Screening for Pseudomonas and Bacillus antagonistic rhizobacteria strains for the biocontrol of Fusarium wilt of chickpea

Abed, Hannane; Rouag, Noureddine; Mouatassem, Dahou; Rouabhi, Amar

doi:10.18393/ejss.2016.3.182-191

Cited by 21 publications

(15 citation statements)

References 35 publications

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“…Pseudomonadaceae) , and Clostridium (fam. Clostridiaceae) contain species with gelatinolytic capabilities (Whaley et al, 1982; Chakraborty, Mahapatra & Roy, 2011; Balan et al, 2012; Zhang et al, 2015; Abed et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Composition and dynamics of the bacterial communities present in the post-slaughter environment of farmed Atlantic salmon (Salmo salarL.) and correlations to gelatin degrading activity

Jacobsen

Mikalsen

Joensen

et al. 2019

PeerJ

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Background Microbial analyses performed in connection with the post-slaughter environment of farmed Atlantic salmon (Salmo salar L.) have mostly focused on specific bacteria that may have negative effects on the health of consumers. However, bacteria may also affect other quality variables. The objective of this study was to provide general knowledge about composition and dynamics of the bacterial communities present at slaughter and cold storage of farmed Atlantic salmon, as well as reveal any possible correlations to gelatinase activity, which may affect fillet quality. Thus, these data may provide a basis for optimization opportunities in the aquaculture industry. Methods Samples were taken from the digestive system harvested from 15 salmon immediately after slaughter. Another 17 salmon were taken from the processing line just before the final cleaning stage; of these eight were distributed in three iced storage boxes while the other nine were rinsed an extra time with industrial water before being distributed into another three storage boxes. In the following 6 days, samples were taken of skin mucus, liquids in the abdominal cavity and the storage ice. The compositions of the bacterial communities were analyzed by next-generation sequencing and gelatinase activity was measured in all samples except the storage ice. Results The bacterial communities in the digestive tract samples were dominated by the family Mycoplasmataceae. The genus Aliivibrio was also relatively abundant. Bacterial communities in the abdominal cavity were generally more diverse than the intestinal samples. However, all of the abdominal samples from storage box no. 3 had a high relative abundance of Mycoplasmataceae, and could not be distinguished from the intestinal samples (Q = 1.27, p = 0.633) while being significantly different from the other abdominal samples (Q = 9.02, p = 0.01). In addition, the abdominal samples from storage box no. 3 had a significantly higher gelatin degrading activity (Q = 9.43, p = 0.001) than those from the other storage boxes and similar to the high gelatinase activity in the intestinal samples. This indicated that in storage box no. 3 there was a transfer of intestinal fluids to the abdominal cavities, which was not removed by the cleaning procedure. There was a significant difference of the major phyla detected in the skin mucus of salmon rinsed an additional time, as these salmon had a higher relative amount of Firmicutes (F = 4.76, p = 0.04) and lower amount of Proteobacteria (F = 4.41, p = 0.047). Conclusions The study showed a correlation between intestinal fluids and bacteria left in the abdominal cavity and gelatinase activity. This suggested that intestinal fluids and/or bacteria could enhance the degradation of connective tissue in the abdominal cavity and hence negatively affect the fillet quality. In addition, the study provided general knowledge of the composition and dynamics of bacterial communities present.

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Section: Discussionmentioning

confidence: 99%

Composition and dynamics of the bacterial communities present in the post-slaughter environment of farmed Atlantic salmon (Salmo salarL.) and correlations to gelatin degrading activity

Jacobsen

Mikalsen

Joensen

et al. 2019

PeerJ

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“…The mechanism of action would be through direct antagonism such as production of bioactive metabolites, rapid exploitation of root exudates, colonization and multiplication in the environment and aggressive antagonism with other microbes (Thomashow and Weller, 1988). Abed et al (2016) tested Pseudomonas spp for their antagonism ability against Foc in vitro conditions. The results showed a great variability in inhibiting mycelial growth of Foc isolates.…”

