Screening bioactive compounds with multi-targets from Rhodiola crenulata by a single column containing co-immobilized beta2-adrenergic receptor and voltage dependent anion channel isoform 1

Authenticity and quality evaluation of different Rhodiola species and commercial products based on NMR‐spectroscopy and HPLC

“…Therefore in China products that contain Rhodiola species different from R. crenulata are hence considered as adulterated 19 . For that reason there are an increasing number of reports focused on the phytochemical profiles of different Rhodiola species 24–28 . The most important compounds from a pharmaceutical standpoint in R. rosea are the phenylethanoids salidroside and p ‐tyrosol, and the phenylpropanoids rosin, rosavin and rosarin (collectively named rosavins) located in the rhizomes and roots of the plant 27 .…”

“…Thus, starting as a holistic approach to identify the possible active molecules of a herbal mixture, these approaches have been utilised in uncovering unique fingerprints and biomarker molecules that are responsible for the pharmacological activity and quality assessment of plant derived preparations 37 . In our research group, NMR‐based metabolomics platform has been exclusively applied to clarify the chemotaxonomic classification of different Verbascum species, 38 study the specific metabolites or their variations in different plant parts of several medicinal species, such as Clinopodium vulgare , 37 Sambucus ebulus , 39 R. rosea 6,26 or its commercial preparations 7 …”

Development and evaluation of poly adenosine 5′‐diphosphate‐ribose polymerase 1 immobilization‐based receptor chromatography

“…immobilized β 2 ‐adrenoceptor ( β 2 ‐AR), an important drug target for respiratory diseases, onto macroporous silica gel by various immobilization methods [15–18]. The affinity columns were used to screen the bioactive compounds from TCMs, such as Siraitia grosvenorii [19] and Rhodiola crenulate [20]. They identified rosamaric acid, mogroside V, and salidroside as the bioactive compounds in the three herbal medicines that target β 2 ‐AR, respectively.…”