Screening and Probiotic Potential Evaluation of Bacteriocin-Producing Lactiplantibacillus plantarum In Vitro

Abstract

Probiotics are gaining attention due to their functions of regulating the intestinal barrier and promoting human health. The production of bacteriocins is one of the important factors for probiotics to exert beneficial properties. This study aimed to screen bacteriocin-producing Lactiplantibacillus plantarum and evaluate the probiotic potential in vitro. It was found that L. plantarum Q7, L. plantarum F3-2 and L. plantarum YRL45 could produce bacteriocins and inhibit common intestinal pathogens. These three strains had probiotic potential with tolerance to the gastrointestinal environmental and colonization in the gut, and exhibited various degrees of anti-inflammatory activity and tight junction function in the intestinal barrier. Particularly, L. plantarum YRL45 could significantly (p < 0.05) reduce the increase in nitric oxide (NO), prostaglandin E2 (PGE2), necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS), thereby easing inflammatory response. L. plantarum F3-2 could remarkably (p < 0.05) up-regulate the expression levels of ZO-1, Occludin and Claudin-1 in intestinal epithelial injured cells, which was conducive to protecting the intestinal barrier. These findings provided fundamental information about the probiotic properties of bacteriocin-producing L. plantarum, which suggested that L. plantarum Q7, L. plantarum F3-2 and L. plantarum YRL45 had the potential to be used as novel probiotic strains.

Molecular identification and safety assessment of the potential probiotic strain Bacillus paralicheniformis HMPM220325 isolated from artisanal fruit dairy products

“…The concentration of bile salts in the gastrointestinal tract of humans typically varies between 0.3% and 0.5%. 65 B. paralicheniformis HMPM220325 grows normally in bile salts ranging from 0% to 0.9%, suggesting that this strain exhibits remarkable tolerance to bile salts and can effectively utilize them for growth, aligning with the predictions made based on its genomic characteristics. It is worth mentioning that the BSH activity of probiotics has long been a topic of discussion because of its dual effect.…”

Probiotics: mechanism of action, health benefits and their application in food industries

“…Probiotics also act as anti-microbial agents by producing substances; short chain fatty acids (SCFA), organic acids, hydrogen peroxide ( Ahire et al, 2021 ), and bacteriocins ( Fantinato et al, 2019 ) thus decreasing pathogenic bacteria in the gut. Moreover, probiotics improve the intestinal barrier function by stimulating the production of mucin proteins ( Chang et al, 2021 ), regulating the expression of tight junction proteins, including occluding and claudin 1, and regulating the immune response in the gut ( Bu et al, 2022 ; Ma et al, 2022 ).…”

Genome Sequence and Evaluation of Safety and Probiotic Potential of Lactiplantibacillus plantarum LPJZ-658

“…The present study revealed that L. plantarum LPJZ-658 had higher cell hydrophobicity and auto-aggregation compared to LGG, indicating its good cell adhesion properties. S. thyphimurium , E. coli , and S. aureus are common intestinal pathogens that are highly susceptible to causing infections in the body, promoting an inflammatory response in the intestinal tract, destroying the intestinal barrier, and causing diarrhea and other diseases [ 72 ]. Previous studies had reported the antimicrobial activity of probiotics [ 73 ], which in agreement with our study, the L. plantarum LPJZ-658 showed excellent antimicrobial activity against pathogenic bacteria, including S. thyphimurium , E. coli , and S. aureus .…”