Screening and verification of microRNA promoter methylation sites in hepatocellular carcinoma

Ni, Xiaofeng; Lin, Zhuo; Dai, Shengjie; Chen, Hao; Chen, Jianhui; Zheng, Chenlei; Wu, Boda; Ao, Jian‐Yang; Shi, Ke‐Qing; Sun, Hongwei

doi:10.1002/jcb.29656

Cited by 5 publications

(3 citation statements)

References 42 publications

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“…Using The Cancer Genome Atlas (TCGA) database, we downloaded BSG expression data related to TC and para-cancerous tissues [23]. Then, the downloaded data were integrated and transferred into the quantile normalized log2 format to improve measurement accuracy.…”

Section: Bsg Mrna Expression In Tc Samples From Tcga Rna-seq Datamentioning

confidence: 99%

Immunohistochemical basigin expression level in thyroid cancer tissues

et al. 2020

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Background: Thyroid cancer (TC) is the most common endocrine malignancy; basigin (also known as BSG) plays a crucial role in tumor cell invasion, metastasis, and angiogenesis. This study was designed to identify the change of BSG expression in TC and its possible potential mechanism. Methods: The BSG expression levels in TC were demonstrated using data collected from in-house immunohistochemical (IHC), RNA-sequencing (RNA-seq), microarrays, and literatures. Integrated analysis was performed to determined BSG expression levels in TC comprehensively. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed with the integration of BSG co-expressed genes and differentially expressed genes (DEGs) in TC tissues to explore the potential mechanisms of BSG in TC. Results: The protein expression level of BSG was significantly higher in TC cases based on the IHC experiments. In addition, the combined SMD for BSG expression was 0.39 (p < 0.0001), the diagnostic odds ratio was 3.69, and the AUC of the sROC curve was 0.6986 using 1182 TC cases and 437 non-cancerous cases from 17 independent datasets. Furthermore, BSG co-expressed genes tended to be enriched in gene terms of the extracellular matrix (ECM), cell adhesion, and cell-cell interactions. The expression levels of nine hub BSG co-expressed genes were markedly upregulated in TC cases. Conclusion: BSG expression levels were closely correlated with the progression of TC and may affect the signals of the ECM, cell adhesion, and cell-cell interactions.

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Section: Bsg Mrna Expression In Tc Samples From Tcga Rna-seq Datamentioning

confidence: 99%

Immunohistochemical basigin expression level in thyroid cancer tissues

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Using The Cancer Genome Atlas (TCGA) database, we downloaded BSG expression data related to TC and para-cancerous tissues [22]. Then, the downloaded data were integrated and transferred into the quantile normalized log2 format to improve measurement accuracy.…”

Section: Bsg Mrna Expression In Tc Samples From Tcga Rna-seq Datamentioning

confidence: 99%

Immunohistochemical Basigin Expression Level in Thyroid Cancer Tissues

Guo

Tang

Pang

et al. 2020

Preprint

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Background Thyroid cancer (TC) is the most common endocrine malignancy, Basigin (also known as BSG) plays a crucial role in tumor cell invasion, metastasis, and angiogenesis. This study was designed to identify the change of BSG expression in TC and its possible potential mechanism. Methods The BSG expression levels in TC were demonstrated using data collected from in-house immunohistochemical (IHC), RNA-sequencing (RNA-seq), microarrays and literatures. Integrated analysis was performed to determined BSG expression levels in TC comprehensively. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed with the integration of BSG co-expressed genes and differentially expressed genes (DEGs) in TC tissues to explore the potential mechanisms of BSG in TC. Results The protein expression level of BSG was significantly higher in TC cases based on the IHC experiments. In addition, the combined SMD for BSG expression was 0.39 (p < 0.0001), the diagnostic odds ratio was 3.69, and the AUC of the sROC curve was 0.6986 using 1182 TC cases and 437 non-cancerous cases from 17 independent datasets. Furthermore, BSG co-expressed genes tended to be enriched in gene terms of the extracellular matrix (ECM), cell adhesion, and cell-cell interactions. The expression levels of nine hub BSG co-expressed genes were markedly up-regulated in TC cases. Conclusion BSG expression levels were closely correlated with the progression of TC and may affect the signals of the ECM, cell adhesion, and cell-cell interactions.

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“…MicroRNAs (miRNAs), short 20-22 nucleotides, were recognized to hold essential parts in tumor progression by binding to the 3′untranslated region (3′-UTR) of the target mRNAs [28][29][30][31]. Analysis of miRNA expression has identified a group of dysregulated miRNAs in HCC [32]. According to the bioinformatics results (TargetScan and miRDB) and our previous study showed that ZEB1 may be a target of miR-708.…”

Section: Introductionmentioning

confidence: 99%

ZEB1 serves an oncogenic role in the tumourigenesis of HCC by promoting cell proliferation, migration, and inhibiting apoptosis via Wnt/β-catenin signaling pathway

Yang

Fan

et al. 2021

Acta Pharmacol Sin

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Zinc finger E-box-binding homeobox 1 (ZEB1), a functional protein of zinc finger family, was aberrant expressed in many kinds of liver disease including hepatic fibrosis and Hepatitis C virus. Bioinformatics results showed that ZEB1 was abnormally expressed in HCC tissues. However, to date, the potential regulatory role and molecular mechanisms of ZEB1 are still unclear in the occurrence and development of HCC. This study demonstrated that the expression level of ZEB1 was significantly elevated both in liver tissues of HCC patients and cell lines (HepG2 and SMMC-7721 cells). Moreover, ZEB1 could promote the proliferation, migration, and invasion of HCC cells. On the downstream regulation mechanism, ZEB1 could activate the Wnt/β-catenin signaling pathway by upregulating the protein expression levels of β-catenin, c-Myc, and cyclin D1. Novel studies showed that miR-708 particularly targeted ZEB1 3′-UTR regions and inhibited the HCC cell proliferation, migration, and invasion. Furthermore, results of nude mice experiments of HCC model indicated that miR-708 could inhibit tumor growth and xenograft metastasis model was established to validate that miR-708 could inhibit HCC cell metastasis through tail-vein injection in vivo. Together, the study suggested that ZEB1 modulated by miR-708 might be a potential therapeutic target for HCC therapy.

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Screening and verification of microRNA promoter methylation sites in hepatocellular carcinoma

Cited by 5 publications

References 42 publications

Immunohistochemical basigin expression level in thyroid cancer tissues

Immunohistochemical basigin expression level in thyroid cancer tissues

Immunohistochemical Basigin Expression Level in Thyroid Cancer Tissues

ZEB1 serves an oncogenic role in the tumourigenesis of HCC by promoting cell proliferation, migration, and inhibiting apoptosis via Wnt/β-catenin signaling pathway

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