Screening and Molecular Identification of New Microbial Strains for Production of Enzymes of Biotechnological Interest

Ghazala, Imen; Haddar, Anissa; Romdhane, Molka Ben; Ellouz-Chaanouni, Semia

doi:10.1590/1678-4324-2016150152

Cited by 26 publications

(13 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ghazala et al obtained two clear zones of citrus pectin (1%) hydrolysis and a single clear zone of xylan (0.2%) hydrolysis after staining with ruthenium red and Congo red, respectively, for analyzing pectinase and xylanase activity regions. Karimi observed two clear zones of pectinase enzyme activity on a polyacrylamide gel containing 1% commercial pectin after treatment with ruthenium red.…”

Section: Resultsmentioning

confidence: 99%

Development of novel economical methodologies for zymographic analysis of purified xylano–pectinolytic enzymes using agrowaste‐based substrates

Agrawal

Kaur

Varghese

et al. 2019

Biotechnology Progress

View full text Add to dashboard Cite

In this study, zymographic analysis for xylanase and pectinase enzymes has been carried out using agrowaste residues, wheat bran and citrus peel as well as their extracts. Isozymic forms of xylanase as well as pectinase enzyme displayed comparable zymographic bands onto agar petriplates containing either commercial substrates (xylan and pectin), agrowaste‐based substrates (wheat bran and citrus peel), or polysaccharides extracted from these agrowastes (crude xylan and pectin extracted from wheat bran and citrus peel, respectively), indicating the fact that agro residues and their extracts can be utilized as a substitute of cost‐intensive commercial substrates, xylan and pectin for zymographic analysis. This is the first report revealing the zymographic analysis of xylano–pectinolytic enzymes using agro‐based solid residues particles or polysaccharides extracted from agro‐based residues.

show abstract

Section: Resultsmentioning

confidence: 99%

Development of novel economical methodologies for zymographic analysis of purified xylano–pectinolytic enzymes using agrowaste‐based substrates

Agrawal

Kaur

Varghese

et al. 2019

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…Very limited literature is available so far about B. mojavensis and this is the first report presenting the production of chitosanase by B. mojavensis. Previously, B. mojavensis was reported to produce few valuable industrial enzyme such as xylanase [15], protease [7,16], α-amylase, [16] and pectinase [17] but, chitosanase production ability of B. mojavensis was not reported earlier.…”

Section: Discussionmentioning

confidence: 99%

Optimization of chitosanase production by Bacillus mojavensis EGE‐B‐5.2i

et al. 2018

View full text Add to dashboard Cite

Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest. The present study was conducted to improve chitosanase activity of an indigenous strain identified as Bacillus mojavensis. Initially, carbon and nitrogen sources were optimized by one-variable-at-a-time approach. Further, fermentation medium was optimized using Plackett-Burman (PB) and central composite designs (CCD). PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production. CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L , respectively to achieve maximum chitosanase activity (21.1 U ml ). Discovery of the novel optimal medium has improved chitosanase production by B. mojavensis up-to 9.5 folds. Lastly, 18.6 U ml chitosanase activity was achieved in stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase.

show abstract

“…Retting and degumming of flax straw is an important step in textile industry. The retting, is a process employing the action of micro-organisms and moisture on plants to dissolve or rot away much of the cellular tissues and pectins surrounding bast-fiber bundles, and so facilitating separation of the fiber from the stem [13,14]. In the wet retting process of the flax, bundles of flax stems are to be immersed in water for fermentation by anaerobic bacteria to degrade pectins and matrix components in the plant cell wall [17].…”

Section: Introductionmentioning

confidence: 99%

Retting and degumming of flax using biotechnology eco-friendly approach

Moawad¹,

El‐Rahim

Hashem

et al. 2019

Egypt. J. Chem.

View full text Add to dashboard Cite

F LAX retting is a treatment process employed to dissolve or rot away much of the cellular tissues and pectins surrounding bast/fiber bundles thus facilitating separation of the fiber from the stem. The microbial retting is one of the most environmentally friendly process. The rich source of microbes contributing to flax retting is the retting water in commercial retting plants. The main objective of this work is to accelerate the flax retting using selected bacterial strains contributing to retting and degumming of flax in order to avoid chemicals causing environmental pollution. In this work, reuse of retting water for acceleration of flax retting was tested. The results show that, the reuse of retted water in new cycle of flax retting has accelerated the retting completion by 8.3-25% depending on the ratio of reused retted water in the retting liquor. Therefore, samples of flax straw retted water and soils from flax fields located in Nile Valley and Delta soils were collected for isolation of flax retting bacteria predominant in the retting effluents. Identification of the isolates was done using morphological, physiological and biochemical tests. Ten bacterial isolates were identified as Lactococcus cremoris, Chryseobacterium culicis, Serratia marcescens, Capnocytophage granulose, Micrococcus luteus, Enterobacter homraechei, Klebsiella oxytoca, Paenibacillus polymyxa, Proteus mirabilis and Bacillus humi. Three efficient strains in pectinase production necessary for flax retting were selected to test their role in enhancing the retting process. These strains are Bacillus humi, Chryseobacterium culicis and Micrococcus luteus which showed specific pectinase activity 37.17, 33.53 and 28 Umg 1 respectively. The effect of these strains on retting duration and fiber quality were studied. The combinations of the three strains were tested in the lab scale cylindrical experimental bioreactor and each of the combinations was compared with the classical retting process of the industrial units. The combined treatment using strains: Chryseobacterium culicis, Micrococcus luteus and Bacillus humi resulted in the reduction of the retting duration by 30%, whereas, the mixed inocula containing Chryseobacterium culicis and Micrococcusluteus reduced the retting time by 25%. The mixture of Micrococcus luteus and Bacillus humi reduced the retting duration by 20%. The determination of weight loss, tensile strength, whiteness and yellowness of the fibers after microbial retting was monitored. These results indicate that the obtained specific bacterial strains enhanced the retting process.

show abstract

Screening and Molecular Identification of New Microbial Strains for Production of Enzymes of Biotechnological Interest

Abstract: This research focused on isolation, identification and characterization of new strains of fungi and bacteria

Cited by 26 publications

References 46 publications

Development of novel economical methodologies for zymographic analysis of purified xylano–pectinolytic enzymes using agrowaste‐based substrates

Development of novel economical methodologies for zymographic analysis of purified xylano–pectinolytic enzymes using agrowaste‐based substrates

Optimization of chitosanase production by Bacillus mojavensis EGE‐B‐5.2i

Retting and degumming of flax using biotechnology eco-friendly approach

Contact Info

Product

Resources

About