Screening and In Situ Monitoring of Potential Petroleum Hydrocarbon Degraders in Contaminated Surface Water

Abstract

Incomplete combustion of fossil fuels and other anthropogenic activities result in contamination of surface water by petroleum hydrocarbons. These pollutants can have severe effects on aquatic life and human health. In petroleum bioremediation, oil degrading microorganisms are utilized to remove petroleum hydrocarbons from polluted water. However, monitoring and identifying microorganisms capable of degrading petroleum hydrocarbons is very challenging. In the current study, bacteria isolated from a river alongside a petroleum refinery were assessed for their petroleum hydrocarbon degradation abilities. Primary selection was performed through traditional enrichment culture method, emulsifying ability measurement, and gravimetric analysis of hydrocarbon degradation. Screening of two novel hydrocarbon catabolism genes encoding key enzymes in hydrocarbon degradation, alkB and C23O, was performed by using polymerase chain reaction. The bacterial isolates, degrading hydrocarbons with high emulsification abilities and harboring the catabolic genes, were identified as species of Acinetobacter by 16S rRNA sequence analysis. Monitoring the temporal distribution of Acinetobacter in river water was also done by fluorescence in situ hybridization using DNA probe ACA652. The results revealed that species of Acinetobacter successfully populate the polluted surface water and have high potential for petroleum bioremediation.

Molecular biological methods in environmental engineering

“…Fluorescence in situ hybridization (FISH) technique uses an oligonucleotides probe with fluorescent tag to bind with DNA specifically. There are many successful application cases using FISH, and catalyzed reporter deposition-FISH (CARD-FISH) has established to further improve the technology in application (Chen et al, 2017a;Donde and Xiao, 2017a (Icgen and Yilmaz, 2017).…”

Succession Patterns of Microbial Composition and Activity following the Diesel Spill in an Urban River

“…Many hydrocarbon-degrading taxa across different river ecosystems have been identified in surface water with diesel contamination [ 15 , 16 ], For example, the bacterial genera of Acinetobacter and Staphylococcus populated the diesel-polluted surface water in the Kizilirmak river and Thesjaswini river, respectively, and both have remarkable capabilities to degrade diesel contaminants [ 17 , 18 ]. Fungi-containing hydrocarbon catabolic enzymes also have the potential to biodegrade diesel hydrocarbons [ 19 , 20 ].…”