Screening for New Metabolites from Marine Microorganisms

Schweder, Thomas; Lindequist, Ulrike; Lalk, Michael

doi:10.1007/b135781

Cited by 16 publications

(18 citation statements)

References 115 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In metagenomic approaches functional gene clusters from uncultured organisms and communities of different organisms can be screened by isolating genetic material directly from original environmental samples, cloning and transformation of these sequences into a suitable host vector system and its expression in the host cells. Further omics technologies like proteome, transcriptome and metabolome analysis will help to understand biosynthesis and physiological importance of the interesting metabolites (Schweder et al ., 2005; Gross, 2009; Martins et al ., 2014). …”

Section: Discussionmentioning

confidence: 99%

“…Many biosynthetic genes remain silent and are not expressed in vitro . The exploitation of the whole biochemical potential of these organisms is an important challenge (Faulkner et al ., 2000; Schweder et al ., 2005; Gross, 2009; Marmann et al ., 2014; Martins et al ., 2014). In this context, the exploitation of the genomic information of whole communities, the so-called metagenome analysis, gets more and more importance.…”

Section: Marine Bioprospectingmentioning

confidence: 99%

“…The screening of microbial PUFA synthesis genes from metagenome libraries allowed the cloning of the responsible genes and the expression in suitable hosts like Escherichia coli and Synechococcus spp. (Takeyama et al, 1997; Schweder et al, 2005). …”

Section: Marine Compounds As Antihyperlipidemic Drugsmentioning

confidence: 99%

“…Most compounds have been isolated from marine invertebrates. Meanwhile, marine microorganisms attract more and more attention (Jensen and Fenical, 2000; Schweder et al ., 2005; Tarman et al ., 2013). …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Marine-Derived Pharmaceuticals - Challenges and Opportunities

Lindequist

2016

Biomolecules & Therapeutics

Self Cite

187

103

View full text Add to dashboard Cite

Marine biosphere is the largest one of the earth and harbors an enormous number of different organisms. Living conditions differ fundamentally from those in terrestrial environment. The production of specific secondary metabolites is an important adaption mechanism of marine organisms to survive in the sea. These metabolites possess biological activities which make them interesting as possible drugs for human. The review presents sources, chemistry, production and pharmacology of FDA approved marine derived pharmaceuticals arranged according to their therapeutic indication. Four of the presently seven approved drugs are used for the treatment of cancer. Each another one is applicated for treatment of viral diseases, chronic pain and to lower triglyceride level in blood. Some other products are of interest in diagnostic and as experimental tools. Besides, this article describes challenges in drug development from marine sources, especially the supply problem.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Marine Bioprospectingmentioning

confidence: 99%

Section: Marine Compounds As Antihyperlipidemic Drugsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Marine-Derived Pharmaceuticals - Challenges and Opportunities

Lindequist

2016

Biomolecules & Therapeutics

Self Cite

187

103

View full text Add to dashboard Cite

show abstract

“…In the marine environment, 2DE has been used to screen organisms for the presence of bioactive compounds and to detect and quantify changes in gene expression at the protein level during development, as well as in response to different physiological and environmental conditions (e.g. López et al 2001, 2002, 2005, Olsson et al 2004, Schweder et al 2005, Barneah et al 2006, McDonagh et al 2006. 2DE also has great potential for the study of genetic variability of populations, in that it allows a more representative sample of the genome to be analyzed.…”

Section: Proteomics Tools and Marine Ecologymentioning

confidence: 99%

Applications of proteomics in marine ecology

López¹

2007

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

Proteomics emerged in the beginning of the 1990s due to the need for new methods for protein analysis. Proteomics is a much newer discipline than genomics, and confronts similar challenges to those that genomics researchers faced in the implementation of large-scale sequencing programs. The definition of proteomics as the use of quantitative protein-level measurements of gene expression to characterize biological processes and decipher the mechanisms of gene expression control fits in with any biological approach. In the present study, proteomics is discussed and defined in parallel with genomics, given that many authors integrate proteomics in the context of functional genomics. In this Theme Section, several facets of proteomics in marine ecology were adressed: capacity, or what can be done, utility, the technology possibilities, and how to use the data obtained. As with any new and interesting technology, the expectations often exceed reality. The applications of proteomics, the advantages and disadvantages, as well as a few limitations are discussed.

show abstract

α-Glucosidase Inhibition and Antioxidant Properties of Streptomyces sp.: In Vitro

Kumar¹,

Raj²,

Christhudas³

et al. 2013

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Streptomyces strain isolated from the soil sediment was studied for its in vitro α-glucosidase and antioxidant properties. Morphological characterization and 16S rRNA partial gene sequencing were carried out to confirm that the strain Loyola AR1 belongs to genus Streptomyces sp. Modified nutrient glucose broth was used as the basal medium for growth and metabolites production. Ethyl acetate extract of Loyola AR1 (EA-Loyola AR1) showed 50% α-glucosidase inhibition at the concentration of 860.50 ± 2.68 μg/ml. Antioxidant properties such as total phenolic content of EA-Loyola AR1 was 176.83 ± 1.17 mg of catechol equivalents/g extracts. EA-Loyola AR1 showed significant scavenging activity on 2,2-diphenyl-picrylhydrazyl (50% inhibition (IC₅₀), 750.50 ± 1.61 μg/ml), hydroxyl (IC₅₀, 690.20 ± 2.38 μg/ml), nitric oxide (IC₅₀, 850.50 ± 1.77 μg/ml), and superoxide (IC₅₀, 880.08 ± 1.80 μg/ml) radicals, as well as reducing power. EA-Loyola AR1 showed strong suppressive effect on lipid peroxidation (IC₅₀, 670.50 ± 2.52 μg/ml). Antioxidants of β-carotene linoleate model system reveals significantly lower than butylated hydroxyanisole.

show abstract

Screening for New Metabolites from Marine Microorganisms

Cited by 16 publications

References 115 publications

Marine-Derived Pharmaceuticals - Challenges and Opportunities

Marine-Derived Pharmaceuticals - Challenges and Opportunities

Applications of proteomics in marine ecology

α-Glucosidase Inhibition and Antioxidant Properties of Streptomyces sp.: In Vitro

Contact Info

Product

Resources

About