Screening and identification of microRNAs from plasma-derived extracellular vesicles (EVs) of Dazu black goat (Capra hircus) in early pregnant stages

Zhao, Le; Yang, Haili; Rong, Zhang; Tian, Haoyu; Cheng, Lei; Cheng, Wenqiang; Zhao, Yongju

doi:10.1016/j.gene.2021.145706

Cited by 5 publications

(3 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, their resistance with the stable structure to adverse effects such as freezing–thawing and high temperature is encouraging for detecting early pregnancy and monitoring the maintenance of pregnancy ( De Bem et al, 2017 ). More specifically, in livestock animals, plasma samples from cattle and goats provide unique miRNA profiles between pregnant and non-pregnant groups, which circulating miRNAs were shown to be potential markers of early pregnancy stages and endometrial receptivity in the field of reproductive biology ( Ioannidis and Donadeu, 2016 ; Zang et al, 2021 ; Zhao et al, 2021 ). El-Shorafa and Sharif (2016) determined that healthy fertile women exhibit different expression profiles of some miRNAs in maternal plasma than women who experienced recurrent pregnancy loss of unknown cause ( El-Shorafa and Sharif, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

et al. 2022

View full text Add to dashboard Cite

MicroRNA (miRNA) plays an important role in the control of gene expression and is implied in many biological functions, including embryo implantation and development. The aim was to assess plasma miRNA profiles during the peri-implantation and ascertain potential candidate miRNA markers for early pregnancy diagnosis in ovine plasma. The plasma samples were obtained from a total of 24 ewes on days 12 (pre-implantation; P12, n = 4), 16 (implantation; P16, n = 4) and 22 (post-implantation; P22, n = 4) after mating, and on their corresponding days of 12 (Pre-C; C12, n = 4), 16 (Imp-C; C16, n = 4) and 22 (Post-C; C22, n = 4) of the estrous cycle. The miRNA profiles in plasma were assessed by microarray technology. We detected the presence of 60 ovine-specific miRNAs in plasma samples. Of these miRNAs, 22 demonstrated a differential expression pattern, especially between the estrous cycle and early pregnancy, and targeted 521 genes. Two miRNAs (oar-miR-218a and oar-miR-1185-3p) were confirmed using RT-qPCR in the ovine plasma samples. Protein-protein interaction (PPI) network of target genes established six functional modules, of which modules 1 and 3 were enriched in the common GO terms, such as inflammatory response, defense response, and regulation of immune response. In contrast, module 2 was enriched in the developmental process involved in reproduction, embryo development, embryonic morphogenesis, and regulation of the developmental process. The results indicate that miRNAs profiles of plasma seemed to be modulated during the peri-implantation stage of pregnancy in ewes. Circulating miRNAs could be promising candidates for diagnosis in early ovine pregnancy.

show abstract

Section: Introductionmentioning

confidence: 99%

Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In plasma-derived EVs of goats, 5 miRNAs were found exclusively in pregnant animals, being candidates for early pregnancy diagnosis in this species [ 99 ].…”

Section: Evs As Biomarkersmentioning

confidence: 99%

Extracellular Vesicles in Veterinary Medicine

Moccia

Sammarco

Cavicchioli

et al. 2022

Animals

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are cell-derived membrane-bound vesicles involved in many physiological and pathological processes not only in humans but also in all the organisms of the eukaryotic and prokaryotic kingdoms. EV shedding constitutes a fundamental universal mechanism of intra-kingdom and inter-kingdom intercellular communication. A tremendous increase of interest in EVs has therefore grown in the last decades, mainly in humans, but progressively also in animals, parasites, and bacteria. With the present review, we aim to summarize the current status of the EV research on domestic and wild animals, analyzing the content of scientific literature, including approximately 220 papers published between 1984 and 2021. Critical aspects evidenced through the veterinarian EV literature are discussed. Then, specific subsections describe details regarding EVs in physiology and pathophysiology, as biomarkers, and in therapy and vaccines. Further, the wide area of research related to animal milk-derived EVs is also presented in brief. The numerous studies on EVs related to parasites and parasitic diseases are excluded, deserving further specific attention. The literature shows that EVs are becoming increasingly addressed in veterinary studies and standardization in protocols and procedures is mandatory, as in human research, to maximize the knowledge and the possibility to exploit these naturally produced nanoparticles.

show abstract

“…A high lambing rate, healthy kids, and a high survival rate are key measures that improve the goat reproductive rate. Our laboratory has analyzed the regulatory mechanisms of Dazu black goat reproduction from the perspectives of the ovary [ 1 ], testis [ 2 ], embryo implantation [ 3 ], and placental development [ 4 ]. However, prior research has revealed that kid mortality rates in Chongqing during wet and cold winters [ 5 ] are also substantially higher, causing a significant impact on the profitability of the Dazu black goat industry.…”

Section: Introductionmentioning

confidence: 99%

Global gene expression profiling of perirenal brown adipose tissue whitening in goat kids reveals novel genes linked to adipose remodeling

Zhao,

Yang,

et al. 2024

J Animal Sci Biotechnol

Self Cite

View full text Add to dashboard Cite

Background Brown adipose tissue (BAT) is known to be capable of non-shivering thermogenesis under cold stimulation, which is related to the mortality of animals. In the previous study, we observed that goat BAT is mainly located around the kidney at birth, and changes to white adipose tissue (WAT) in the perirenal adipose tissue of goats within one month after birth. However, the regulatory factors underlying this change is remain unclear. In this study, we systematically studied the perirenal adipose tissue of goat kids in histological, cytological, and accompanying molecular level changes from 0 to 28 d after birth. Results Our study found a higher mortality rate in winter-born goat kids, with goat birthing data statistics. Then we used thermal imaging revealing high temperature in goat hips at postnatal 0 d and gradually decrease during 28 d. This is consistent with the region of perirenal BAT deposition and highlights its critical role in energy expenditure and body temperature regulation in goat kids. Additionally, we found a series of changes of BAT during the first 28 d after birth, such as whitening, larger lipid droplets, decreased mitochondrial numbers, and down-regulation of key thermogenesis-related genes (UCP1, DIO2, UCP2, CIDEA, PPARGC1a, C/EBPb, and C/EBPa). Then, we used RNA-seq found specific marker genes for goat adipose tissue and identified 12 new marker genes for BAT and 10 new marker genes for WAT of goats. Furthermore, 12 candidate genes were found to potentially regulate goat BAT thermogenesis. The mechanism of the change of this biological phenomenon does not involve a large-scale death of brown adipocytes and subsequent proliferation of white adipocytes. While apoptosis may play a limited role, it is largely not critical in this transition process. Conclusions We concluded that perirenal BAT plays a crucial role in thermoregulation in newborn goat kids, with notable species differences in the expression of adipose tissue marker genes, and we highlighted some potential marker genes for goat BAT and WAT. Additionally, the change from BAT to WAT does not involve a large-scale death of brown adipocytes and subsequent proliferation of white adipocytes.

show abstract

Screening and identification of microRNAs from plasma-derived extracellular vesicles (EVs) of Dazu black goat (Capra hircus) in early pregnant stages

Cited by 5 publications

References 41 publications

Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

Extracellular Vesicles in Veterinary Medicine

Global gene expression profiling of perirenal brown adipose tissue whitening in goat kids reveals novel genes linked to adipose remodeling

Contact Info

Product

Resources

About