Screening, identification and production of polyhydroxyalkanoates (PHAs)by sucrose utilizing microbes isolated from soil environments

Tanamool, Varavut; Imai, Tsuyoshi; Danvirutai, Paiboon; Kaewkannetra, Pakawadee

doi:10.1016/j.jbiotec.2010.09.419

Cited by 9 publications

(10 citation statements)

References 2 publications

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“…Ralstonia eutropha , which has been extensively used for PHA production, cannot use sucrose directly [ 21 ]. However, the following wild-type bacterial strains have been identified to produce PHAs from sucrose: Azotobacter vinelandii [ 22 ], Alcaligenes latus [ 23 ] and Hydrogenophaga pseudoflava [ 24 ]. These microorganisms can hydrolyse sucrose extracellularly in the early stage of cultivation into glucose and fructose, and both are subsequently used for cell growth [ 25 ].…”

Section: Carbohydratesmentioning

confidence: 99%

Carbon Sources for Polyhydroxyalkanoates and an Integrated Biorefinery

Jiang

Hill

Kowalczuk

et al. 2016

IJMS

190

109

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Polyhydroxyalkanoates (PHAs) are a group of bioplastics that have a wide range of applications. Extensive progress has been made in our understanding of PHAs’ biosynthesis, and currently, it is possible to engineer bacterial strains to produce PHAs with desired properties. The substrates for the fermentative production of PHAs are primarily derived from food-based carbon sources, raising concerns over the sustainability of their production in terms of their impact on food prices. This paper gives an overview of the current carbon sources used for PHA production and the methods used to transform these sources into fermentable forms. This allows us to identify the opportunities and restraints linked to future sustainable PHA production. Hemicellulose hydrolysates and crude glycerol are identified as two promising carbon sources for a sustainable production of PHAs. Hemicellulose hydrolysates and crude glycerol can be produced on a large scale during various second generation biofuels’ production. An integration of PHA production within a modern biorefinery is therefore proposed to produce biofuels and bioplastics simultaneously. This will create the potential to offset the production cost of biofuels and reduce the overall production cost of PHAs.

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Section: Carbohydratesmentioning

confidence: 99%

Carbon Sources for Polyhydroxyalkanoates and an Integrated Biorefinery

Jiang

Hill

Kowalczuk

et al. 2016

IJMS

190

109

View full text Add to dashboard Cite

show abstract

“…Some bacterial spp. such as Azotobbacter vinelandii, Alcaligens latus, and Hydrogenophage pseudoflawa have been identified as PHA-producing bacterial species [56,57]. PHA-producing isolate has shown higher PHA producers.…”

Section: Discussionmentioning

confidence: 99%

Polyhydroxyalkanoate recovery from newly screened Bacillus sp. LPPI-18 using various methods of extraction from Loktak Lake sediment sample

Mohammed

Ray

2022

Journal of Genetic Engineering and Biotechnology

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Background Nowadays, the conventional plastic wastes are very challenging to environments and its production cost also creates an economic crisis due to petrochemical-based plastic. In order to solve this problem, the current studies were aimed at screening and characterizing these polyhydroxyalkanoate (PHA)-producing isolates and evaluating the suitability of some carbon source for newly screened PHA-producing isolates. Material and methods Some carbon sources such as D-fructose, glucose, molasses, D-ribose and sucrose were evaluated for PHA production. Data were analyzed using SPSS version 20. The 16SrRNA gene sequence of these isolates was performed. These newly isolated taxa were related to Bacillus species. It was designated as Bacillus sp. LPPI-18 and affiliated Bacillus cereus ATCC 14577T (AE01687) (99.10%). Paenibacillus sp. 172 (AF273740.1) was used as an outgroup. Results Bacillus sp. LPPI-18 is a gram-positive, rod-shaped, endospore former, and citrate test positive. This isolate showed positive for amylase, catalase, pectinase, and protease test. They produced intracellular PHA granules when this isolate was stained with Sudan Black B (SBB) and Nile blue A (NBA) preliminary and specific staining dyes, respectively. Both temperature and pH used to affect polyhydroxyalkanoates (PHA) productivity. Bacteria are able to reserve PHA in the form of granules during stress conditions. This isolate produces only when supplied with carbon sources. More PHA contents (PCs) were obtained from glucose, molasses, and D-fructose. In this regard, the maximum mean value of PC was obtained from glucose (40.55±0.7%) and the minimum was obtained from D-ribose (12.4±1.4%). Great variations (P≤0.05) of PCs were observed among glucose and sucrose, molasses and sucrose, and D-fructose and sucrose carbon sources for PHA productivity (PP) of cell dry weight (CDW) g/L. After extraction, PHA film was produced for this typical isolate using glucose as a sole carbon source. Fourier transform infrared spectrum was performed for this isolate and showed the feature of polyester at 1719.64 to 1721.16 wavelengths for these extracted samples. The peak of fingerprinting (band of carboxylic acid group) at this wavelength is a characteristic feature of polyhydroxybutyrate (PHB) and corresponds to the ester functional group (C=O). Conclusion In this study, newly identified Bacillus sp. LPPI-18 is found to be producing biodegradable polymers that are used to replace highly pollutant conventional plastic polymers. This isolate is also used to employ certain cost-effective carbon sources for the production of PHA polymers. Graphical Abstract

