Screening and isolation of antibiotic resistance inhibitors from herb materials IV-resistance inhibitors fromAnetheum graveolens andAcorus gramineus

Kim, Hye-Kyung; Moon, Kyung Ho; Ryu, Shi‐Yong; Moon, Dong-Cheul; Lee, Chung Kyu

doi:10.1007/bf02976767

Cited by 7 publications

(5 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is because of the emergence of resistant pathogens that is beyond doubt the consequence of years of widespread indiscriminate use, incessant and misuse of antibiotics (14,39). One of the methods to reduce the resistance to antibiotics is by using antibiotic resistance inhibitors from plants (3,21).It is expected that plant extracts showing target sites other than those used by antibiotics will be active against drug resistant pathogens (1).Medicinal plants have been used as traditional treatments for numerous human diseases for thousands of years and in many parts of the world. Hence, researchers have recently paid attention to safer phytomedicines and biologically active compounds isolated from plant species used in herbal medicines with acceptable therapeutic index for the development of novel drugs (29,38).Socotra is distinguished by strong biodiversity and endemism.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical screening and in vitro antibacterial activity of Cissus subaphylla and Euphorbia spiralis endemic in Socotra Island

Al-Asbahi,

Alasbahi

2016

Univ. Aden J. Nat. and Appl. Sc.

View full text Add to dashboard Cite

Cissus subaphylla and Euphorbia spiralis, endemic in Socotra Island, were screened for their chemical constituents and antibacterial activity. The phytochemical screening of the chloroform and the 70% ethanol extracts of both plant stems indicated the presence of sterols, triterpenoids, cardiac glycosides, anthraquinons, flavonoids, tannins and carbohydrates as chemical constituents. Testing the antibacterial activity of chloroform and the 70% ethanol extracts (5 and 10mg) of both plants against Staphylococcus aureus (305-864-669), Staphylococcus epidermidis (505-864-689), Pseudomonas aeruginosa(909-825-1793) and Escherichia coli(909-825-1793), using agar well diffusion assay, demonstrated that all tested extracts displayed a significant antibacterial activity with activity index (AI) above 0.5 against test microorganisms, except the chloroform extract of C. subaphylla, which was found inactive against S. aureus. The 70% ethanol extract (10mg)of E. spiralis showed the highest antibacterial activity (AI=0.80-0.94) against E. coli.

show abstract

Section: Discussionmentioning

confidence: 99%

Phytochemical screening and in vitro antibacterial activity of Cissus subaphylla and Euphorbia spiralis endemic in Socotra Island

Al-Asbahi,

Alasbahi

2016

Univ. Aden J. Nat. and Appl. Sc.

View full text Add to dashboard Cite

show abstract

“…Combinations of antibiotic are common in an allopathic therapeutic system and even available in the commercial market too but the synergistic effect of the plant extract and allopathic drug against different microbes are still in practice very less commonly (Gurib-Fakim, 2006). Several types of research showing the activity of hexane and methanol extract of the rhizome of Acorus species significantly inhibited various drug-resistant strains of Staphylococcus aureus while tested with Ampicillin, Chloramphenicol and benzyl benzoate in vitro (Kim et al, 1998). For a certain strain of Extended-Spectrum Beta-Lactamase producing E. coli, in vitro synergism was observed between the respective crude extracts of A. calamus with either tetracycline or ciprofloxacin (Ahmad and Aqil, 2007).…”

Section: Research Articlementioning

confidence: 99%

Antimicrobial and Cytotoxic activity of rhizome extract of Acorus calamus (Bojho) in combination with different antimicrobial agents: Synergistic Effects

Khanal¹

2019

Int J Appl Sci Biotechnol

View full text Add to dashboard Cite

Acorus calamus (sweet flag) is a monocot plant found in wetland, have the scented leaves and rhizomes. Various active bio-ingredients of A. calamus had been studied and characterized and some of them are known for antimicrobial and antitumor activities. The dry rhizomes were powdered ethanolic extraction was performed in a Soxhlet apparatus. The extract was dried and re-suspended to sterile distilled water and sterilized by membrane filtration. The synergist affects against bacteria, fungi, helminths were evaluated and cytotoxic assay was performed. The minimum inhibitory concentrations of plant extract was found to be 50µg/ml for the vancomycin resistance Staphylococcus aureus, for extended spectrum beta lactamase Escherichia coli, Methicillin resistant S. aureus, were 100 µg/ml. Synergistically cefixime and cefpodoxime both antibiotics are found to be effective against all strains of S. aureus and E. coli except VRSA. The antifungal characteristics were found to be effective when agar cup diffusion were performed in combination with fluconazole antifungal drug. The combination of plant extract was more effective anthelminthic drug than the anthelminthic drug alone. Similarly, the plant extract has lethal concentration 50 (LC50) was found to be 173.3 µg / ml and LC90 was 555. 4 µg / ml on brine shrimp. The A. calamus has potential characteristics to be as antimicrobial and antitumor medicine when used synergistically with antimicrobial agents.

