Screening for Ptaquiloside in Ferns: Using Herbarium Specimens for Qualitative Mapping Purposes

Rasmussen, Lars Holm; Pedersen, Henrik Ærenlund

doi:10.1002/pca.2707

Cited by 17 publications

(12 citation statements)

References 21 publications

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“…The method for quanti cation of PTA and PTB was adapted from the method of Ayala-Luis et al and Rasmussen and Pedersen [31,42]. Quanti cation of PTA and PTB in bracken extracts took place on an Agilent 1200 series diode array detector (DAD) HPLC system equipped with a Phenomenex Hyperclone C8-DBS (150 mm x 4.6 mm, 3 µm) column thermostated at 35 o C; a Phenomenex Gemini C6-Phenyl guard column was used.…”

Section: Bracken Biomass: During the Entire Monitoring Period Brackementioning

confidence: 99%

“…PTA determination in soil: the extraction of PTA from soils was carried out following the method by Jensen et al [27]. After extraction, PTA was converted to PTB using the method by Rasmussen and Pedersen (2017) [42]. First, 6 mL of deionized water was added to each sample tube, followed by shaking on a atbed shaker for 10 minutes at 18 Hz.…”

Section: Bracken Biomass: During the Entire Monitoring Period Brackementioning

confidence: 99%

“…The cleaning up of impurities and PTB in the extract was done by passage through solid phase extraction columns dry packed with polyamide. The PTA in the ltered extract was then converted to PTB by stepwise addition of 75 µL of sodium hydroxide (1 M) and 75 µL of tri uoroacetic acid (2.5 M) [42].…”

Section: Bracken Biomass: During the Entire Monitoring Period Brackementioning

confidence: 99%

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Bracken Growth, Toxin Production and Transfer From Plant to Soil - A Two-year Monitoring Study

Garcia-Jorgensen

Diamantopoulos

Kisielius

et al. 2020

Preprint

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Background: Bracken fern (Pteridium aquilinum) is known to produce several toxic glycosides, of which ptaquiloside (PTA) is the most well documented. PTA may release from bracken to soil and leach to surface and groundwater. This study presents the first comprehensive long-term (2018-2019) monitoring study of bracken biomass, PTA content in the biomass, release by precipitation and soil solution concentrations at 50 cm depth. Moreover, lab experiments were carried to estimate the degradation of PTA, for different soil horizons and moisture contents. Results: The PTA content in bracken was highest at the beginning of the season, following a decreasing trend towards negligible values at the end of the season. The maximum seasonal PTA mass in the canopy was observed early in the summer, with values up to 1600 mg m-2. PTA is washed in high amounts by precipitation, with releases of up to 13.1 mg PTA m-2 during a single rain event. Concentrations of PTA in soil pore water were positively correlated with the mass of PTA in the canopy during the growing season. Peak concentrations of PTA in the soil solution were observed in July, with an average concentration of 1050 ng L-1.Conclusions: The production of PTA in bracken was found to be proportional to biomass production, while the mass of PTA being released is a function of volume and intensity of precipitation, as well as the bracken canopy development stage. Leaching of PTA takes place in the form of pulses linked to precipitation events, with soil pore water concentrations exceeding levels which are known to pose a risk to human health.

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Section: Bracken Biomass: During the Entire Monitoring Period Brackementioning

confidence: 99%

Section: Bracken Biomass: During the Entire Monitoring Period Brackementioning

confidence: 99%

Section: Bracken Biomass: During the Entire Monitoring Period Brackementioning

confidence: 99%

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Bracken Growth, Toxin Production and Transfer From Plant to Soil - A Two-year Monitoring Study

Garcia-Jorgensen

Diamantopoulos

Kisielius

et al. 2020

Preprint

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“…In general, the content of PTA is between 500 and 5,000 μg/g. The concentration is variable and generally highest among newly emerged crosiers [2,3,11–13].…”

Section: Introductionmentioning

confidence: 99%

“…PTA is converted to the aglycone ptaquilosin in aqueous solutions upon liberation of glucose and further on to PTB, which is relative stable under laboratory conditions, but rapidly degrades under influence of microorganisms [23]. The molar ratio between PTA and PTB is often smaller than 1 as by-products may form depending on the reaction conditions [3]. The conversion rate is depending on temperature and pH in dilute aqueous solutions.…”

Section: Introductionmentioning

confidence: 99%

Fate of ptaquiloside—A bracken fern toxin—In cattle

et al. 2019

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Ptaquiloside is a natural toxin present in bracken ferns ( Pteridium sp .). Cattle ingesting bracken may develop bladder tumours and excrete genotoxins in meat and milk. However, the fate of ptaquiloside in cattle and the link between ptaquiloside and cattle carcinogenesis is unresolved. Here, we present the toxicokinetic profile of ptaquiloside in plasma and urine after intravenous administration of ptaquiloside and after oral administration of bracken. Administered intravenously ptaquiloside, revealed a volume of distribution of 1.3 L kg -1 with a mean residence-time of 4 hours. A large fraction of ptaquiloside was converted to non-toxic pterosin B in the blood stream. Both ptaquiloside and pterosin B were excreted in urine (up to 41% of the dose). Oral administration of ptaquiloside via bracken extract or dried ferns did not result in observations of ptaquiloside in body fluids, indicating deglycosolidation in the rumen. Pterosin B was detected in both plasma and urine after oral administration. Hence, transport of carcinogenic ptaquiloside metabolites over the rumen membrane is indicated. Pterosin B recovered from urine counted for 7% of the dose given intravenously. Heifers exposed to bracken for 7 days (2 mg ptaquiloside kg -1 ) developed preneoplastic lesions in the urinary bladder most likely caused by genotoxic ptaquiloside metabolites.

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Does invasion by Pteridium aquilinum (Dennstaedtiaceae) affect the ecological succession in Atlantic Forest areas after a fire?

Carvalho

Zanúncio

et al. 2021

Environ Sci Pollut Res

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Screening for Ptaquiloside in Ferns: Using Herbarium Specimens for Qualitative Mapping Purposes

Abstract: Herbarium specimens up to 50 years old can be used for explorative risk assessment of ferns using HPLC-DAD for quantification and identification. Copyright © 2017 John Wiley & Sons, Ltd.

Cited by 17 publications

References 21 publications

Bracken Growth, Toxin Production and Transfer From Plant to Soil - A Two-year Monitoring Study

Bracken Growth, Toxin Production and Transfer From Plant to Soil - A Two-year Monitoring Study

Fate of ptaquiloside—A bracken fern toxin—In cattle

Does invasion by Pteridium aquilinum (Dennstaedtiaceae) affect the ecological succession in Atlantic Forest areas after a fire?

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