Screening and identification of gut anaerobes (Bacteroidetes) from human diabetic stool samples with and without retinopathy in comparison to control subjects

Moubayed, Nadine; Bhat, Ramesa Shafi; Farraj, Dunia A. Al; Dihani, Nouf Al; Ansary, A.; Fahmy, Rania

doi:10.1016/j.micpath.2019.01.025

Cited by 30 publications

(32 citation statements)

References 25 publications

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“…Similarly, we observed that abundances of Firmicutes and related Clostridiaceae in the DM and DR groups were lower than in healthy participants. Additionally, we found that Bacteroidetes was more abundant in the DR than in the DM and HC groups, which is in line with other study findings ( Moubayed et al., 2019 ). Bacteroidetes are gram-negative bacteria whose cell walls are mainly composed of LPS.…”

Section: Discussionsupporting

confidence: 93%

“…However, it is still unclear whether differences exist in human gut microbiota between diabetic patients with and without retinopathy. Studies on gut microbial dysbiosis have identified the Bacteroidetes phylum using culture methods or have focused on mycobiome dysbiosis through Illumina sequencing of the ITS2 region ( Moubayed et al., 2019 ; Jayasudha et al., 2020 ). Therefore, this study set out to systematically detect and identify differences in gut microbial compositions of diabetic individuals with (DR) or without (DM) retinopathy and to compare each group with healthy individuals (HC) based on 16S ribosomal RNA (rRNA) gene sequencing.…”

Section: Introductionmentioning

confidence: 99%

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Dysbiosis and Implication of the Gut Microbiota in Diabetic Retinopathy

Huang

Wang

Ma³

et al. 2021

Front. Cell. Infect. Microbiol.

105

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The pathogenesis of type 2 diabetes mellitus (T2DM) is commonly associated with altered gut bacteria. However, whether the microbial dysbiosis that exists in human diabetic patients with or without retinopathy is different remains largely unknown. Here, we collected clinical information and fecal samples from 75 participants, including 25 diabetic patients without retinopathy (DM), 25 diabetic patients with retinopathy (DR), and 25 healthy controls (HC). The gut microbial composition in the three groups was analyzed using 16S ribosomal RNA (rRNA) gene sequencing. Microbial structure and composition differed in the three groups. The α and β diversities in both the DM and DR groups were reduced compared with those in the HC group. Blautia was the most abundant genus, especially in the DM group. In addition, increased levels of Bifidobacterium and Lactobacillus and decreased levels of Escherichia-Shigella, Faecalibacterium, Eubacterium_hallii_group and Clostridium genera were observed in the DM and DR groups compared with the HC group. Furthermore, a biomarker set of 25 bacterial families, which could distinguish patients in the DR group from those in the DM and HC groups was identified, with the area under the curve values ranging from 0.69 to 0.85. Of note, Pasteurellaceae, which was increased in DM and decreased in DR compared with HC, generated a high AUC (0.74) as an individual predictive biomarker. Moreover, 14 family biomarkers were associated with fasting blood glucose levels or diabetes, with most of them being negatively correlated. In summary, our study establishes compositional alterations of gut microbiota in DM and DR, suggesting the potential use of gut microbiota as a non-invasive biomarker for clinical and differential diagnosis, as well as identifying potential therapeutic targets of diabetic retinopathy.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Dysbiosis and Implication of the Gut Microbiota in Diabetic Retinopathy

Huang

Wang

Ma³

et al. 2021

Front. Cell. Infect. Microbiol.

