Screening donor blood for malaria by polymerase chain reaction

Hang, Vu Thi Ty; Be, Tran Van; Tran, Phan Ngoc; Thanh, Le Thiet; Hien, Luong Van; O'Brien, Estella; Morris, Glenn E.

doi:10.1016/0035-9203(95)90652-5

Cited by 45 publications

(12 citation statements)

References 8 publications

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“…24,25 When the results of the QBC analysis are compared with a PCR gold standard, the sensitivity was only 9.78%. This is much lower than the relative sensitivity of microscopic detection versus the PCR in previously published studies, 8,25,30 and may be due to the low level of average parasitemia in our study population, and also might reflect sensitivity problems associated with delayed reading of our samples.…”

Section: Discussioncontrasting

confidence: 41%

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Epidemiologic tools for malaria surveillance in an urban setting of low endemicity along the Colombian Pacific coast.

Carrasquilla

Banguero²,

Sánchez³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

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Abstract. An evaluation of 3 different methods for malaria diagnosis was carried out in an urban area of low endemicity on the Pacific coast of Colombia. Samples were collected from 833 symptomatic patients at a malaria clinic and examined by the polymerase chain reaction (PCR), quantitative buffy coat (QBC; Becton Dickinson, Franklin Lakes, NJ) method, and the traditional thick blood smear. The prevalence of Plasmodium falciparum malaria was 5.88% by thick blood smear, 7.34% by the QBC method, and 21.87% by PCR. The agreement between microscopists was 99.5%. The agreement between the QBC method and thick blood smear was 96.13% (n ϭ 745). Samples positive by PCR but negative by thick blood smear or conversely negative by PCR and positive by thick blood smear were usually of low-level parasitemias. All 3 methods showed agreement in 76.3% of the samples. Sixty-nine (18.8%) samples were positive by PCR but negative by the other 2 methods. Ten samples were positive by both the QBC method and thick blood smear but negative by PCR; most of them had low-level parasitemias. The use of malaria diagnostic methods for epidemiologic surveillance is discussed.

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Section: Discussioncontrasting

confidence: 41%

“…8,30 Our study population is in an area of low endemicity, where parasitemias and prevalence are typically low. Studies in other settings with higher endemicity may show greater sensitivity by microscopic techniques due to higher average levels of parasitemia.…”

Section: Discussionmentioning

confidence: 99%

Epidemiologic tools for malaria surveillance in an urban setting of low endemicity along the Colombian Pacific coast.

Carrasquilla

Banguero²,

Sánchez³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

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“…Conclusion: Although there have been many previous publication on the detection of malaria and identification of plasmodium species by PCR, all relates either to the use of some form of nested PCR, using nested or heminested primers (Snounou et al 6 ., 1993a,b; Rubio et al 37 ., 1999; Gal et al 45 , 2001), or to the detection of amplified DNA using labeled, specific probes or ELISA (Laoboonchai et al 46 , 2001), for species identification. Single round PCR has only been used alone to detect a particular species, usually P. falciparum (Tham et al 23 ., 1999; Barker et al 10 ., 1994; Hang et al 40 ., 1995), or simply to detect malarial infection without species identification (Ciceron et al 8 , 1999). The method is relatively quick, compared to nested and hemi-nested PCR, and can be completed within 3 h of sample receipt (Patsoula E et al 33 ,2003).…”

Section: Abstracts Of Some Studies On Comparing Pcr With Microscopy Amentioning

confidence: 99%

“…In a study conducted by Jamshaid Iqbal et al 39 ., in 2002 in Department of Microbiology, Faculty of Medicine, Kuwait University, and Malaria Laboratory, Department of Community Health, Ministry of Health, Safat, Kuwait titled "Comparison of the OptiMAL test with PCR for the detection of Plasmodium falciparum and Plasmodium vivax infection diagnosis of malaria in immigrants from endemic tropical countries" using microscopy and a PCR as reference standards in 550 immigrants who presented with fever The PCR and species identification were performed as described by Hang et al 40 . with slight modifications.…”

