Screening and Identification of Efficient Biosurfactant Producing Bacteria for some Medical Applications

Motwali, Ebtihal Abdullah; Aly, Magda M; Qari, Huda A.; Shehri, Wafa Al

doi:10.47275/0032-745x-s2-005

Cited by 2 publications

(3 citation statements)

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“…To isolate biosurfactant producing bacteria, the enrichment method was applied using the procedure that described previously by Motwali et al, (2020). Number of quantitative and qualitative methods were used to screen the biosurfactant production ability of bacterial isolates.…”

Section: Isolation and Screeningmentioning

confidence: 99%

“…Number of quantitative and qualitative methods were used to screen the biosurfactant production ability of bacterial isolates. According to technique reported by Motwali et al, (2020), Drop collapse and CTAB assay was used as qualitative method, while oil displacement test and surface tension measurement was utilized as a quantitative one. 9…”

Section: Isolation and Screeningmentioning

confidence: 99%

“…was reported by previous researchers. 9,[27][28][29] Commonly, bacterial isolates that belong to genus Pseudomonas are the greatest biosurfactant producers. 30 There are not many studies about biosurfactant produce ability by Pseudomonas mendocina and Pseudomonas oleovorans.…”

Section: Original Effect Of Nutritional Factors and Growth Conditions...mentioning

confidence: 99%

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Effect of nutritional factors and growth conditions on biosurfactant production by Pseudomonas mendocina and Pseudomonas oleovorans isolated from oil contaminated soil in Jeddah city

Motwali,

Aly,

Qari

et al. 2021

J. Contemp. Med. Sci.

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Objectives:The current study aimed to investigate the impact of different nutritional factors and growth conditions on biosurfactant production by bacterial isolates (EMB16 and EMB21) isolated from oil-contaminated soil samples. Methods: Based on the used quantitative and qualitative screening method in current study, the selected bacterial isolates showed a high potential to produce biosurfactant. Results: By using 16S rRNA sequence analysis, the bacterial isolates EMB16 and EMB21 were found to be closely related to Pseudomonas mendocina and Pseudomonas oleovorans, respectively. The ultimate yields of biosurfactant (8.06 ± 0.06 mg/ml) by Ps. mendocina EMB16 was with corn oil as a carbon source, urea as a nitrogen source, C/N ratio of 30, pH value of 7, and 2% inoculum size. For Ps. oleovorans EMB21, the maximum biosurfactant production (4.68 ± 0.14 mg/ml) was achieved by diesel oil as a carbon source, urea as a nitrogen source, C/N ratio of 30, pH of 7, and 5% size of inoculum. The best incubation period and temperature for the examined strains was 168 hrs. at 37°C. Conclusion:The results proved that Ps. mendocina EMB16 was the most efficient biosurfactant producer as it showed the greatest amount of biosurfactant concentration and lowest value of surface tension measurement with an emulsification index of 67 ± 6%.

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Section: Isolation and Screeningmentioning

confidence: 99%

Section: Isolation and Screeningmentioning

confidence: 99%

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Effect of nutritional factors and growth conditions on biosurfactant production by Pseudomonas mendocina and Pseudomonas oleovorans isolated from oil contaminated soil in Jeddah city

Motwali,

Aly,

Qari

et al. 2021

J. Contemp. Med. Sci.

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Soil Microbes Drive the Flourishing Growth of Plants From Leucocalocybe mongolica Fairy Ring

Wang

Wang²,

Wei

et al. 2022

Front. Microbiol.

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Fairy ring is a natural phenomenon in which fungal fruiting bodies occur as a ring on a spot. This ring is produced due to spore ejection by Basidiomycetous fungi and forms a lush growing plant belt. However, the drivers for such formations and the potential plant growth-promoting rhizobacteria in fairy ring soils remain unknown. Fairy rings formed by Leucocalocybe mongolica were selected in this study. Soil characteristics and microbial (bacteria and fungi) community structures between beneath and outside the fairy rings were compared through high-throughput sequencing. Beneficial bacterial resources were excavated using dependent culturable methods. Soil electrical conductivity and available potassium were higher in the soil beneath the ring than outside it. These parameters were positively correlated with the dominant microbial community, but microbial diversity was lower. In the soil beneath the fairy ring, Bacteroidetes and Basidiomycota were more abundant, whereas Verrucomicrobia was less prevalent. Bacillus pumilus (strain BG-5) was isolated from the soil beneath the ring. Strain BG-5 can solubilize phosphorus and produce indole-3-acetic acid, NH4+, and siderophores. Furthermore, strain BG-5 enhanced salt tolerance and promoted the growth of Arabidopsis thaliana, wheat (Triticum aestivum), and cotton (Gossypium hirsutum) seedlings. This study indicated the presence of abundant beneficial microbes driving the flourishing growth of plants in the fairy ring soil and provided bio-resources for agricultural growth-promoting agents.

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Screening and Identification of Efficient Biosurfactant Producing Bacteria for some Medical Applications

Cited by 2 publications

References 0 publications

Effect of nutritional factors and growth conditions on biosurfactant production by Pseudomonas mendocina and Pseudomonas oleovorans isolated from oil contaminated soil in Jeddah city

Effect of nutritional factors and growth conditions on biosurfactant production by Pseudomonas mendocina and Pseudomonas oleovorans isolated from oil contaminated soil in Jeddah city

Soil Microbes Drive the Flourishing Growth of Plants From Leucocalocybe mongolica Fairy Ring

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