Section: Discussionmentioning

confidence: 99%

Role of soil physicochemical and microbiological properties in the occurrence and severity of chickpea's Fusarium wilt disease

Moutassem

Belabid

Bellik

et al. 2019

Eurasian Journal of Soil Science (Ejss)

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The aim of the present study is to evaluate the relative disease severity of chickpea wilt in the most important chickpea growing areas in North Algeria and their relationship to soils properties. The physicochemical and biological parameters of 14 soils were analyzed and correlated to the disease index severity (Dis). Soil physicochemical factors were determined as a means of particle size distribution, pH, Electrical Conductivity (EC), CaCO3 content, total Nitrogen (Total-N), Olsen-P and biological factors including Foc inoculum density (ID-Foc), Trichoderma spp propagule number (TrPn), Pseudomonas spp and Bacillus spp. The results revealed that the spread of the disease was evident in all prospected areas and recorded as low to medium with values ranging from 2.05 to3 9.8. The disease severity was positively correlated with EC (r=0.62), Total-N (r= 0.79), and ID-Foc (r=0.72), whereas negatively correlated with Olsen-P (r=-0.67), TrPn (r=-0.70) and Pseudomonas spp (r=-0.89). There was no correlation between Dis and soil physical (clay, loam and sand), chemical (pH, CaCO3 content) and biological factors (Bacillus spp). As well, ID-Foc was positively correlated with Total-N and negatively correlated with Olsen-P. The results indicated that TrPn and Pseudomonas spp were positively correlated, whereas both were negatively associated with ID-Foc and Dis. Our finding pointed out the critical role of some physicochemical and biological soil characteristics in the epidemic development of chickpea wilt under field conditions.

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“…To elucida by this org directly in membrane hyphae of Abed et al (2016), in the search for rhizobacteria antagonistic against F. oxysporum f. sp. ciceris in chickpea, first made an in vitro selection from 140 bacterial strains to identify those with some biocontrol potential.…”

Section: Evaluation Of the Inhibitory Effect Of Non-volatile Metabolites Produced By Bacillus Sppmentioning

confidence: 99%

Biocontrol of Fusarium oxysporum f. sp. vasinfectum With Bacillus spp. Strains

Montalvão¹,

Castro²,

Blum³

et al. 2021

JAS

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The wilt caused by Fusarium oxysporum f.sp. vasinfectum in cotton is one of the main diseases of this crop. The pathogen is difficult to control because it produces chlamydospores, which allows it to survive in the soil for long periods without the presence of its main host. The control of this disease is based on varieties with some resistance, certified seeds, and fungicides used to treat seeds. Thus, the development of control alternatives is needed to increase the number of resources to manage this disease in the field. Bacillus are bioregulators and antagonists of several important phytopathogens. Therefore, the objective of this work was to select strains of Bacillus (Paenibacillus alvei, Bacillus thuringiensis, B. megaterium, and B. lentus), whose metabolites are toxic to F. oxysporum in laboratory and greenhouse conditions, in addition to adjusting in vitro methodologies to perform and evaluate selection tests. This work determined the best culture medium for the direct confrontation assays, proposed a grading scale to facilitate decisions about selection with the dual tests, identified among the strains selected in the confrontation assays those that produce volatile and non-volatile metabolites toxic to F. oxysporum, and tested these selected strains for disease control during seed germination in greenhouse trials. In last test, some strains of Bacillus had a potential protective effect during the germination of cotton seeds.

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Screening for Pseudomonas and Bacillus antagonistic rhizobacteria strains for the biocontrol of Fusarium wilt of chickpea

Cited by 21 publications

References 35 publications

Composition and dynamics of the bacterial communities present in the post-slaughter environment of farmed Atlantic salmon (Salmo salarL.) and correlations to gelatin degrading activity

Composition and dynamics of the bacterial communities present in the post-slaughter environment of farmed Atlantic salmon (Salmo salarL.) and correlations to gelatin degrading activity

Role of soil physicochemical and microbiological properties in the occurrence and severity of chickpea's Fusarium wilt disease

Biocontrol of Fusarium oxysporum f. sp. vasinfectum With Bacillus spp. Strains

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