Screening for bacterial vaginosis: a novel application of artificial nose technology.

Chandiok, S; Crawley, B.; Oppenheim, Beryl; Chadwick, P R; Higgins, Stephen P.; Persaud, Krishna

doi:10.1136/jcp.50.9.790

Cited by 44 publications

(26 citation statements)

References 4 publications

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“…128 (2) The screening for bacterial vaginosis in vaginal swabs seems to be feasible. 129 Newer publications even give the impression that the reliability is comparable to that of present tests and show the possibility of controlling treatment of bacterial vaginosis by tracking the acetic acid concentration with a conducting polymer array. 130,131 (3) Common bacterial pathogens of the upper respiratory tract were obtained from in vitro samples and successfully detected by a Cyranose 320 electronic nose.…”

Section: Disease Diagnosismentioning

confidence: 76%

Electronic Nose: Current Status and Future Trends

2008

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Section: Disease Diagnosismentioning

confidence: 76%

Electronic Nose: Current Status and Future Trends

2008

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“…The metabolites are detected with an array of conducting polymer gas sensors which undergo changes in resistance on exposure to specific gases (5,10,20,24,26). Work conducted by Chandiok et al (4) first demonstrated the possibility of using conducting polymer gas sensor arrays for the diagnosis of BV.…”

mentioning

confidence: 99%

Analysis of Vaginal Acetic Acid in Patients Undergoing Treatment for Bacterial Vaginosis

Chaudry¹,

Travers²,

Yuenger

et al. 2004

J Clin Microbiol

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A "gold standard" method for the diagnosis of bacterial vaginosis (BV) is lacking. The clinical criteria described by the Amsel technique are subjective and difficult to quantify. Alternatively, the reading of Gramstained vaginal smears by scoring techniques such as those that use the Nugent or Hay-Ison scoring systems is again subjective, requires expert personnel to perform the reading, and is infrequently used clinically. Recently, a new diagnostic device, the Osmetech Microbial Analyzer-Bacterial Vaginosis (OMA-BV), which determines a patient's BV status on the basis of measurement of the amount of acetic acid present in a vaginal swab specimen, was approved by the Food and Drug Administration. The present study uses the conducting polymer gas-sensing technology of OMA-BV to measure the concentration of acetic acid in the headspace above vaginal swab specimens from patients undergoing treatment for BV with metronidazole. In 97.8% of the cases the level of acetic acid detected fell sharply during the treatment period, crossing from above to below the diagnostic threshold of 900 ppm. The diagnosis obtained on the basis of the level of vaginal acetic acid was compared with the diagnoses obtained by use of the Amsel criteria and the Nugent scoring system both at the time of initial entry into the study and at the repeat samplings on days 7 and 14. The results obtained with OMA-BV showed overall agreements compared with the results of the Amsel and Nugent tests of 98 and 94%, respectively, for the 34 patients monitored through the treatment process. This provides further evidence that the measurement of vaginal acetic acid by headspace analysis with conducting polymer sensors is a valid alternative to present tests for the diagnosis of BV.Bacterial vaginosis (BV) is the most common cause of vaginitis symptoms among women of childbearing age. The condition affects 10 to 15% of women (13) in the general female population, but an incidence as high as 40% has been reported among women attending sexually transmitted clinics (16). BV is associated with an increased risk for a host of obstetric, gynecological, and neonatal complications, including postoperative infection following hysterectomy (22), miscarriage (15), preterm birth (17), postabortion pelvic inflammatory disease (8), plasma cell endometritis (21), and human immunodeficiency virus infection (28).BV is characterized by a change or an imbalance in the vaginal ecosystem, whereby the number of Lactobacillus species decreases and there is an overgrowth of organisms such as Gardnerella vaginalis and anaerobic organisms such as Mobiluncus spp. (31). The change in flora is accompanied by biochemical changes in the vaginal fluid, including increases in the concentrations of diamines (6) as well as those of polyamines and volatile organic acids (29,35). These biochemical markers were used for the detection of BV in this study. Treatment is directed at reducing the numbers of these bacteria by several of the different treatment regimens recommended, including treatme...

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“…Chandiok et al [10] first demonstrated the possibility of using conducting polymer gas sensor arrays for detecting BV. Subsequent work using headspace-gas chromatography-mass spectrometry (HS-GC-MS) demonstrated that acetic acid was the primary volatile compound produced from vaginal swabs and further studies using Osmetechs system have demonstrated its clinical utility [11].…”

Section: Bacterial Vaginosis (Bv)mentioning

confidence: 99%