Screening, identification and characterisation of Polyhydroxybutyrate producing bacteria from garden soil

Abstract

Plastic pollution need to be resolved as it affects air, water, land. The favourable alternative for plastics would be Polyhydroxybutyrate (PHB) from bacterial origin, which are biodegradable and biocompatible biopolymers. Focus on the PHB producing bacteria is done by collecting garden soil sample. Five colonies of Sudan black blue positive isolates were chosen, extracted, and produced. One of the strains (SM1) - a potent producer as confirmed by crotonic acid assay, was further subjected to large scale production. The PHB thus produced was analysed using Fourier Transform Infra-Red spectroscopy (FTIR) and Gas Chromatography Mass Spectrometry (GCMS) to confirm the presence of functional groups. X-Ray crystallography revealed that it is of crystalline nature and are pictured by Scanning electron microscopy photography. DNA was isolated from the strain SM1, and the gene for 16S rRNA has been sequenced and submitted in GENBANK, (Accession No: MZ363886). The organism was found to be Bacillus cereus as predicted by 16S rRNA and NCBI BLAST. A phylogenetic tree was constructed using MEGA software. Bioplastic preparation was done under laboratory scale and the produced bioplastic was successfully degraded using Pseudomonas species. The prepared bioplastic from bacteria was biodegradable and eco-friendly.

Effect of Vermicompost Production on some Soil Properties and Nutrients in Plants

“…These are appropriate values for the availability of nutrients in the soil for plant growth. On the other hand, the results of the vermicompost analysis also showed a decrease in the electrical conductivity values, as it reached 0.50 dSm -1 and 2.99 dSm -1 in vermicompost produced from imported and local earthworms, respectively, and this is due to The genetic characteristics of the imported worms differ from the local ones, as well as the imported worms adapting to the production of vermicompost because they have already been used in the production of vermicompost before being used in this experiment, and this is close to what was stated in studies of [16,17]. the addition of vermicompost had a clear significant effect on the decrease in pH after planting, as the vermicompost treatment produced from imported earthworms reached 7.05, a decrease of 3.55% compared to the comparison treatment.…”

Unveiling the Power Microbial Degradation by Plastic-Degrading Microorganisms

“…Plastics are cheap petrochemical material, which are used in daily household ubiquitously (1,6). Plastic is more affordable, thermal, flexible, robust and electromagnetic insulator material (2). Plastics are widely used in construction material, food packaging packets, disposable cutleries, cassette disc because of its good properties and cheap cost (3).…”

Phenotypic and Genetic Characterization of Polyhydroxyalkanoate Producing Bacterial Isolates from Hypersaline Water Body, Atlantic Ocean, Nigeria

“…The preference for bioplastic over synthetic plastics is hinged on the fact that, the latter is non-biodegradable with disposal methods of burning which results in air pollution through the emission of toxic fumes that are inimical to human and animal health. Consequently, this action results in the disruption of the ecosystems and eventual global warming [10]. However, biopolymers (PHA) will not contribute to environmental pollution at the end of their use when recycled due to their elastomeric property [11,12 and 13].…”