105

View full text Add to dashboard Cite

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“…Our study detected a relationship between plasma TMAO and presence of DR, but up to date, the exact causal direction of this association remains unclear. It was indicated that diabetic gut microbiota dysbiosis could promote DR development [28], and gut microbial compositions in DWR or DR patients were different from that in controls [29]. Beli et al found that intermittent fasting could prevent the progression of DR by restructuring the gut microbiome with increased Firmicutes and decreased Bacteroidetes [30].…”

Section: Discussionmentioning

confidence: 99%

Elevated plasma trimethylamine-N-oxide levels are associated with diabetic retinopathy

Liu

Wang²,

Xia³

et al. 2020

Acta Diabetol

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Aims To determine the relationship between plasma levels of trimethylamine-N-oxide (TMAO) and odds of diabetic retinopathy (DR). Methods A cross-sectional study was conducted. Blood samples were obtained from 122 type 2 diabetes mellitus (T2DM) patients with or without DR. Multivariable logistic regression analyses were performed to identify the association between plasma TMAO and DR. The diagnostic value of plasma TMAO was assessed by the area under the receiver operating characteristic curve (AUROC) and integrated discrimination improvement (IDI). Results In the T2DM patients, plasma levels of TMAO were significantly higher in patients with DR compared with those without DR (P = 0.001). As logarithmic (ln) transformation of TMAO increased per standard deviation (SD), there was higher probability to have DR [odds ratio (OR) = 2.31; P = 0.005]. As ln-transformed TMAO increased per SD, the severity of DR was more likely to get worse (OR = 2.05; P = 0.004). In the diagnostic model, the addition of TMAO contributed to the improvement in AUROC from 0.646 to 0.734 (P = 0.043), and the IDI was 10.7% (P < 0.001). Conclusion Elevated levels of plasma TMAO were associated with higher odds and worse severity of DR in T2DM patients, and further investigation is required for the causality of this association.

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“…However, alterations of gut microbiota have not been directly linked to DR in human studies. A previous study using fecal colony culture and PCR strategy did not nd signi cant difference in the abundance of Bacteroides between diabetic patients with and without DR [9]. This is a pioneering study to explore the DR-associated alterations of human gut microbiome and metabolome.…”

Section: Discussionmentioning

confidence: 84%

Alterations of the Gut Microbiome and Metabolome in Patients with Proliferative Diabetic Retinopathy

Yao

Ye²,

Zhang³

et al. 2020

Preprint

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Background: Diabetic retinopathy (DR) has been reported to associate with gut microbiota alterations in murine models and thus “gut-retina-axis” has been proposed. However, the role of gut microbiome and the associated metabolism in DR patients still need to be elucidated. Results: Fecal samples from 45 patients with proliferative DR (PDR) and 90 matched diabetic patients (1:2 according to age, sex and duration of diabetes) without DR (NDR) were subjected to 16S rRNA gene sequencing and untargeted metabolomics. Significantly lower bacterial diversity was observed in PDR group than that in NDR group. Differential gut bacterial composition was found, with significant depletion of 22 families (e.g., Coriobacteriaceae, Veillonellaceae and Streptococcaceae) and enrichment of 2 families (Burkholderiaceae and Burkholderiales_unclassified) in PDR group as compared to NDR group. There were significantly different fecal metabolic features, which were enriched in metabolic pathways such as arachidonic acid and microbial metabolism, between the two groups. Among 36 co-abundance metabolite clusters, 11 were positively/negatively contributed to PDR using logistic regression analysis. Fifteen gut microbial families were significantly correlated with the 11 metabolite clusters. Furthermore, a fecal metabolites-based classifier was constructed to distinguish PDR patients from NDR patients accurately.Conclusions: PDR is associated with reduced diversity and altered composition of gut microbiota and specific microbe-metabolite interplay. Our findings help to better understand the disease pathogenesis and provide novel diagnostic biomarkers and therapeutic targets for PDR.

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Screening and identification of gut anaerobes (Bacteroidetes) from human diabetic stool samples with and without retinopathy in comparison to control subjects

Cited by 30 publications

References 25 publications

Dysbiosis and Implication of the Gut Microbiota in Diabetic Retinopathy

Dysbiosis and Implication of the Gut Microbiota in Diabetic Retinopathy

Elevated plasma trimethylamine-N-oxide levels are associated with diabetic retinopathy

Alterations of the Gut Microbiome and Metabolome in Patients with Proliferative Diabetic Retinopathy

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