Screening and selection of media components for lactic acid production using Plackett–Burman design

Kappalli, Sudha; Prapulla, S. G.; Rajalakshmi, D.; Misra, Mahesh Chandra; Karanth, N. G.

doi:10.1007/s004490050483

Cited by 12 publications

(20 citation statements)

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“…Therefore, it is very difficult to determine the C/N ratio of these components. One-factor-at-a-time for determination of C/N ratio and media composition, a typical method used for optimizing a multivariable system, not only is time consuming, but is also difficult to find the interaction of factors [7][8][9][10]. However, the Plackett-Burman experimental design (PBD) is very useful in identifying the important nutrients for cell growth.…”

Section: Statistical Medium Optimizationmentioning

confidence: 99%

“…High cell density cultivation through medium optimization was studied by various statistical experimental designs [7,10,13]. One-factor-at-a-time, a typical method used for optimizing a multivariable system not only is time consuming, but also is difficult to determine the interaction of factors [7][8][9][10]. However, a statistical experimental design, named the Plackett-Burman experimental design (PBD), is very useful in identifying the significant nutrients for cell growth or enzyme production.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of culture media for Bacillus species by statistical experimental design methods

Cho

Kim

2009

Korean J. Chem. Eng.

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The medium compositions of Bacillus species (three strains) were optimized sequentially through two statistical experimental designs, Plackett-Burman and the Box-Behnken, which were determined by representative threefactor response-surface methodology. pH and DO control was found to enhance the viable cell number of Bacilli. Most strains grew well in the optimum condition and reached nearly up to of 5×10 9 CFU/mL. Additionally, when three of the Bacilli were cultivated in the optimized medium with pH and DO control, the viable cell number increased 3.6 times in B. subtilis, 64.1 times in B. licheniformis and 8.3 times in B. coagulans, respectively, in comparison with the original industrial medium.

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Section: Statistical Medium Optimizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of culture media for Bacillus species by statistical experimental design methods

Cho

Kim

2009

Korean J. Chem. Eng.

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“…This design allows reliable short listing of a small number of ingredients for further optimization and allows one to obtain unbiased estimates of linear effects of all the factors with maximum accuracy for a given number of observations, the accuracy being the same for all effects (Krishnan et al 1998). The present study aims to investigate the production of GOD using A. niger NCIM 545 using the one-factor-at-a-time classical method, Placket-Burman design, and response surface methodology (RSM).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Aspergillus niger Fermentation for the Production of Glucose Oxidase

Bankar

Bule

Singhal

et al. 2008

Food Bioprocess Technol

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A number of nutritional factors influencing glucose oxidase (EC 1.1.3.4) production by Aspergillus niger NCIM 545 were studied. The synthesis of glucose oxidase by A. niger was investigated in two steps using submerged fermentation at 30±2°C and 180 rpm for 96 h. Primarily, nutritional components were selected by onefactor-at-a-time method, and the significance of each component with respect to glucose oxidase production was identified by Plackett-Burman design (seven variables including six nutritional viz. sucrose, sodium nitrate, peptone, calcium carbonate, magnesium sulfate, and potassium dihydrogen phosphate, and one dummy or unassigned variable were studied with eight experiments). In the second step, concentration of most significant factors and their interaction were studied with response surface methodology (central composite design). Each variable in the design was studied at five different levels, with all variables taken at a central coded value of zero. Considerable amount of glucose oxidase was produced from A. niger species with sucrose as the carbon source, sodium nitrate as the inorganic nitrogen source, and peptone as the organic nitrogen source. Glucose oxidase activity increased remarkably by 28.93 fold (from 0.00993 to 0.29 U ml −1 ) with CaCO 3 -supplemented media. The outcome of PlackettBurman design showed CaCO 3 , peptone, and MgSO 4 as significant parameters. Further optimization using a threefactor central composite design with 20 experiments increased yield of glucose oxidase from 0.29 to 2.05 U ml −1 (sevenfold) with a decrease in cultivation time from 96 to 72 h.

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“…This is a very economical design with the number of experiments are being greatly reduced while providing two levels (high and low) of screening design (Krishnan et al 1998). In our context, PBD is extremely useful in validating the effects of vital factors in hydrolysing the pretreated S. binderi for subsequent extraction of fermentable sugar.…”

Section: Discussionmentioning

confidence: 99%

“…This is an economical design with the number of experiments are being greatly reduced while providing two levels (high and low) screening design (Krishnan et al 1998). Table 1 outlines the important variables under investigation, and each variable was represented in two levels, high (+) and low (−).…”

Section: Methodsmentioning

confidence: 99%

Statistical optimization of fermentable sugar extraction from the Malaysian brown alga Sargassum binderi

et al. 2014

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Statistical optimisation techniques were applied in the present study to extract fermentable sugar (FS) from indigenous brown seaweed, Sargassum binderi. As identified by the Plackett-Burman design (PBD) with five variables, the most significant parameters (p<0.05) affecting sugar yield were pretreatment temperature, concentration of sulphuric acid (H 2 SO 4 ) used during pretreatment, and loading of enzyme cellulase during hydrolysis process. Upon this, the extraction of FS from S. binderi was further optimized with central composite design (CCD) by using response surface methodology (RSM). Analysis of variance revealed that every independent variable in the present study possesses significant positive effect towards the yield of FS. In addition, interactions among the independent variables were observed as well. The model proposed in this study fits significantly well to the experimental data with more than 95 % confidence. The optimal condition as proposed by the cubic model for maximum yield of FS was found as follows: pretreatment of S. binderi with 6.38 % of dilute H 2 SO 4 at 120.7°C, followed by hydrolysis using 0.16 mL of cellulase loading per unit gram of pretreated dry seaweed. The results of validation experiments gave FS yield of 1.69 g g −1 which fitted well with predicted value by RSM (1.72 g g −1 ). The overall error was small indicating the proficiency of the models in optimizing extraction of FS from S. binderi. The present study indicated that the brown seaweed S. binderi has potential to become a dependable biomass source for production of value added products.

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Screening and selection of media components for lactic acid production using Plackett–Burman design

Cited by 12 publications

References 0 publications

Optimization of culture media for Bacillus species by statistical experimental design methods

Optimization of culture media for Bacillus species by statistical experimental design methods

Optimization of Aspergillus niger Fermentation for the Production of Glucose Oxidase

Statistical optimization of fermentable sugar extraction from the Malaysian brown alga Sargassum binderi

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