Screening for hemostatic activities of popular Chinese medicinal herbs in vitro

Abstract

Aims:This study aimed to identify new hemostyptics by assessing the coagulation enhancing activity of 114 Chinese herbal extracts in vitro.Methods:Herbs were boiled in water for 30 min, filtered and then lyophilized filtrates (10 mg/mL) were dissolved in water. Coagulation was assayed as prothrombin time (PT). Plasma diluted in saline was incubated with each extract for 5 min and then PT reagent was added, followed by CaCl2 solution and the time taken to form clots was measured. Extracts that decreased coagulation time were regarded as containing active compounds. The abilities of extracts to activate Factor XII were assessed and the activated form of factor XII (XIIa) was resolved by SDS-PAGE and visualized by silver staining.Results:Coagulation time was obviously shortened by extracts of Alpinia Rhizome, Areca, Artemisia Leaf, Cassia Bark, Danshen Root, Ephedra Herb, Epimedium Herb, Forsythia Fruit, Great Burdock Achene, Moutan Bark, Perilla Herb, Red Paeony Root, Schizonepeta Spike, Senticosus Rhizome, Sweet Annie, Uncaria Thorn and Zanthoxylum Peel. Factor XII was obviously activated by extracts of Artemisia Leaf and Great Burdock Achene, and slightly by Perilla herb.Conclusion:Some popular Chinese medicinal herbs have potential as hemostatic agents and could thus be develope as new strategies for the treatment and prevention of bleeding.

Histomorphometric study of ethanolic extract of Graptophyllum pictum (L.) Griff. leaves on croton oil-induced hemorrhoid mice: A Javanese traditional anti-hemorrhoid herb

“…The results of the astringency assay clearly showed the positive effect of GPLE (Table 2). In addition, plasma recalcification time (PRT), which is an important parameter of blood coagulation (Abascal and Yarnell, 2005;Ohkura et al, 2015;Ream Nayal and M Yasser Abajy, 2015), has significantly reduced by the treatment with GPLE (0.46 ± 0.19 min) compared to that of control (2.12 ± 0.19 min) (Table 2). These results suggest that GPLE has beneficial features in stopping the bleeding of hemorrhoids by astringency and coagulation activities.…”

Potential applications of Chinese herbal medicines with hemostatic properties

“…Many plants and plant-derived agents have hemostatic activity and perhaps even more novel hemostatic substances in Chinese medical herbs await discovery. We screened extracts derived from 114 plant species in a library of common herbal crude extracts and identified potential hemostatic agents by measuring blood coagulation activities (61). Seventeen herbal extracts induced extrinsic blood coagulation.…”

Dragon’s Blood-Loaded Mesoporous Silica Nanoparticles for Rapid Hemostasis and Antibacterial Activity

“…Rapid and effective hemostatic agents are crucial in reducing blood loss and mortality [4][5][6]. Traditional Chinese medicine (TCM) is an important part of the prevention and treatment of hemorrhagic diseases [7][8][9]. The hemostatic mechanism of TCM is unique due to its multicomponent, multi-target and multi-pathway effects, as shown by modern research [10][11][12].…”