Section: Abstracts Of Some Studies On Comparing Pcr With Microscopy Amentioning

confidence: 99%

Molecular Methods for Diagnosis of Malaria

Shukla¹,

Pant²

2013

jemds

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“…For such a large scale study, usage of the RAPD technique would be difficult to carry out. To resolve this problem, we propose the use of the PCR technique that is able to detect specific sequences of the P. falciparum genome in whole-blood sample extracts contaminated by human DNA (Tirasophon et al 1991;Hang et al 1995). Furthermore, PCR is more reproducible than RAPD (Ellsworth et al 1993).…”

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confidence: 99%

Detection of RAPD Markers Correlated with Chloroquine Resistance in Plasmodium falciparum

Lescuyer

Picot²,

Bracchi³

et al. 1997

Genome Res.

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We described the use of the random amplified polymorphic DNA (RAPD) technique on Plasmodium falciparum DNA to detect genetic markers for chloroquine-resistant strains. Fourteen RAPD primers were tested, three of which generated banding patterns correlated with chloroquine resistance. To measure this correlation, the RAPD profiles were analyzed using the Nei and Li similarity coefficient. Detection of distinctive RAPD bands allowed us to synthesize specific PCR primers to be used on whole-blood samples. Two primer sets were synthesized and tested on sensitive and resistant strains for their ability to amplify the DNA fragment corresponding to the RAPD marker. These results suggest that RAPD and PCR techniques can be used as powerful tools for the detection of genetic markers associated with drug resistance.[The nucleotide sequence data described in this paper have been submitted to the EMBL, GenBank, and DDBJ Nucleotide Sequence databases under accession nos. A863W25C and U854T571.] Malaria, with an estimated 300 to 500 million cases and 1.5-2.7 million deaths per year (World Health Organization 1996), is a major health problem. Today, one of the main obstacles encountered in the control of this disease is the rapid spread of drug resistant Plasmodium falciparum strains (Slater 1993). Chloroquine, the drug most widely used in the world, is the main issue involved with this problem. Extensive literature deals with biochemical and genetic mechanisms of chloroquine resistance but there is still no substantial data available to assess the precise mechanisms underlying this phenomenon. Chloroquine-resistant parasites are known to accumulate less of the drug in their digestive vacuole than sensitive parasites, however the biochemical basis is yet to be determined as to whether it is linked with increased efflux of pre-accumulated drug or reduced chloroquine uptake (Slater 1993). The hypothesis of a rapid efflux system linked to the Pfmdr 1 gene (Krogstad et al. 1987;Wilson et al. 1989;Foote et al. 1990) has now been questioned by a number of authors (Slater 1993;Cremer et al. 1995). The genetic basis is also controversial. Data has been published suggesting the linkage of chloroquine resistance with either a unique locus (Wellems et al. 1991) or a multigenic mechanism (Karcz and Cowman 1991;Ward et al. 1995).In this context, it would be of great interest to determine genomic profiles of malaria parasites and to compare those obtained with chloroquinesensitive and chloroquine-resistant P. falciparum strains. As no similar data is available, we performed the random amplified polymorphic DNA (RAPD) analysis, which represents a powerful tool for genome characterization (Welsh and McClellend 1990;Williams et al. 1990;Lymbery 1996), on 13 different strains (5 sensitive and 8 resistant to chloroquine). The Nei and Li (NL) coefficient (Nei and Li 1979) was used to score the DNA polymorphisms. The purpose of this study was to find a correlation between RAPD banding patterns and the behavior of the parasite in relation to c...

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Screening donor blood for malaria by polymerase chain reaction

Cited by 45 publications

References 8 publications

Epidemiologic tools for malaria surveillance in an urban setting of low endemicity along the Colombian Pacific coast.

Epidemiologic tools for malaria surveillance in an urban setting of low endemicity along the Colombian Pacific coast.

Molecular Methods for Diagnosis of Malaria

Detection of RAPD Markers Correlated with Chloroquine Resistance in Plasmodium falciparum

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