Screening Antibacterial Activity of Vietnamese Plant Extracts Against Human Pathogenic Bacteria

Ngo, Long Hoang; Nguyen, Thi Hai Yen; Tran, Vu Khac; Đoán, Vũ Văn; Nguyen, Minh Van; Bui, Trung Hieu

doi:10.22159/ajpcr.2021.v14i6.39790

Cited by 3 publications

(1 citation statement)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, there has been an increased mortality rate due to complications of infectious diseases and treatment failure. This explains why resistance to antibiotics has been recognized by the World Health Organization as the greatest threat in the treatment of infectious diseases [3, 4]. For example, methicillin-resistant Staphylococcus aureus (MRSA) has been found to be the major cause of hospital-acquired infections and community-acquired infections [5].…”

Section: Introductionmentioning

confidence: 99%

Metabolomic profiling and bactericidal effect of Polyalthia longifolia (Sonn.) Twaites. stem bark against methicillin-resistant Staphylococcus aureus

Siwe

Simo

Maharjan

et al. 2023

Access Microbiology

View full text Add to dashboard Cite

Objective. The present study was carried out to establish the chemical profile of the methanolic extract of Polyalthia longifolia stem bark and investigate its antibacterial property against some human pathogenic bacteria. Methods. The extract was analysed using liquid and gas chromatography coupled to mass spectrometry. Antibacterial activity of P. longifolia extract against some human pathogenic bacteria was screened using the AlamarBlue method, and MIC and MBC were determined. Results and Conclusion. Liquid chromatography-mass spectrometry (LC-MS) revealed the presence of 21 compounds among which 12 were identified. Gas chromatography-mass spectrometry (GC-MS) allowed identification of 26 compounds, the three major ones being the following: cis vaccenic acid (17.79 %), 3-ethyl-3-hydroxyandrostan-17-one (13.80 %) and copaiferic acid B (12.82 %). P. longifolia extract was active against Gram-positive bacteria with MIC ranging from 1 to 2 mg ml−1 and MBC from 2 to 6 mg ml−1. This study demonstrated the bactericidal effect of the methanolic extract of Polyalthia longifolia stem bark against some human pathogenic bacteria, including methicillin-resistant S. aureus . This effect could be related to the presence in the extract of a broad diversity of well-known compounds with established pharmacological properties. These results support the ethnomedicinal use of P. longifolia stem bark in Cameroon for the management of methicillin-resistant S. aureus (MRSA)-related infections.

show abstract

Section: Introductionmentioning

confidence: 99%

Metabolomic profiling and bactericidal effect of Polyalthia longifolia (Sonn.) Twaites. stem bark against methicillin-resistant Staphylococcus aureus

Siwe

Simo

Maharjan

et al. 2023

Access Microbiology

View full text Add to dashboard Cite

show abstract

Characterization of Alternaria porri causing onion purple blotch and its antifungal compound magnolol identified from Caryodaphnopsis baviensis

Kim

Han²,

Dang³

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

Alternaria porri (Ellis) Clf. causes purple blotch disease on Allium plants which results in the reduction of crop yields and quality. In this study, to efficiently find natural antifungal compounds against A. porri, we optimized the culture condition for the spore production of A. porri and the disease development condition for an in vivo antifungal assay. From tested plant materials, the methanol extracts derived from ten plant species belonging to the families Cupressaceae, Fabaceae, Dipterocarpaceae, Apocynaceae, Lauraceae, and Melastomataceae were selected as potent antifungal agents against A. porri. In particular, the methanol extract of Caryodaphnopsis baviensis (Lec.) A.-Shaw completely inhibited the growth of A. porri at a concentration of 111 μg/ml. Based on chromatographic and spectroscopic analyses, a neolignan compound magnolol was identified as the antifungal compound of the C. baviensis methanol extract. Magnolol showed a significant inhibitory activity against the spore germination and mycelial growth of A. porri with IC50 values of 4.5 and 5.4 μg/ml, respectively. Furthermore, when magnolol was sprayed onto onion plants at a concentration of 500 μg/ml, it showed more than an 80% disease control efficacy for the purple blotch diseases. In terms of the antifungal mechanism of magnolol, we explored the in vitro inhibitory activity on individual oxidative phosphorylation complexes I–V, and the results showed that magnolol acts as multiple inhibitors of complexes I–V. Taken together, our results provide new insight into the potential of magnolol as an active ingredient with antifungal inhibitory action to control purple blotch on onions.

show abstract

Phytochemical Screening and in Vitro Antimicrobial and Antifungal Activity of Bergenia Ligulata (Wall.) Leaves Extracts

KUMAR¹,

Wal²,

KUMAR³

et al. 2022

Int J Pharm Pharm Sci

View full text Add to dashboard Cite

Objective: Evaluation of Antimicrobial and Antifungal potential of Phytoconstituents present in leaf extracts of Bergenia ligulata (wall.) plant. Methods: The antimicrobial effects of leaves of Bergenia ligulata (wall.) were evaluated using both ethanol and aqueous extracts against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Penicillium spp. Agar well diffusion method was employed in determining the antimicrobial activity and the broth dilution method for determining the Minimum Inhibitory Concentration. All the isolates examined were susceptible to both the ethanol, aqueous, and the combined aqueous and ethanol extracts (synergetic effect) of the Bergenia ligulata leaves. Results: The zone of inhibition ranged from 6.93±0.02b mm to 12.81±0.03a mm, with Escherichia coli being the most susceptible at 12.81±0.03a mm to the ethanol and 11.53±0.02a mm to the aqueous extracts at 250 mg/ml concentration while P. aeruginosa and Penicillin spp. were the slightest susceptible at 10.88±0.02a mm to the ethanol and 09.76±0.02a mm to the aqueous extract at 250 mg/ml concentration. The control/standard antimicrobial agent (Gentamicin and Metronidazole) exhibited higher inhibitory activity than the plant extracts. The least inhibitory value of 6.25 mg/ml was produced against P. aeruginosa by the ethanolic extract and against C. albicans by the combined (aqueous and ethanol) extracts of the plant. The qualitative and quantitative phytochemical screening of the leaves of Bergenia ligulata reveals the presence of flavonoids, tannins, saponins, alkaloids, and steroids. The most abundant percentage composition observed was flavonoids (7.72%), while tannins had the least component (4.29%). Conclusion: The findings from this study show that the leaves extracts hold considerable antimicrobial activity against commonly encountered microorganisms in the environment. This, therefore, implies that it can be used as a chemotherapeutic agent which will contribute to the development of antibiotic drugs against the test organisms.

show abstract

Screening Antibacterial Activity of Vietnamese Plant Extracts Against Human Pathogenic Bacteria

Cited by 3 publications

References 17 publications

Metabolomic profiling and bactericidal effect of Polyalthia longifolia (Sonn.) Twaites. stem bark against methicillin-resistant Staphylococcus aureus

Metabolomic profiling and bactericidal effect of Polyalthia longifolia (Sonn.) Twaites. stem bark against methicillin-resistant Staphylococcus aureus

Characterization of Alternaria porri causing onion purple blotch and its antifungal compound magnolol identified from Caryodaphnopsis baviensis

Phytochemical Screening and in Vitro Antimicrobial and Antifungal Activity of Bergenia Ligulata (Wall.) Leaves Extracts

Contact Info

Product

Resources

About