Screening diploidSolarium genotypes responsive to different Anther culture conditions and ploidy assessment of Anther-derived roots and plantlets

Aziz, Ahmad Naseer; Seabrook, Janet E. A.; Tai, G. C. C.; Jong, H. De

doi:10.1007/bf02853552

Cited by 12 publications

(2 citation statements)

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“…In S. chacoense we evaluated 3 genotypes and none of them showed regeneration. Cappadocia et al (1984) found differences in response among different genotypes and genotype-dependent culture response differences are well documented (Guha-Mukherjee 1973, Wenzel et al 1977, Chaleff and Stolarz 1981, Sonnino et al 1989, Aziz et al 1999, Paz and Veilleux 1999. There is also evidence that the ability to undergo embryogenesis from microspores in potato is a heritable trait, controlled by the recessive alleles of more than one gene (Sonnino et al 1989).…”

Section: Pretreatments and Genotype Screeningmentioning

confidence: 99%

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Gametic Embryogenic Response in Wild Diploid Solanum Species and its Implications for Genome Sequencing Projects and Breeding

Castillo

Gaiero

Carro

et al. 2016

Plant Tissue Cult. & Biotech.

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The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L-cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding.

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Section: Pretreatments and Genotype Screeningmentioning

confidence: 99%

“…Both cultivated and wild potato genotypes differ in their potential for embryogenesis and regeneration in anther culture (Sonnino et al 1989, Aziz et al 1999, Paz and Veilleux 1999. Genotype is the main endogenous factor affecting regeneration (Germanà 2011).…”

Section: Introductionmentioning

confidence: 99%