Screening antibacterial activity of entomopathogenic bacteria isolated from pests of hazelnut

Uğraş, Serpil; Demirbağ, Zihni

doi:10.2478/s11756-013-0210-6

Cited by 2 publications

(9 citation statements)

References 26 publications

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“…In a previous study, high antibacterial activity of Sm-Mm3 was determined against several indicator microorganisms by well diffusion assay (Ugras et al, 2013). Results of this study demonstrated that the antibacterial substance was active against several bacteria, including Bacillus thuringiensis strains, B. cereus, B. polymyxa, B. subtilis, Enterobacter sp., Enterococcus faecalis, Erwinia amylovora, Proteus mirabilis, Paucimonas lemoignei, Pseudomonas fluorescens, P. syringae, Staphylococcus cohni, S. epidermidis, and Xanthomonas axonopodis.…”

Section: Resultsmentioning

confidence: 81%

“…In a previous study, high antibacterial activity of Sm-Mm3 was determined against several indicator microorganisms by well diffusion assay (Ugras et al, 2013 (Ugras et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…Among these bacteria, Bacillus thuringiensis subsp. thompsoni HD542 was chosen as the indicator strain to use in the next steps because of its high sensitivity to this substance (Ugras et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…It is also known that S. marcescens strains produce a significant secondary metabolite as antibiotics and/or bacteriocins that have antibacterial activity against pathogenic bacteria (Ugras et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Production of an antibacterial substance by Sm-Mm3 was detected in a previous study (Ugras et al, 2013). In this study, the modified well diffusion method was used to detect the activity spectrum of the antibacterial substance produced by the Sm-Mm3 strain as described earlier (Padilla et al, 1996;Chanda et al, 2013;Khan et al, 2013).…”

Section: Detection Of Inhibitory Spectrummentioning

confidence: 94%

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Purification and characterization of an antibacterial substance produced by pest-originated Serratia marcescens Mm3

Uğraş¹,

Sezen²,

Katı³

et al. 2014

Turk J Biol

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IntroductionSince the exploration of penicillin as a natural antibiotic and its subsequent production on a massive scale, antibiotics have revolutionized approaches to human health (Bennett and Chung, 2001). They have been used in human healthcare and in different fields such as the agricultural industry and veterinary medicine. As a result of the widespread use of antibacterials, antibiotic-resistant pathogens have been widely and continuously reported by the World Health Organization (WHO, 2000). In addition, synthetic chemicals that can negatively affect the environment and human and animal health have been used as antimicrobials (Hodgson and Levi, 1996;Zwir-Ferenc and Biziuk, 2004). Therefore, researchers always aim to find safe alternatives, such as natural and environmentally friendly antimicrobials (Tronsmo and Hjeljord, 1998).The need for novel antimicrobials to combat increasing antibiotic resistance in pathogenic bacteria has stimulated the exploration of nontraditional sources, such as terrestrial actinomycetes or fungi (Berdy, 2005). Marine organisms have been a rich source of novel antibacterial compounds (Zhang et al., 2005). Unfortunately, knowledge about antimicrobial substances of microorganisms isolated from pests is very limited.Serratia is a genus of gram-negative, facultative anaerobic, and rod-shaped bacteria of the family Enterobacteriaceae. The most common species in the genus, S. marcescens, is normally the only pathogen, and usually causes nosocomial infections (Hejazi and Falkiner, 1997). The ability of S. marcescens to inhabit a wide variety of ecological niches has been linked to its extracellular products, including proteases, chitinases, nucleases, lipases, wetting agents, bacteriocins, and the bright red pigment 2-methyl-3-penthyl-6-methoxyprodigiosin or prodigiosin (Matsuyama et al., 1995;Thomson et al., 1997). In addition, the genus Serratia is able to produce β-lactam antibiotic and 1-carbapen-2-em-3-carboxylic acid (carbapenem; Car) (Williams and Quadri, 1980;Parker et al., 1982). Furthermore, secondary metabolites such as antibiotics and bacteriocins produced by the genus Serratia are very important in various industrial fields because of their antibacterial activities. Additionally, several Serratia strains have the potential to control insect pests (Sezen and Demirbag, 2006;Jeong et al., 2010) and plant-pathogenic bacteria (Ordentlich et al., 1998).In the current study, in order to determine a novel antibacterial substance as an alternative to existing antimicrobials, we characterized an antibacterial substance

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Section: Resultsmentioning

confidence: 81%

“…In a previous study, high antibacterial activity of Sm-Mm3 was determined against several indicator microorganisms by well diffusion assay (Ugras et al, 2013 (Ugras et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Detection Of Inhibitory Spectrummentioning

confidence: 94%

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Purification and characterization of an antibacterial substance produced by pest-originated Serratia marcescens Mm3

Uğraş¹,

Sezen²,

Katı³

et al. 2014

Turk J Biol

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Screening and Molecular Identification of Bacteria from the Midgut of Amphimallon solstitiale Larvae Exhibiting Antagonistic Activity against Bacterial Symbionts of Entomopathogenic Nematodes

Skowronek

Sajnaga

Kazimierczak

et al. 2021

IJMS

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Entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) are a group of organisms capable of infecting larvae of insects living in soil, including representatives of the family Scarabaeidae. Their insecticidal activity is related to the presence of symbiotic bacteria Xenorhabdus spp. or Photorhabdus spp. in the alimentary tract, which are released into the insect body, leading to its death caused by bacterial toxins and septicemia. Although the antibacterial activities of symbionts of entomopathogenic nematodes have been well described, there is insufficient knowledge of the interactions between these bacteria and microorganisms that naturally inhabit the alimentary tract of insects infested by nematodes. In this study, 900 bacterial strains isolated from midgut samples of Amphimallon solstitiale larvae were tested for their antagonistic activity against the selected five Xenorhabdus and Photorhabdus species. Cross-streak tests showed significant antibacterial activity of 20 isolates. These bacteria were identified as Bacillus [Brevibacterium] frigoritolerans, Bacillus toyonensis, Bacillus wiedmannii, Chryseobacterium lathyri, Chryseobacterium sp., Citrobacter murliniae, Enterococcus malodoratus, Paenibacillus sp., Serratia marcescens and Serratia sp. Since some representatives of the intestinal microbiota of A. solstitiale are able to inhibit the growth of Xenorhabdus and Photorhrhabdus bacteria in vitro, it can be assumed that this type of bacterial interaction may occur at certain stages of insect infection by Steinernema or Heterorhabditis nematodes.

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Screening antibacterial activity of entomopathogenic bacteria isolated from pests of hazelnut

Cited by 2 publications

References 26 publications

Purification and characterization of an antibacterial substance produced by pest-originated Serratia marcescens Mm3

Purification and characterization of an antibacterial substance produced by pest-originated Serratia marcescens Mm3

Screening and Molecular Identification of Bacteria from the Midgut of Amphimallon solstitiale Larvae Exhibiting Antagonistic Activity against Bacterial Symbionts of Entomopathogenic Nematodes

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