Screening and metabolic potential of fungal strains isolated from contaminated soil and sediment in the polychlorinated biphenyl degradation

Germain, Joaquim; Raveton, Muriel; Binet, Marie‐Noëlle; Mouhamadou, Bello

doi:10.1016/j.ecoenv.2020.111703

Cited by 30 publications

(20 citation statements)

References 42 publications

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“…Instead, the degrading activity of soil‐dwelling fungi is poorly studied. Some species of filamentous fungi belonging to the genus Penicillium, Aspergillus, Scedosporium, Doratomyces, Myceliophthora, Phoma and Thermoascus were isolated from PCBs polluted soils, and their degradation potential was characterized in a few studies (Tigini et al ., 2009 ; Mouhamadou et al ., 2013 ; Germain et al ., 2021 ). PCB depletion is thought to occur mainly through the activity of laccases, however the biodegradation pathways of these microorganisms need further investigation.…”

Section: Pcbs ‐Degradative Potential Of Soil Microbiomesmentioning

confidence: 99%

‘Cry‐for‐help’ in contaminated soil: a dialogue among plants and soil microbiome to survive in hostile conditions

Rolli

Vergani

Ghitti

et al. 2021

Environmental Microbiology

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Summary An open question in environmental ecology regards the mechanisms triggered by root chemistry to drive the assembly and functionality of a beneficial microbiome to rapidly adapt to stress conditions. This phenomenon, originally described in plant defence against pathogens and predators, is encompassed in the ‘cry‐for‐help’ hypothesis. Evidence suggests that this mechanism may be part of the adaptation strategy to ensure the holobiont fitness in polluted environments. Polychlorinated biphenyls (PCBs) were considered as model pollutants due to their toxicity, recalcitrance and poor phyto‐extraction potential, which lead to a plethora of phytotoxic effects and rise environmental safety concerns. Plants have inefficient detoxification processes to catabolize PCBs, even leading to by‐products with a higher toxicity. We propose that the ‘cry‐for‐help’ mechanism could drive the exudation‐mediated recruitment and sustainment of the microbial services for PCBs removal, exerted by an array of anaerobic and aerobic microbial degrading populations working in a complex metabolic network. Through this synergistic interaction, the holobiont copes with the soil contamination, releasing the plant from the pollutant stress by the ecological services provided by the boosted metabolism of PCBs microbial degraders. Improving knowledge of root chemistry under PCBs stress is, therefore, advocated to design rhizoremediation strategies based on plant microbiome engineering.

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Section: Pcbs ‐Degradative Potential Of Soil Microbiomesmentioning

confidence: 99%

‘Cry‐for‐help’ in contaminated soil: a dialogue among plants and soil microbiome to survive in hostile conditions

Rolli

Vergani

Ghitti

et al. 2021

Environmental Microbiology

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“…First, this result suggests that these species were stable and tolerant to environmental stress. Interestingly, among these species, Stachybotrys chartarum, F. oxysporum, Penicillium canescens, P. chrysogenum and P. citrosulfuratum had previously been isolated from soil D, while P. chrysogenum, P. brevicompactum and F. oxysporum had already been isolated from other soils highly polluted with PCBs [19,20,22], suggesting their dominance and ubiquity in PCB-polluted soils. The fact that the abundance of species was variable according to the soils could confirm the regulation of PCBs and/or physico-chemical properties on these species, consistent with the studies showing the effect of PCBs on the fungal biomass [28].…”

Section: Discussionmentioning

confidence: 99%

“…Second, among the 16 species, the strains of F. solani, P. canescens, P. citrosulfuratum and P. chrysogenum had already shown interesting abilities for the PCB degradation in liquid medium by displaying biodegradation rates of at least 60% after 7 days of incubation [20,22]. More interestingly, some strains of these species used in consortium in the mycoremediation by bioaugmentation have made it possible to obtain remarkable biodegradation rates using soils highly polluted with PCBs [21,23].…”

Section: Discussionmentioning

confidence: 99%

“…Mycoremediation strategies primarily use model fungal species of ligninolytic Basidiomycota due to their interesting biochemical potential, particularly their capacity to produce the nonspecific oxidative exoenzymes involved in the degradation of numerous organic pollutants [7,[16][17][18]. Mycoremediation strategies are also based on the use of native fungal species isolated directly from PCB-contaminated soils and generally used in consortium, i.e., a mixture of several strains previously screened for their biodegradation efficiency [19][20][21][22][23]. While the ligninolytic Basidiomycota displayed partial or low PCB degradation rates compared to those expected probably due to their non-telluric origin, their non-adaptation to PCB-polluted soils or their difficulty to grow in soils due to the competition with indigenous microbiota [13,24], native fungal species could be promising [21,23].…”

