Screening heterotrophic microalgal strains by using the Biolog method for biofuel production from organic wastewater

Zhang, Tianyuan; Wu, Yin-Hu; Zhuang, Lin-Lan; Wang, Xiaoxiong; Hu, Hong-Ying

doi:10.1016/j.algal.2014.10.003

Cited by 50 publications

(15 citation statements)

References 19 publications

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“…In general, carbohydrates and carboxylic acids represented the two highest amounts of carbon sources utilized across all biofilm samples. The high consumption of carbohydrates has also been previously reported in the literature [39,40]. Sterols and amines were consumed the least of the six categories after NP exposure.…”

Section: Resultssupporting

confidence: 76%

“…All 31 carbon sources can be divided into six categories, including carbohydrates, amino acids, esters, sterols, amines, and carboxylic acids, based on biochemical properties and molecular composition, which paves the way to estimate metabolic functions in a physiologically relevant approach [39]. The extent of the utilization of specific carbon source categories by biofilms treated by NPs was analyzed and is displayed in Figure 2.…”

Section: Resultsmentioning

confidence: 99%

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Effects of Nanoplastics on Freshwater Biofilm Microbial Metabolic Functions as Determined by BIOLOG ECO Microplates

Miao

Guo

Liu

et al. 2019

IJERPH

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Nanoplastic (NP) contamination is becoming a pervasive issue as NPs, originating from microplastic particles, pose potentially harmful environmental impacts on aquatic ecosystems. The environmental hazards of NPs on microorganisms have been well documented in recent studies; however, little is known about their ecotoxicity effects on freshwater biofilms, which serve as important primary producers and decomposers and are highly connected with other ecosystem components. We investigated the effects of NPs on the microbial metabolic functions of freshwater biofilms in terms of carbon source utilization ability. Biofilm samples were collected, cultivated in a hydrodynamic flume for six weeks, and then exposed in polystyrene (PS) beads (100 nm in size) with different NP concentrations (1, 5, and 10 mg/L). BIOLOG ECO microplates were used to quantify carbon source utilization characteristics. The data were analyzed using average well-color development (AWCD), functional diversity indices, and principle component analysis (PCA). Results showed that the total carbon metabolic functions (represented by AWCD) remained constant (p > 0.05) with elevated NP concentrations, but some specific carbon sources (e.g., esters) changed in their utilization ability (p < 0.05). The microbial functional diversity (Shannon–Wiener diversity index, Simpson diversity index, and Shannon evenness index) was significantly reduced under 10 mg/L NPs (p < 0.05), indicating an inhibiting effect of NPs on biofilm metabolic diversity. This study examined NP ecotoxicity effects on microbial metabolic activities at the community level, but further studies are required to fully understand the mechanisms driving this change.

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Section: Resultssupporting

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Effects of Nanoplastics on Freshwater Biofilm Microbial Metabolic Functions as Determined by BIOLOG ECO Microplates

Miao

Guo

Liu

et al. 2019

IJERPH

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“…The carbon metabolism capacity of the sediment microbial community reached the maximum (K) in 168 h (7 d). Generally, it took an average of seven days, and at least five days, for the metabolic capacity of microorganisms to reach its maximum value, which is consistent with previous studies [32,64]. The average absorbance in the BIOLOG ECO microplate increased significantly, which indicated that the microbial communities in the four experimental conditions could use the carbon source in the BIOLOG ECO microplate during the stable period.…”

Section: The Awcd Of All Carbon Sources In Sediment Microbial Communisupporting

confidence: 90%

Microbial Carbon Metabolic Functions in Sediments Influenced by Resuspension Event

Zhang

Ding

et al. 2020

Water

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Microorganisms in sediments are an important part of the aquatic ecosystem, and their functional activities are sensitive to external environmental pressure. Shallow lakes are characterized by frequent sediment resuspension events, leading to large amounts of nutrients being released. However, information about the potential impacts of sediment resuspension events on the functional activities of microbial communities is limited. In this study, the responses of microbial carbon metabolism in sediments under different wind–wave disturbance were analyzed by BIOLOG ECO microplates. The results showed that under four disturbance conditions (wind speeds of 0, 1.60, 3.62, and 14.10 m/s), the total carbon metabolism function of the sediment microbial community (represented as average well-color development, AWCD) remained unchanged (p > 0.05), and the final total AWCD value stabilized at about 1.70. However, compared with the control group, some specific carbon sources (e.g., amines and carboxylic acids) showed significant changes (p < 0.05). We found that short-term (8 h) resuspension events did not affect the total carbon metabolism of sediment microbial communities, while it affected the microbial utilization ability of some specific types of carbon sources. For example, we found that the microbial utilization capacity of polymers in the 14.10 m/s group was the best. This study provides a new insight into the carbon cycle process of shallow lake sediments that resuspension events will affect the carbon cycle process of sediments.

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“…in a pilot-scale 16 m 2 open pond could remove 90% of the COD of an urban wastewater (from 180 to less than 20 mgO 2 /L, [21]). More recently, microalgae have been shown to grow on different carbon substrates in wastewater open ponds, from simple molecules (glucose, lactose) up to quite complex ones (α-cyclodextrin, Tween 40 and 80) [22]. This study showed that out of the five strains of green algae (3 Scenedesmus sp.…”

Section: Urban Wastewater Treatmentmentioning

confidence: 89%

The Environmental Biorefinery: Using Microalgae to Remediate Wastewater, a Win-Win Paradigm

et al. 2016

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Abstract:Microalgae have been shown to be a source of multiple bio-based products ranging from high value molecules to commodities. Along with their potential to produce a large variety of products, microalgae can also be used for the depollution of wastewaters of different origins (urban, industrial, and agricultural). This paper is focused on the importance of harnessing the bioremediation capacity of microalgae to treat wastewaters in order to develop the microalgae industry (especially the microalgae biofuel industry) and to find other alternatives to the classic wastewater treatment processes. The current research on the potential of microalgae to treat a specific wastewater or a targeted pollutant is reviewed and discussed. Then, both strategies of selecting the best microalgae strain to treat a specific wastewater or pollutant and using a natural or an artificial consortium to perform the treatment will be detailed. The process options for treating wastewaters using microalgae will be discussed up to the final valorization of the biomass. The last part is dedicated to the challenges which research need to address in order to develop the potential of microalgae to treat wastewaters.

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Screening heterotrophic microalgal strains by using the Biolog method for biofuel production from organic wastewater

Cited by 50 publications

References 19 publications

Effects of Nanoplastics on Freshwater Biofilm Microbial Metabolic Functions as Determined by BIOLOG ECO Microplates

Effects of Nanoplastics on Freshwater Biofilm Microbial Metabolic Functions as Determined by BIOLOG ECO Microplates

Microbial Carbon Metabolic Functions in Sediments Influenced by Resuspension Event

The Environmental Biorefinery: Using Microalgae to Remediate Wastewater, a Win-Win Paradigm

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