Screening for lactic acid bacteria capable of inhibiting Campylobacter jejuni in in vitro simulations of the broiler chicken caecal environment

Robyn, Joris; Rasschaert, Geertrui; Messens, Winy; Pasmans, Frank; Heyndrickx, Marc

doi:10.3920/bm2012.0021

Cited by 28 publications

(30 citation statements)

References 35 publications

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“…Whereas, in clinical settings with immune-compromised patients, E.faecalis can be considered an opportunistic pathogen [1], it has also been shown to impart beneficial effects to health. A recent in vitro study demonstrated that E. faecalis was inhibitory to C. jejuni MB 4185 infection under simulated broiler caecal condition [2]. An E.faecalis isolated from a healthy adult showed the highest probiotic activity when compared with over 70 other lactic acid bacteria (LAB) isolates, including lactobacilli and bifidobacteria [3].…”

Section: Introductionmentioning

confidence: 99%

Enterococcus faecalis from Healthy Infants Modulates Inflammation through MAPK Signaling Pathways

et al. 2014

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Colonizing commensal bacteria after birth are required for the proper development of the gastrointestinal tract. It is believed that bacterial colonization pattern in neonatal gut affects gut barrier function and immune system maturation. Studies on the development of faecal microbiota in infants showed that the neonatal gut was first colonized with enterococci followed by other microbiota such as Bifidobacterium. Other studies showed that babies who developed allergy were less often colonized with Enterococcus during the first month of life as compared to healthy infants. Many studies have been conducted to elucidate how bifidobacteria or lactobacilli, some of which are considered probiotic, regulate infant gut immunity. However, fewer studies have been focused on enterococi. In our study, we demonstrate that E. faecalis, isolated from healthy newborns, suppress inflammatory responses activated in vivo and in vitro. We found E. faecalis attenuates proinflammatory cytokine secretions, especially IL-8, through JNK and p38 signaling pathways. This finding shed light on how the first colonizer, E.faecalis, regulates inflammatory responses in the host.

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Section: Introductionmentioning

confidence: 99%

Enterococcus faecalis from Healthy Infants Modulates Inflammation through MAPK Signaling Pathways

et al. 2014

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“…For example, there is little available information regarding the relationship between chytrid growth inhibition in vitro and effective inhibition of fungal growth or the development of disease in vivo. Experimental efforts using probiotics to control Campylobacter in poultry show that the relationship will probably not be straightforward and that some bacteria that are inhibitory are ineffective against pre-existing infections [37,38]. Efficient and persistent host and environmental colonization needs to be established: amphibian skin microbiomes are dynamic and can be unstable and unpredictable, and bacterial community composition changes over the animal's lifetime [32].…”

Section: Trialled and Testedmentioning

confidence: 99%

Mitigating amphibian chytridiomycoses in nature

Garner

Schmidt²,

Martel

et al. 2016

Phil. Trans. R. Soc. B

135

156

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Amphibians across the planet face the threat of population decline and extirpation caused by the disease chytridiomycosis. Despite consensus that the fungal pathogens responsible for the disease are conservation issues, strategies to mitigate their impacts in the natural world are, at best, nascent. Reducing risk associated with the movement of amphibians, non-amphibian vectors and other sources of infection remains the first line of defence and a primary objective when mitigating the threat of disease in wildlife. Amphibian-associated chytridiomycete fungi and chytridiomycosis are already widespread, though, and we therefore focus on discussing options for mitigating the threats once disease emergence has occurred in wild amphibian populations. All strategies have shortcomings that need to be overcome before implementation, including stronger efforts towards understanding and addressing ethical and legal considerations. Even if these issues can be dealt with, all currently available approaches, or those under discussion, are unlikely to yield the desired conservation outcome of disease mitigation. The decision process for establishing mitigation strategies requires integrated thinking that assesses disease mitigation options critically and embeds them within more comprehensive strategies for the conservation of amphibian populations, communities and ecosystems. This article is part of the themed issue ‘Tackling emerging fungal threats to animal health, food security and ecosystem resilience’.

