. HNP-1 rescued the cytotoxicity induced by K. pneumoniae. The survival rate is significantly improved after K. pneumoniae is treated by HNP-1. All cytokines in the peptide group were significantly higher. HNP-1 promotes immune sterilization by reducing the virulence of multidrug-resistant K. pneumoniae and increasing the ability of macrophages.

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Section: Discussionmentioning

confidence: 99%