Screening and identification of potential bioactive components in a combined prescription of Danggui Buxue decoction using cell extraction coupled with high performance liquid chromatography

Abstract

A cell extraction coupled with high-performance liquid chromatography (HPLC) analysis has been developed to screen the potential bioactive components in combined prescription of Danggui Buxue decoction. The method was validated by using HL-7702 cells, RAW 264.7 cells and Caco-2 cell extraction. According to the hypothesis that, when cells are incubated together with the extract of traditional Chinese medicines (TCMs), the potential bioactive compounds in the extract should selectively combine with the cells, cell-combining compounds were analyzed by HPLC-diode array-evaporative light scattering detectors. Their structures were elucidated in comparison with available reference compounds, and further confirmed by HPLC-electrospray ionization time-of-flight mass spectrometry with accurate mass measurement. The results demonstrated that nine compounds were combined with the HL-7702 cells, seven with RAW 264.7 cells, and thirteen with the Caco-2 cell line. In view of the two key steps of drugs action, absorption by intestinal epithelium cells and interaction with target cells, this rapid and reliable method could be utilized to predict the bioactive constituents in TCMs, and it was in agreement with the characteristics of combined prescriptions of TCMs as multi-components, multi-target sites and multi-channel actions.

Recent analytical approaches in quality control of traditional Chinese medicines—A review

“…However, the limitation of these methods should also be considered when analyses are performed. First, each model is not suitable for all of the TCMs, for example, none of the potential compound of Sophora flavescens was found in the DNA model [17]; ferulic acid, one of the main active compounds of DBD, could not be detected in several screening methods [18,22,26]. Secondly, the parallelism of different screening methods is not perfect, so it easily causes confusion, for example, the active components of DBD were different in various screening methods [12,18,22,26].…”

“…have been studied by the different cells extraction, such as endothelial cells, Caco-2 cells, macrophages, HL-7702 cells, RAW 264.7 cells, etc. [23][24][25][26].…”

“…were proposed for screening and analysis of the multiple bioactive compounds in TCMs by the groups of Zou and Li [4,9,[17][18][19][20][21][22][23][24][25][26]. HPLC or HPLC-MS is the commonly used analytical means, but according to the different sampling methods, these biofingerprint analyses are classified into three types, microdialysis, equilibrium dialysis and cell or target molecule extraction methods.…”

Establishment and drug sensitivity evaluation of murine ascites hepatocarcinoma cell line with high lymphatic metastatic potential (Hca-P/L6)

“…However, the reactivity of a variety of classes of cells to the same kind of medicine is different and the sensitivity of variant clone cell strains from the same cell line to the same kind of medicine is also different. Zhang et al screened the potential bioactive components in the combined prescription of Danggui Buxue (当归补血) decoction by a cell extraction coupled with highperformance liquid chromatography (HPLC) analysis, and the results show the number of compounds detected from HL-7702 cell, RAW 264.7 cell and Caco-2 cell extractions were 9, 7 and 13 respectively [1]. Therefore, it is necessary to select a corresponding cell strain when the effects of the medications on the specialized tissues or cells are investigated.…”

Screening and identification of potential bioactive constituents in a new herbal prescription seaweed complex preparation

“…Generally, the curative effects of TCMP result from integrative contributions of a number of bioactive constituents, and this idea is consistent with the concept of modern system biology as well as network pharmacology. TCMP also has a creative character of synergistic and competitive action among bioactive components upon multiple target sites (Zhang et al, 2008). Although many TCMPs have proven to be effective by modern pharmacological studies and clinical trials, the bioactive constituents of most TCMPs remain mysterious and unclear.…”