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“…72 However, the treatment of cell-free supernatants from these strains with proteinase K or chymotrypsin did not affect this activity, suggesting that something other than bacteriocins (e.g., organic acids) may be responsible. 72 Following protease and heat studies, the anti-Campylobacter activity of a Lactobacillus reuteri isolate was deemed unlikely to result from the production of bacteriocins, instead possibly resulting from the production of reuterin (3-hydroxypropionaldehyde), an antimicrobial metabolite. 73 Apart from lactobacilli, the other LAB most commonly associated with anti-Campylobacter activity are Enterococcus faecium and Enterococcus faecalis.…”

Section: Antimicrobial Peptides Active Against C Jejuni From Labmentioning

confidence: 98%

“…73 As with some Lactobacillus spp., there is evidence that some enterococci exert antagonism towards C. jejuni through means other than bacteriocins. 72 The anti-Campylobacter activity of certain strains of E. faecium and Lactobacillus pentosus was enhanced through the action of cellulase, although the source of this activity is not characterized. 77 Lactobacillus spp.…”

Section: Antimicrobial Peptides Active Against C Jejuni From Labmentioning

confidence: 99%

Characterization of bacterial antimicrobial peptides active against Campylobacter jejuni

Lohans

Belkum

et al. 2015

Can. J. Chem.

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Abstract:Campylobacter jejuni is one of the major causes of food poisoning, often resulting from the consumption of improperly cooked poultry products. The emergence of C. jejuni strains resistant to conventional antibiotics necessitates the evaluation of other possible treatments or preventative measures to minimize the impact and prevalence of infections. Antimicrobial peptides produced by bacteria have begun to emerge as a potential means of decreasing the levels of C. jejuni in poultry, thereby limiting Campylobacter contamination in associated food products. A number of bacteriocins produced by Gram-positive bacteria have unexpectedly been described as having antimicrobial activity against the Gram-negative C. jejuni. Additionally, some nonribosomal lipopeptides produced by Bacillus and Paenibacillus spp. show efficacy against this pathogen. This review will describe the bacterial antimicrobial peptides reported to be active against C. jejuni, with an emphasis on the characterization of their primary structures. However, for many of these peptides, little is known about their amino acid sequences and structures. Furthermore, there are unusual inconsistencies associated with the reported amino acid sequences for several of the more well-studied bacteriocins. Clarifying the chemical nature of these promising antimicrobial peptides is necessary before their potential utility for livestock protection from C. jejuni can be fully explored. Once these peptides are better characterized, they may prove to be strong candidates for minimizing the impact of Campylobacter on human health.Key words: Campylobacter, bacteriocin, antimicrobial, lipopeptide, tridecaptin. Résumé :Campylobacter jejuni est l'un des agents les plus importants à l'origine de l'empoisonnement alimentaire, souvent dû à la consommation de produits de volaille cuits de manière inadéquate. L'émergence de souches de C. jejuni résistantes aux antibiotiques habituels exige d'évaluer les autres possibilités de traitements ou mesures préventives afin de réduire la préva-lence et les conséquences des infections. La possibilité d'utiliser les peptides antimicrobiens d'origine bactérienne pour diminuer les taux de C. jejuni dans la volaille et limiter ainsi la contamination des produits alimentaires dérivés par le Campylobacter est apparue récemment. De façon inattendue, un certain nombre de bactériocines produites par des bactéries à Gram positif ont été décrites comme ayant une activité antimicrobienne contre la bactérie à Gram négatif C. jejuni. De plus, certains lipopeptides d'origine non ribosomique produits par des espèces de Bacillus et Paenibacillus montrent une efficacité contre cet agent pathogène. Cet exposé de synthèse décrira les peptides antimicrobiens d'origine bactérienne dont l'activité contre C. jejuni est connue, et mettra l'accent sur la caractérisation de leur structure primaire. Cependant, on sait peu de choses de la séquence d'acides aminés et de la structure de plusieurs de ces peptides. En outre, les séquences d'acides aminés de plusi...

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Screening for lactic acid bacteria capable of inhibiting Campylobacter jejuni in in vitro simulations of the broiler chicken caecal environment

Cited by 28 publications

References 35 publications

Enterococcus faecalis from Healthy Infants Modulates Inflammation through MAPK Signaling Pathways

Enterococcus faecalis from Healthy Infants Modulates Inflammation through MAPK Signaling Pathways

Mitigating amphibian chytridiomycoses in nature

Characterization of bacterial antimicrobial peptides active against Campylobacter jejuni

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