Screening for Organic Phosphorus Compounds in Aquatic Sediments by Liquid Chromatography Coupled to ICP-AES and ESI-MS/MS

Brabandere, Heidi De; Forsgard, Niklas; Israelsson, Lena; Petterson, Jean; Rydin, Emil; Waldebäck, Monica; Sjöberg, Per J. R.

doi:10.1021/ac8006335

Cited by 22 publications

(12 citation statements)

References 39 publications

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“…The results show that Mt UPRT is specific for uracil, as no product formation could be detected for both cytosine and thymine bases (data not shown). This result was confirmed using liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) [38]. UPRT from several organisms were also shown to be specific for uracil and some uracil analogues [33], [36].…”

Section: Resultsmentioning

confidence: 70%

“…The injected sample volume was 20 µL, which was eluted isocratically with 10 mM ammonium acetate containing 40% acetonitrile at 0.8 ml min −1 flow rate. The LC detector was an ESI coupled to the 3200 Q-Trap (Applied Biosystems MDS SCIEX), employing the ESI-MS/MS parameters as described by others [38]. During the chromatography run, precursor ion scan (Prec) and enhance product ion scan (EPI) were monitored.…”

Section: Methodsmentioning

confidence: 99%

“…During the chromatography run, precursor ion scan (Prec) and enhance product ion scan (EPI) were monitored. Prec monitored precursors of mass over charge ratio ( m/z ) of compounds containing a phosphate group (H 2 PO 4 − , m/z 97) and EPI gave the fragmentation spectra of the nucleotides m/z [38].…”

Section: Methodsmentioning

confidence: 99%

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Biochemical Characterization of Uracil Phosphoribosyltransferase from Mycobacterium tuberculosis

et al. 2013

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Uracil phosphoribosyltransferase (UPRT) catalyzes the conversion of uracil and 5-phosphoribosyl-α-1-pyrophosphate (PRPP) to uridine 5′-monophosphate (UMP) and pyrophosphate (PPi). UPRT plays an important role in the pyrimidine salvage pathway since UMP is a common precursor of all pyrimidine nucleotides. Here we describe cloning, expression and purification to homogeneity of upp-encoded UPRT from Mycobacterium tuberculosis (MtUPRT). Mass spectrometry and N-terminal amino acid sequencing unambiguously identified the homogeneous protein as MtUPRT. Analytical ultracentrifugation showed that native MtUPRT follows a monomer-tetramer association model. MtUPRT is specific for uracil. GTP is not a modulator of MtUPRT ativity. MtUPRT was not significantly activated or inhibited by ATP, UTP, and CTP. Initial velocity and isothermal titration calorimetry studies suggest that catalysis follows a sequential ordered mechanism, in which PRPP binding is followed by uracil, and PPi product is released first followed by UMP. The pH-rate profiles indicated that groups with pK values of 5.7 and 8.1 are important for catalysis, and a group with a pK value of 9.5 is involved in PRPP binding. The results here described provide a solid foundation on which to base upp gene knockout aiming at the development of strategies to prevent tuberculosis.

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Section: Resultsmentioning

confidence: 70%

Section: Methodsmentioning

confidence: 99%

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Biochemical Characterization of Uracil Phosphoribosyltransferase from Mycobacterium tuberculosis

et al. 2013

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“…[58] Monophosphate nucleotides including 3 0 -AMP, 5 0 -AMP, cAMP, 5 0 -GTP, cytidine 5 0 -monophosphate and uridine 5 0 -monophosphate have been identified, but not quantified, in lake sediments from Sweden using liquid chromatography followed by electrospray ionisation tandem mass spectroscopy. [59] Quantification Where they have been measured, it would appear that simple nucleotides only represent a very small proportion of the phosphate pool present in the aquatic environment, with concentrations reported in the picograms to nanograms of phosphorous per litre range. For example, ATP and GTP represented only ,0.1 % of the organic P pool in the surface water in the North Pacific Ocean.…”

Section: Identificationmentioning

confidence: 99%

Organic phosphorus in the aquatic environment

Baldwin¹

2013

Environ. Chem.