Section: Introductionmentioning

confidence: 99%

“…The GenBank accession number of the sequences of fungal species used as reference species are indicated in brackets. The species represented in red and blue are those previously isolated from soil D[22] and from another PCB-polluted soil[20], respectively. The 16 species present in the 3 studied soils are in bold.…”

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Molecular Characterization of Fungal Biodiversity in Long-Term Polychlorinated Biphenyl-Contaminated Soils

et al. 2021

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Polychlorinated biphenyls (PCBs) belong to the organic pollutants that are toxic to humans and harmful to environments. Numerous studies dealing with the impact of PCBs on soil microorganisms have focused on bacterial communities. The effects of PCBs on fungal communities in three different PCB-polluted soils from former industrial sites were investigated using high-throughput sequencing of the internal transcribed spacer 1 region. Significant differences in fungal alpha diversity were observed mainly due to soil physico-chemical properties. PCBs only influenced the richness of the fungal communities by increasing it. Fungal composition was rather strongly influenced by both PCBs and soil properties, resulting in different communities associated with each soil. Sixteen Ascomycota species were present in all three soils, including Stachybotrys chartarum, Fusarium oxysporum, Penicillium canescens, Penicillium chrysogenum,Penicillium citrosulfuratum and Penicillium brevicompactum, which are usually found in PCB-polluted soils, and Fusarium solani, Penicillium canescens, Penicillium citrosulfuratum and Penicillium chrysogenum, which are known PCB degraders. This study demonstrated that PCBs influence the richness and the composition of fungal communities. Their influence, associated with that of soil physico-chemical properties, led to distinct fungal communities, but with sixteen species common to the three soils which could be considered as ubiquitous species in PCB-polluted soils.

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Insight into the protein degradation during the broad bean fermentation process

Lin

Zhou

Zhao

et al. 2022

Food Science & Nutrition

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Broad bean fermentation is of vital importance in PixianDouban (PXDB) production, as well as a key process for microorganisms to degrade protein, which lays the foundation for the formation of PXDB flavor. In this study, two fungi and bacteria were screened, and their morphology, molecular biology, growth, and enzyme production characteristics were analyzed, and then they were applied to the broad bean fermentation simulation system. The protein, peptide, amino acid, amino nitrogen, and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) in the system were evaluated. The results showed that the four microorganisms were Aspergillus oryzae , Aspergillus jensenii , Staphylococcus gallinarum , and Enterobacter hormaeche . Aspergillus oryzae had the highest protease activity at pH 7.0, while the other three strains had better enzyme activity stability under neutral acidic conditions. And the total protein (F1 and F2 were 18.32 g/100 g, 19.15 g/100 g, respectively), peptides (11.79 ± 0.04 mg/g and 12.06 ± 0.04 mg/g), and amino acids (55.12 ± 2.78 mg/g and 54.11 ± 1.97 mg/g) of the fungus experimental groups (F) were higher than the bacterial experimental groups (B). In addition, the enzyme system produced by fungi exhibited a stronger ability for albumin (20 kDa) and glutenin (<30 kDa) deterioration in neutral conditions, while the bacterial enzyme system was more efficient in degrading albumin (<30 kDa) and glutenin (20–30 kDa) in acidic conditions, as indicated by SDS‐PAGE. These findings showed that both bacteria and fungi played an important role in the degradation of protein in different fermentation stages of broad bean fermentation. Practical applications There is a lack of comprehensive understanding of the protein composition and protein degradation mechanism of broad beans in the fermentation stage of PXDB. This research work explored the differences in the degradation of PXDB fermented protein by different microorganisms, and provided a theoretical basis for optimizing the production of PXDB and improving the quality of PXDB.

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Screening and metabolic potential of fungal strains isolated from contaminated soil and sediment in the polychlorinated biphenyl degradation

Cited by 30 publications

References 42 publications

‘Cry‐for‐help’ in contaminated soil: a dialogue among plants and soil microbiome to survive in hostile conditions

‘Cry‐for‐help’ in contaminated soil: a dialogue among plants and soil microbiome to survive in hostile conditions

Molecular Characterization of Fungal Biodiversity in Long-Term Polychlorinated Biphenyl-Contaminated Soils

Insight into the protein degradation during the broad bean fermentation process

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