“Screening and Optimization of Agro-industrial wastes for glycoprotein biosurfactant production from Sphingobacterium thalpophilum DP9”

Abstract

Background Commercialization of biosurfactant production is a big challenge due to high production cost. Biosurfactant production can be made economic by using low cost agro-industrial wastes or byproducts as media supplement. It also solves the problem of environmental pollution through waste management. In the present study attempt was made to produce biosurfactant from Sphingobacterium thalpophilum DP9 using various agro-industrial wastes or byproducts at optimum fermentation conditions evaluated by traditional and statistical methods. Partial characterization of biosurfactant was also carried out through qualitative chromatographic techniques; quantitative spectroscopic method and functional group identification by Fourier transform infrared spectroscopy. Results One factor at a time optimization experimentation showed highest emulsification in media supplemented with potato peel powder (43.12 ± 3.41%), urea (46.00 ± 3.09%), pH 7 (41.74 ± 1.15%) at 40% aeration (47.41 ± 1.62%). 1% Inoculum (O.D.600=1.00) size favored highest emulsification (26.39 ± 1.60%). Plackett-Burman design experimentation showed carbon source (potato peel powder), nitrogen source (urea) and temperature significantly affect on biosurfactant production. Response surface experimentation by central composite design showed interaction between nitrogen source and temperature significantly influenced on biosurfactant production. Validation of model was showed increase in emulsification from 43.62 ± 2.55% to 86.11 ± 3.47% on fourth day of incubation. Partial characterization showed biosurfactant contains 439.58 ± 0.0129 µg/ml carbohydrate and 507.41 ± 0.0064 µg/ml of protein. Lipid was absent in it. Fourier transform infrared spectroscopy chromatogram showed peaks at 1652.17 cm− 1, 1541.01 cm− 1, 1463.71 cm− 1 and 722.32 cm− 1 which indicated presence of peptide bond, peptide moiety, carbohydrate protons and primary as well as secondary amines. Conclusion Potato peel powder, urea and temperature have significant influence on biosurfactant production from Sphingobacterium thalpophilum DP9. When potato peel powder and urea supplied at optimum level, biosurfactant production was increased by two fold. Biosurfactant produced by S. thalpophilum DP9 was belongs to glycoprotein class and as per our best knowledge, this is the first report on glycoprotein biosurfactant produced from Sphingobacterium genus.

Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276

“…The presence of aliphatic chains (–CH2 and –CH3 groups) is suggested by the peaks that appeared at around 2925 cm −1 , which can be attributed to the –CH stretching vibrations [ 35 ]. The peak at 3284 cm –1 suggests the presence of stretching vibrations from the –NH of the peptide portion [ 39 ]. Furthermore, the spectrum points to the presence of stretching vibrations in the transmittance region of 1635 cm −1 (amide I bond) and 1547 cm −1 (amide II bond), thus confirming the presence of proteins [ 40 ].…”

Efficacy of biosurfactant as a coating material for post-harvest preservation of jamun fruit (Syzygium cumini)

“…They can be produced from agro-industrial waste or byproducts, which also solve the problem of waste disposal (Solanki et al 2020). This group of compounds includes glycolipids, phospholipids, glycoproteins, lipoproteins and lipopolysachharides.…”

Production of an ultrasound-assisted biosurfactant postbiotic from agro-industrial wastes and its activity against Newcastle virus

“…Despite the several benefits of assembling microbial biosurfactants, the commercialization is hampered due to the fact that fermentation processes have a low yield, which makes them expensive to produce, not being able to economically compete with synthetic surfactants ( 3 ). Expensive raw materials constitute 10–30% of the total cost of large-scale bioprocesses, are the main concern for biosurfactant production ( 10 , 11 ). One strategy into making biosurfactants production more cost-effective is the replacement of synthetic substrates by agro-food wastes and renewable substrates representing an additional advantage as well as a decrease in environmental concern while it provides a high-product yield ( 11 – 13 ).…”

“…One strategy into making biosurfactants production more cost-effective is the replacement of synthetic substrates by agro-food wastes and renewable substrates representing an additional advantage as well as a decrease in environmental concern while it provides a high-product yield ( 11 – 13 ). The correct balance of nutrients is an important factor to select the right biowastes for microbial growth and production of metabolites like biosurfactants ( 10 , 14 ). Currently, a wide range of renewable raw substrates like various industrial and agricultural by-products and wastes are available that can be employed as nitrogen and carbon sources as well as other nutrient minerals for industrial fermentation processes ( 10 , 12 ).…”

“…The correct balance of nutrients is an important factor to select the right biowastes for microbial growth and production of metabolites like biosurfactants ( 10 , 14 ). Currently, a wide range of renewable raw substrates like various industrial and agricultural by-products and wastes are available that can be employed as nitrogen and carbon sources as well as other nutrient minerals for industrial fermentation processes ( 10 , 12 ). Research studies have demonstrated the use of alternative substrates for the production of biosurfactants.…”