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“…Some bacterial sp. such as Azotobbacter vinelandii, Alcaligens latus, and Hydrogenophage pseudo awa have been identi ed as PHA producing bacterial species (Grothe et al 1999;Park et al 2004b;Tanamool et al 2010). When Azotobacter vinelandii strain UWD supplied with 1 % fructose, glucose, maltose, and sucrose, > 2 mg/ml poly-β-hydroxybutyrate (PHB) was produced during exponential growth which is more yield than the present our results (Page 1989).…”

Section: Pha Productivity Against Molasses (1%) As a Carbon Sourcementioning

confidence: 53%

Polyhydroxyalkanoate Recovery From Newly Screened Bacillus Sp. LPPI-81 Using Various Methods of Extraction From Loktak Lake Sediment Sample

Ebu

Ray

2021

Preprint

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Nowadays the conventional plastic wastes are very challenging to environments and its production cost also creates an economic crisis due to petrochemical-based plastic. In order to solve this problem, the current studies were aimed at screening and characterizing these PHA producing isolates and evaluating the suitability of some carbon source for newly screened PHA producing isolates. Some carbon sources such as D-fructose, glucose, molasses, D-ribose and sucrose were evaluated for PHA production. Data were analyzed using SPSS version 20. The 16SrRNA gene sequence of these isolates was performed. This newly isolated taxa was related to Bacillus species. It was designed as Bacillus sp. LPPI-18 and affiliated Bacillus cereus ATCC 14577T (AE01687) (99.10%). Paenibacillus sp. 172 (AF273740.1) was used as an out-group. Bacillus sp. LPPI-18 is a gram-positive, rod-shaped, endospore former, and citrate test positive. This isolate showed positive for amylase, catalase, pectinase, and protease test. They produced intracellular PHA granules when this isolate was stained with Sudan Black B (SBB) and Nile Blue A (NBA) preliminary and specific staining dyes, respectively. Both Temperature and pH used to affect PHA productivity. Bacteria are able to reserve PHA in the form of granules during stress conditions. This isolate produces only when supplied with carbon sources. More PHA contents (PCs) were obtained from glucose, molasses, and D-fructose. In this regard, the maximum mean value of PC was obtained from glucose (40.55±0.7%) and the minimum was obtained from D-Ribose (12.4±1.4%). Great variations (p≤0.05) of PCs were observed among glucose & sucrose, molasses & sucrose and D-fructose & sucrose carbon sources for PHA productivity (PP) of Cell Dry Weight (CDW) g/L. After extraction, PHA film was produced for this typical isolate using glucose as a sole carbon source. Fourier transform infrared spectrum was performed for this isolate and showed the feature of polyester at 1719.64 to 1721.16 wavelength for these extracted samples. The peak of fingerprinting (band of carboxylic acid group) at this wave-length is a characteristic feature of PHB and corresponds to the ester functional group (C=O).

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Screening, identification and production of polyhydroxyalkanoates (PHAs)by sucrose utilizing microbes isolated from soil environments

Cited by 9 publications

References 2 publications

Carbon Sources for Polyhydroxyalkanoates and an Integrated Biorefinery

Carbon Sources for Polyhydroxyalkanoates and an Integrated Biorefinery

Polyhydroxyalkanoate recovery from newly screened Bacillus sp. LPPI-18 using various methods of extraction from Loktak Lake sediment sample

Polyhydroxyalkanoate Recovery From Newly Screened Bacillus Sp. LPPI-81 Using Various Methods of Extraction From Loktak Lake Sediment Sample

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