show abstract

“…This is because of the emergence of resistant pathogens that is beyond doubt the consequence of years of widespread indiscriminate use, incessant and misuse of antibiotics [13][14] Antibiotic resistance has increased substantially in the recent years and is posing an ever-increasing therapeutic problem. One of the methods to reduce the resistance to antibiotics is by using antibiotic resistance inhibitors from plants [15][16]. Plants are known to produce a variety of compounds to protect themselves against a variety of pathogens.…”

Section: Introductionmentioning

confidence: 99%

PHYTOCHEMICAL, ANTIMICROBIAL, RADICAL SCAVENGING AND In-Vitro BIOLOGICAL ACTIVITIES OF Teucrium stocksianum LEAVES

Rahim

Qureshi

Ali

et al. 2023

J. Chil. Chem. Soc.

View full text Add to dashboard Cite

Background: Teucrium stocksianum is locally used as home remedy in different parts of the world for the treatment of digestive problems as well as microbial infections. The present study was designed to screen phytochemical constituents and in vitro antimicrobial as well as radical scavenging activities of the leaves extracts from the Teucrium stocksianum. Methods: Plant samples of the Teucrium stocksianum were collected from the growing locality and four different solvents viz., nhexane, methanol, ethanol, and water were used for the preparation of plant extracts. The in vitro biological activities were investigated against eight human pathogens such as like Bacteria: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogen, Escherichia coli, Klebsiella pneumoniae and Pseusdomonas aeruginosa and Fungi: Aspergillus niger and Aspergillus fumigatus using agar well diffusion method and minimum inhibitory concentration. The antioxidant properties were carried out by DPPH and reducing power assays using various concentrations. Results: Results revealed that all pathogens were highly susceptible and inhibited by all solvent extracts. The methanolic and ethanolic leaves extracts exhibited promising antimicrobial activity and inhibited the growth of pathogens at par with the standard drugs viz., chloramphenicol for Bacteria and Fluconazole for fungi. Moreover, performance of methanolic and ethanolic extracts was the best amongst all solvents. In terms of antioxidant properties, both methods i.e., DPPH free radicals and reducing power resulted significant activity. Conclusion: The Phytochemical screening and biological activities of Teucrium stocksianum leaves extracts is reported for the first time. The ethanol, methanol, n-hexane and aqueous extracts of Teucrium stocksianum possess significant inhibitory effect against tested pathogens. The present investigation Teucrium stocksianum leaves extract contain potential antimicrobial components that may be of great use for the development of pharmaceutical industries as a therapy against various diseases. These results denote in-depth analysis of methanolic and ethanolic extracts to search potential compound responsible for antimicrobial activity. The results of the study support the folklore claim along with the development of new antimicrobial drugs from the plant. The results showed that Teucrium stocksianum leaves extracts possess multiple essential phytochemicals. Presence of phenolic compounds in plant extract demonstrates antioxidant activity. Both tests for antioxidant properties exhibited marked effects and can be employed as a potential natural antioxidant agent which may be used for mitigating oxidative stress. Nevertheless, there is need to explore mechanism involved for such kind of activity. The current findings hold up the ethno-pharmacological utilization of plant in the treatment of microbial infections and embrace great perception in the development of unusual antimicrobial and antioxidant agents.

show abstract

Screening and isolation of antibiotic resistance inhibitors from herb materials IV-resistance inhibitors fromAnetheum graveolens andAcorus gramineus

Cited by 7 publications

References 10 publications

Phytochemical screening and in vitro antibacterial activity of Cissus subaphylla and Euphorbia spiralis endemic in Socotra Island

Phytochemical screening and in vitro antibacterial activity of Cissus subaphylla and Euphorbia spiralis endemic in Socotra Island

Antimicrobial and Cytotoxic activity of rhizome extract of Acorus calamus (Bojho) in combination with different antimicrobial agents: Synergistic Effects

PHYTOCHEMICAL, ANTIMICROBIAL, RADICAL SCAVENGING AND In-Vitro BIOLOGICAL ACTIVITIES OF Teucrium stocksianum LEAVES

Contact Info

Product

Resources

About