104

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Environmental context. Organic phosphorus can be one of the major fractions of phosphorus in many aquatic ecosystems. This paper discusses the distribution, cycling and ecological significance of five major classes of organic P in the aquatic environment and discusses several principles to guide organic P research into the future.Abstract. Organic phosphorus can be one of the major fractions of phosphorus in many aquatic ecosystems. Unfortunately, in many studies the 'organic' P fraction is operationally defined. However, there are an increasing number of studies where the organic P species have been structurally characterised -in part because of the adoption of 31 P NMR spectroscopic techniques. There are five classes of organic P species that have been specifically identified in the aquatic environment -nucleic acids, other nucleotides, inositol phosphates, phospholipids and phosphonates. This paper explores the identification, quantification, biogeochemical cycling and ecological significance of these organic P compounds. Based on this analysis, the paper then identifies a number of principles which could guide the research of organic P into the future. There is an ongoing need to develop methods for quickly and accurately identifying and quantifying organic P species in the environment. The types of ecosystems in which organic P dynamics are studied needs to be expanded; flowing waters, floodplains and small wetlands are currently all under-represented in the literature. While enzymatic hydrolysis is an important transformation pathway for the breakdown of organic P, more effort needs to be directed towards studying other potential transformation pathways. Similarly effort should be directed to estimating the rates of transformations, not simply reporting on the concentrations. And finally, further work is needed in elucidating other roles of organic P in the environment other than simply a source of P to aquatic organisms.

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“…The precise characterization of various P species in the soil as a dynamic response to nonequilibrium conditions imposed by human activities, such as fertilization, can support a better understanding of reactivity, stability and particularly the plant accessibility of different P forms and provide a basis for best management practices. Several techniques, such as sequential fractionation (Dieter et al, 2010;Condron and Newman, 2011), nuclear magnetic resonance (NMR) spectroscopy (Liu et al, 2009;Vestergren et al, 2012;Ahlgren et al, 2013), Raman spectroscopy (Lanfranco, 2003;Vogel et al, 2013) and chromatography coupled to mass spectroscopy (De Brabandere et al, 2008;Paraskova et al, 2015), have been developed for P-speciation analysis in soil and sediments. Each one of these techniques can offer specific advantages and disadvantages depending on the phase and complexity of sample matrixes (Kruse et al, 2015).…”

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confidence: 99%

Bone char effects on soil: sequential fractionations and XANES spectroscopy

Morshedizad

Panten

Klysubun

et al. 2018

SOIL

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Abstract. The acceptability of novel bone char fertilizers depends on their P release, but reactions at bone char surfaces and impacts on soil P speciation are insufficiently known. By using sequential fractionation and synchrotron-based X-ray absorption near-edge structure (XANES) spectroscopy we investigated whether and how the chemical composition of bone char particles has been altered in soil and has consequently affected the P speciation of amended soils. Therefore, two different kinds of bone char particles (BC produced by the pyrolysis of degreased animal bone chips at 800 • C and BC plus , a BC enriched with reduced sulfur compounds) were manually separated from the soil at the end of two different experiments: incubation leaching and ryegrass cultivation. Sequential P fractionation of amended soils showed P enrichment in all fractions compared to the control. The most P increase between all treatments significantly occurred in the NaOH-P and resin-P fractions in response to BC plus application in both incubation-leaching and ryegrass cultivation experiments. This increase in the readily available P fraction in BC plus -treated soils was confirmed by linear combination fitting (LCF) analysis on P K-edge XANES spectra of BC particles and amended soils. The proportion of Ca hydroxyapatite decreased, whereas the proportion of CaHPO 4 increased in BC plus particles after amended soils had been incubated and leached and cropped by ryegrass. Based on P XANES speciation as determined by LCF analysis, the proportion of inorganic Ca(H 2 PO 4 ) 2 increased in amended soils after BC plus application. These results indicate that soil amendment with BC plus particles leads to elevated P concentration and maintains more soluble P species than BC particles even after 230 days of ryegrass cultivation.

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Screening for Organic Phosphorus Compounds in Aquatic Sediments by Liquid Chromatography Coupled to ICP-AES and ESI-MS/MS

Cited by 22 publications

References 39 publications

Biochemical Characterization of Uracil Phosphoribosyltransferase from Mycobacterium tuberculosis

Biochemical Characterization of Uracil Phosphoribosyltransferase from Mycobacterium tuberculosis

Organic phosphorus in the aquatic environment

Bone char effects on soil: sequential fractionations and XANES spectroscopy

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