Screening fungi for synthesis of keratinolytic enzymes

Friedrich, Jožica; Gradišar, Helena; Mandin, D.; Chaumont, Jean-Pierre

doi:10.1046/j.1365-2672.1999.00485.x

Cited by 88 publications

(57 citation statements)

References 15 publications

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“…Keratinase assay. Keratinase activity was determined using method of Friedrich et al (1999) with some modifications. 0.25 mL of crude enzyme with 0.75 mL keratin powder (1% w/v) in 0.1 M glycine buffer pH 7.8 for half an hour at 40 o C in a shaking water bath.…”

Section: Methodsmentioning

confidence: 99%

Optimization of Extraction Parameters for Keratinase Recovery from Fermented Feather under Solid State Fermentation by Streptomyces sp. NRC 13S

Shata¹,

Farid²

2012

Journal of Applied Biological Chemistry

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The effects of solvent type and concentration, solid/ liquid ratio, extraction time and repeated extraction on recovery of keratinase from solid-state fermentation (SSF) of chicken feather by a local Streptomyces sp. NRC 13S were investigated in order to establish the experimental conditions for keratinase yield. Among solvents tested, 0.5% (v/v) glycerol was the best. BoxBehnken design was used to investigate the effect of relevant variables on keratinase recovery. The factors investigated were solid/liquid ratio (1:1.66-1:6.66 g/mL), glycerol concentration (0.5-5% v/v) and repeated extraction (1-5 cycle). The results showed that the maximum recovery of keratinase (6933.3 U/gfs) was obtained using 0.5 (v/v) glycerol as extracting solvent, in a solid /liquid ratio of 1:5 and three extraction cycles.

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Section: Methodsmentioning

confidence: 99%

Optimization of Extraction Parameters for Keratinase Recovery from Fermented Feather under Solid State Fermentation by Streptomyces sp. NRC 13S

Shata¹,

Farid²

2012

Journal of Applied Biological Chemistry

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“…-Feather preparation: The added feather was firstly defatted by soaking in chloroform-methanol (1:1 v/v) for 4 days with changing this solvent once a day then washed by water which subsequently air drying until getting a constant weight (Friedrich et al, 1999). …”

Section: Screening For Feather Degradationmentioning

confidence: 99%

“…Keratin is a structural protein forming the mammalian outer most tissues like wool, nails and horns, and it could be considered as the main component of birds feather (Friedrich, Gradišar, Mandin, & Chaumont, 1999). Keratins are two types, α and β types, whereas, α keratins are two types; the soft α keratin found in epidermis and other epithelial tissues and the hard α keratins found in nail, hair, wool , claw, hoof and the horns of cattle, goats and sheep (Marshall, Orwin, & Gillespie, 1991).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Keratin Degrading Alkaliphiles

Elsayed

Mowafy

Elrazak

et al. 2016

JBLS

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Feather which mainly contains Keratin is one of the main poultry industry wastes and at the same time with high nutritional value if converted to soluble form. In this study, we isolated and characterized four alkaliphilic bacteria for their ability to degrade feather under alkaline conditions. Two of the isolates have been identified as Lysinibacillus spp. and both of them are growing optimally at 30 °C and pH 9. The others were identified as Micrococcus sp. and Nocardiopsis sp. and both of them showed optimum growth at 37 °C and pH 10.5. All the isolates are promising and potential tools that could be used for keratin degradation.

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“…and Penicillium spp. (Friedrich et al, 1999;Soomro et al, 2012;Ramakrishnaiah et al, 2013;Singh, 2014;Singh et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Mycoflora associated with the goats hair and sheep wool in Taif, Saudi Arabia

Awad¹

2017

Afr. J. Microbiol. Res.

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The objective of this study was to evaluate the occurrence of mycoflora in 30 samples of healthy goat's hair and sheep wool collected from different localities in Taif, Saudi Arabia, and the ability of some fungal isolates for keratinase activities. Sixty four species belonging to 28 genera were collected from the two substrates. The wool of sheep was polluted with fungi than goat hairs, and contained high total counts and number of fungal genera and species. Nine species of true dermatophytes isolated belonged to Microsporum (3 species) and Trichophyton (6 species). Several keratinophilic species were isolated of which, Chrysosporium indicum, Chrysosporium keratinophilum and Chrysosporium tropicum were the most prevalent. The commonest saprophytes in order of frequency were members of the genera, Aspergillus, Penicillium, Alternaria and Cochliobolus. In addition, the other genera found included Acremonium, Chaetomum, Cladosporium, Cochliobolus, Fusarium, Mucor, Paecilomyces, Phoma, Rhizopus, Scopulariopsis, Stachybotrys, Trichoderma and others. Five species from 20 tested isolates (Aspergillus niger, C. keratinophilum, C. tropicum, Microsporum gypseum and Trichoderma viride) had high keratinase activity. The results of this study indicate that both goat hair and sheep wool provide a suitable habitat for dermatophytes and other keratinophilic fungi. Most of these fungi play an important role in the degradation of keratin substrates, so that they can help preserve the environment and reduce pollution.

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Screening fungi for synthesis of keratinolytic enzymes

Cited by 88 publications

References 15 publications

Optimization of Extraction Parameters for Keratinase Recovery from Fermented Feather under Solid State Fermentation by Streptomyces sp. NRC 13S

Optimization of Extraction Parameters for Keratinase Recovery from Fermented Feather under Solid State Fermentation by Streptomyces sp. NRC 13S

Characterization of Keratin Degrading Alkaliphiles

Mycoflora associated with the goats hair and sheep wool in Taif, Saudi Arabia

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